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Science 10 December 1999:
Vol. 286. no. 5447, pp. 2179 - 2184
DOI: 10.1126/science.286.5447.2179
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Reports

Microarray Analysis of Drosophila Development During Metamorphosis

Kevin P. White, * Scott A. Rifkin, dagger Patrick Hurban, ddagger David S. Hogness

Metamorphosis is an integrated set of developmental processes controlled by a transcriptional hierarchy that coordinates the action of hundreds of genes. In order to identify and analyze the expression of these genes, high-density DNA microarrays containing several thousand Drosophila melanogaster gene sequences were constructed. Many differentially expressed genes can be assigned to developmental pathways known to be active during metamorphosis, whereas others can be assigned to pathways not previously associated with metamorphosis. Additionally, many genes of unknown function were identified that may be involved in the control and execution of metamorphosis. The utility of this genome-based approach is demonstrated for studying a set of complex biological processes in a multicellular organism.

Department of Developmental Biology, Beckman Center B300, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
*   To whom correspondence should be addressed at the Department of Biochemistry, Beckman Center B400, Stanford University Medical Center, Stanford, CA 94305-5427, USA.

dagger    Present address: Department of Ecology and Evolutionary Biology, Yale University, Post Office Box 208106, New Haven, CT 06520, USA.

ddagger    Present address: Paradigm Genetics, 104 Alexander Drive, Building 2, Post Office Box 14528, Research Triangle Park, NC 27709-5428, USA.

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