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Science 26 November 1999:
Vol. 286. no. 5445, pp. 1729 - 1731
DOI: 10.1126/science.286.5445.1729
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Reports

A Breakdown of Brassica Self-Incompatibility in ARC1 Antisense Transgenic Plants

Sophia L. Stone, 1 MaryAnne Arnoldo, 2 Daphne R. Goring 1*

Self-incompatibility, the rejection of self pollen, is the most widespread mechanism by which flowering plants prevent inbreeding. In Brassica, the S receptor kinase (SRK) has been implicated in the self-incompatibility response, but the molecular mechanisms involving SRK are unknown. One putative downstream effector for SRK is ARC1, a protein that binds to the SRK kinase domain. Here it is shown that suppression of ARC1 messenger RNA levels in the self-incompatible Brassica napus W1 line is correlated with a partial breakdown of self-incompatibility, resulting in seed production. This provides strong evidence that ARC1 is a positive effector of the Brassica self-incompatibility response.

1 Department of Biology, York University, Toronto, Ontario, Canada M3J 1P3.
2 Pioneer Hi-Bred Production, Canola Research Station, Georgetown, Ontario, Canada L7G 4S7.
*   To whom correspondence should be addressed. E-mail: dgoring{at}yorku.ca

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