Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 19 November 1999:
Vol. 286. no. 5444, pp. 1583 - 1587
DOI: 10.1126/science.286.5444.1583
Prev | Table of Contents

Reports

Regulation of Myosin Phosphatase by a Specific Interaction with cGMP- Dependent Protein Kinase Ialpha

Howard K. Surks, 1 Naoki Mochizuki, 12 Yasuyo Kasai, 1 Serban P. Georgescu, 1 K. Mary Tang, 1 Masaaki Ito, 3 Thomas M. Lincoln, 4 Michael E. Mendelsohn 1*

Contraction and relaxation of smooth muscle are regulated by myosin light-chain kinase and myosin phosphatase through phosphorylation and dephosphorylation of myosin light chains. Cyclic guanosine monophosphate (cGMP)-dependent protein kinase Ialpha (cGKIalpha ) mediates physiologic relaxation of vascular smooth muscle in response to nitric oxide and cGMP. It is shown here that cGKIalpha is targeted to the smooth muscle cell contractile apparatus by a leucine zipper interaction with the myosin-binding subunit (MBS) of myosin phosphatase. Uncoupling of the cGKIalpha -MBS interaction prevents cGMP-dependent dephosphorylation of myosin light chain, demonstrating that this interaction is essential to the regulation of vascular smooth muscle cell tone.

1 Molecular Cardiology Research Institute and Cardiology Division, Department of Medicine, Tufts University School of Medicine and New England Medical Center, Boston, MA 02111, USA.
2 Department of Pathology, Research Institute, International Medical Center of Japan, Tokyo 162-8655, Japan.
3 First Department of Internal Medicine, Mie University School of Medicine, Tsu 514, Japan.
4 Department of Pathology, Division of Molecular and Cellular Pathology, University of Alabama, Birmingham, AL 35294-0019, USA.
*   To whom correspondence should be addressed. E-mail: mmendelsohn{at}lifespan.org

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Cell Signaling in Space and Time: Where Proteins Come Together and When They're Apart.
J. D. Scott and T. Pawson (2009)
Science 326, 1220-1224
   Abstract »    Full Text »    PDF »
Calcium-dependent and calcium-independent inhibition of contraction by cGMP/cGKI in intestinal smooth muscle.
E. Frei, M. Huster, P. Smital, J. Schlossmann, F. Hofmann, and J. W. Wegener (2009)
Am J Physiol Gastrointest Liver Physiol 297, G834-G839
   Abstract »    Full Text »    PDF »
Phosphorylation-dependent Autoinhibition of Myosin Light Chain Phosphatase Accounts for Ca2+ Sensitization Force of Smooth Muscle Contraction.
A. Khromov, N. Choudhury, A. S. Stevenson, A. V. Somlyo, and M. Eto (2009)
J. Biol. Chem. 284, 21569-21579
   Abstract »    Full Text »    PDF »
Nitric oxide-induced biphasic mechanism of vascular relaxation via dephosphorylation of CPI-17 and MYPT1.
T. Kitazawa, S. Semba, Y. H. Huh, K. Kitazawa, and M. Eto (2009)
J. Physiol. 587, 3587-3603
   Abstract »    Full Text »    PDF »
Cyclic Guanosine Monophosphate Signaling Cascade Mediates Pigment Aggregation in Freshwater Shrimp Chromatophores.
M. R. Ribeiro and J. C. McNamara (2009)
Biol. Bull. 216, 138-148
   Abstract »    Full Text »    PDF »
Thromboxane A2-induced Bi-directional Regulation of Cerebral Arterial Tone.
R. L. Neppl, L. T. Lubomirov, K. Momotani, G. Pfitzer, M. Eto, and A. V. Somlyo (2009)
J. Biol. Chem. 284, 6348-6360
   Abstract »    Full Text »    PDF »
ROCK Isoform Regulation of Myosin Phosphatase and Contractility in Vascular Smooth Muscle Cells.
Y. Wang, X. R. Zheng, N. Riddick, M. Bryden, W. Baur, X. Zhang, and H. K. Surks (2009)
Circ. Res. 104, 531-540
   Abstract »    Full Text »    PDF »
Prolonged treatment of porcine pulmonary artery with nitric oxide decreases cGMP sensitivity and cGMP-dependent protein kinase specific activity.
W. J. Perkins, D. O. Warner, and K. A. Jones (2009)
Am J Physiol Lung Cell Mol Physiol 296, L121-L129
   Abstract »    Full Text »    PDF »
Maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared with adult ovine carotid arteries.
E. R. Injeti, R. J. Sandoval, J. M. Williams, A. V. Smolensky, L. E. Ford, and W. J. Pearce (2008)
Am J Physiol Heart Circ Physiol 295, H2289-H2298
   Abstract »    Full Text »    PDF »
Probing the Interaction between the Coiled Coil Leucine Zipper of cGMP-dependent Protein Kinase I{alpha} and the C Terminus of the Myosin Binding Subunit of the Myosin Light Chain Phosphatase.
A. K. Sharma, G.-P. Zhou, J. Kupferman, H. K. Surks, E. N. Christensen, J. J. Chou, M. E. Mendelsohn, and A. C. Rigby (2008)
J. Biol. Chem. 283, 32860-32869
   Abstract »    Full Text »    PDF »
Preservation of cGMP-induced relaxation of pulmonary veins of fetal lambs exposed to chronic high altitude hypoxia: role of PKG and Rho kinase.
Y. Gao, A. D. Portugal, J. Liu, S. Negash, W. Zhou, J. Tian, R. Xiang, L. D. Longo, and J. U. Raj (2008)
Am J Physiol Lung Cell Mol Physiol 295, L889-L896
   Abstract »    Full Text »    PDF »
High blood pressure arising from a defect in vascular function.
S. K. Michael, H. K. Surks, Y. Wang, Y. Zhu, R. Blanton, M. Jamnongjit, M. Aronovitz, W. Baur, K. Ohtani, M. K. Wilkerson, et al. (2008)
PNAS 105, 6702-6707
   Abstract »    Full Text »    PDF »
Uterine artery myosin phosphatase isoform switching and increased sensitivity to SNP in a rat L-NAME model of hypertension of pregnancy.
Y. Lu, H. Zhang, N. Gokina, M. Mandala, O. Sato, M. Ikebe, G. Osol, and S. A. Fisher (2008)
Am J Physiol Cell Physiol 294, C564-C571
   Abstract »    Full Text »    PDF »
The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation.
P. Komalavilas, R. B. Penn, C. R. Flynn, J. Thresher, L. B. Lopes, E. J. Furnish, M. Guo, M. A. Pallero, J. E. Murphy-Ullrich, and C. M. Brophy (2008)
Am J Physiol Lung Cell Mol Physiol 294, L69-L78
   Abstract »    Full Text »    PDF »
Association of VASP with TRPC4 in PKG-mediated inhibition of the store-operated calcium response in mesangial cells.
X. Wang, J. L. Pluznick, D. C. Settles, and S. C. Sansom (2007)
Am J Physiol Renal Physiol 293, F1768-F1776
   Abstract »    Full Text »    PDF »
cGMP-Dependent Protein Kinase I and Smooth Muscle Relaxation: A Tale of Two Isoforms.
H. K. Surks (2007)
Circ. Res. 101, 1078-1080
   Full Text »    PDF »
Phosphodiesterase Type 5: Expanding Roles in Cardiovascular Regulation.
D. A. Kass, H. C. Champion, and J. A. Beavo (2007)
Circ. Res. 101, 1084-1095
   Abstract »    Full Text »    PDF »
Rescue of cGMP Kinase I Knockout Mice by Smooth Muscle Specific Expression of Either Isozyme.
S. Weber, D. Bernhard, R. Lukowski, P. Weinmeister, R. Worner, J. W. Wegener, N. Valtcheva, S. Feil, J. Schlossmann, F. Hofmann, et al. (2007)
Circ. Res. 101, 1096-1103
   Abstract »    Full Text »    PDF »
A Cysteine-rich LIM-only Protein Mediates Regulation of Smooth Muscle-specific Gene Expression by cGMP-dependent Protein Kinase.
T. Zhang, S. Zhuang, D. E. Casteel, D. J. Looney, G. R. Boss, and R. B. Pilz (2007)
J. Biol. Chem. 282, 33367-33380
   Abstract »    Full Text »    PDF »
The receptor guanylyl cyclase Npr2 is essential for sensory axon bifurcation within the spinal cord.
H. Schmidt, A. Stonkute, R. Juttner, S. Schaffer, J. Buttgereit, R. Feil, F. Hofmann, and F. G. Rathjen (2007)
J. Cell Biol. 179, 331-340
   Abstract »    Full Text »    PDF »
cGMP-dependent protein kinase I interacts with TRIM39R, a novel Rpp21 domain-containing TRIM protein.
J. D. Roberts Jr., J.-D. Chiche, E. M. Kolpa, D. B. Bloch, and K. D. Bloch (2007)
Am J Physiol Lung Cell Mol Physiol 293, L903-L912
   Abstract »    Full Text »    PDF »
Cyclic GMP Regulation of Myosin Phosphatase: A New Piece for the Puzzle?.
A. V. Somlyo (2007)
Circ. Res. 101, 645-647
   Full Text »    PDF »
Central Role of Calcium-Dependent Tyrosine Kinase PYK2 in Endothelial Nitric Oxide Synthase Mediated Angiogenic Response and Vascular Function.
A. Matsui, M. Okigaki, K. Amano, Y. Adachi, D. Jin, S. Takai, T. Yamashita, S. Kawashima, T. Kurihara, M. Miyazaki, et al. (2007)
Circulation 116, 1041-1051
   Abstract »    Full Text »    PDF »
Cardiovascular Actions of Insulin.
R. Muniyappa, M. Montagnani, K. K. Koh, and M. J. Quon (2007)
Endocr. Rev. 28, 463-491
   Abstract »    Full Text »    PDF »
Reduction in soluble guanylyl cyclase-specific activity following prolonged treatment of porcine pulmonary artery with nitric oxide.
W. J. Perkins, M. Taniguchi, D. O. Warner, E. N. Chini, and K. A. Jones (2007)
Am J Physiol Lung Cell Mol Physiol 293, L84-L95
   Abstract »    Full Text »    PDF »
Conditioning Effect of Blood Flow on Resistance Artery Smooth Muscle Myosin Phosphatase.
H. Zhang and S. A. Fisher (2007)
Circ. Res. 100, 730-737
   Abstract »    Full Text »    PDF »
Role of Rho kinases in PKG-mediated relaxation of pulmonary arteries of fetal lambs exposed to chronic high altitude hypoxia.
Y. Gao, A. D. Portugal, S. Negash, W. Zhou, L. D. Longo, and J. Usha Raj (2007)
Am J Physiol Lung Cell Mol Physiol 292, L678-L684
   Abstract »    Full Text »    PDF »
ROCK1 Phosphorylates and Activates Zipper-interacting Protein Kinase.
L. Hagerty, D. H. Weitzel, J. Chambers, C. N. Fortner, M. H. Brush, D. Loiselle, H. Hosoya, and T. A. J. Haystead (2007)
J. Biol. Chem. 282, 4884-4893
   Abstract »    Full Text »    PDF »
Ca2+-Independent, Inhibitory Effects of Cyclic Adenosine 5'-Monophosphate on Ca2+ Regulation of Phosphoinositide 3-Kinase C2{alpha}, Rho, and Myosin Phosphatase in Vascular Smooth Muscle.
M. A. Azam, K. Yoshioka, S. Ohkura, N. Takuwa, N. Sugimoto, K. Sato, and Y. Takuwa (2007)
J. Pharmacol. Exp. Ther. 320, 907-916
   Abstract »    Full Text »    PDF »
Myosin Phosphatase Regulatory Pathways: Different Functions or Redundant Functions?.
T. M. Lincoln (2007)
Circ. Res. 100, 10-12
   Full Text »    PDF »
Hyperoxic conditions inhibit airway smooth muscle myosin phosphatase in rat pups.
P. G. Smith, A. Dreshaj, S. Chaudhuri, B. M. Onder, M. J. Mhanna, and R. J. Martin (2007)
Am J Physiol Lung Cell Mol Physiol 292, L68-L73
   Abstract »    Full Text »    PDF »
MYPT1 mutants demonstrate the importance of aa 888-928 for the interaction with PKGI{alpha}.
A. M. Given, O. Ogut, and F. V. Brozovich (2007)
Am J Physiol Cell Physiol 292, C432-C439
   Abstract »    Full Text »    PDF »
cGMP signals mainly through cAMP kinase in permeabilized murine aorta.
R. Worner, R. Lukowski, F. Hofmann, and J. W. Wegener (2007)
Am J Physiol Heart Circ Physiol 292, H237-H244
   Abstract »    Full Text »    PDF »
AKT phosphorylation is essential for insulin-induced relaxation of rat vascular smooth muscle cells.
J. H. Lee and L. Ragolia (2006)
Am J Physiol Cell Physiol 291, C1355-C1365
   Abstract »    Full Text »    PDF »
cGMP-dependent Protein Kinase Type I Inhibits TAB1-p38 Mitogen-activated Protein Kinase Apoptosis Signaling in Cardiac Myocytes.
B. Fiedler, R. Feil, F. Hofmann, C. Willenbockel, H. Drexler, A. Smolenski, S. M. Lohmann, and K. C. Wollert (2006)
J. Biol. Chem. 281, 32831-32840
   Abstract »    Full Text »    PDF »
Phosphorylation of Myosin Phosphatase Targeting Subunit 3 (MYPT3) and Regulation of Protein Phosphatase 1 by Protein Kinase A.
J. Yong, I. Tan, L. Lim, and T. Leung (2006)
J. Biol. Chem. 281, 31202-31211
   Abstract »    Full Text »    PDF »
Convergence of Ca2+-desensitizing mechanisms activated by forskolin and phenylephrine pretreatment, but not 8-bromo-cGMP.
M. Porter, M. C. Evans, A. S. Miner, K. M. Berg, K. R. Ward, and P. H. Ratz (2006)
Am J Physiol Cell Physiol 290, C1552-C1559
   Abstract »    Full Text »    PDF »
A Novel Gain-of-Function Mutant of the Cyclic GMP-Dependent Protein Kinase egl-4 Affects Multiple Physiological Processes in Caenorhabditis elegans.
D. M. Raizen, K. M. Cullison, A. I. Pack, and M. V. Sundaram (2006)
Genetics 173, 177-187
   Abstract »    Full Text »    PDF »
Cyclic GMP-dependent Protein Kinase I{alpha} Inhibits Thrombin Receptor-mediated Calcium Mobilization in Vascular Smooth Muscle Cells.
E. N. Christensen and M. E. Mendelsohn (2006)
J. Biol. Chem. 281, 8409-8416
   Abstract »    Full Text »    PDF »
Nitric oxide synthase localization in the rat neutrophils: immunocytochemical, molecular, and biochemical studies.
R. Saini, S. Patel, R. Saluja, A. A. Sahasrabuddhe, M. P. Singh, S. Habib, V. K. Bajpai, and M. Dikshit (2006)
J. Leukoc. Biol. 79, 519-528
   Abstract »    Full Text »    PDF »
Nitric oxide and cGMP protein kinase (cGK) regulate dendritic-cell migration toward the lymph-node-directing chemokine CCL19.
D. Giordano, D. M. Magaletti, and E. A. Clark (2006)
Blood 107, 1537-1545
   Abstract »    Full Text »    PDF »
Smooth muscle of telokin-deficient mice exhibits increased sensitivity to Ca2+ and decreased cGMP-induced relaxation.
A. S. Khromov, H. Wang, N. Choudhury, M. McDuffie, B. P. Herring, R. Nakamoto, G. K. Owens, A. P. Somlyo, and A. V. Somlyo (2006)
PNAS 103, 2440-2445
   Abstract »    Full Text »    PDF »
Function of cGMP-Dependent Protein Kinases as Revealed by Gene Deletion.
F. Hofmann, R. Feil, T. Kleppisch, and J. Schlossmann (2006)
Physiol Rev 86, 1-23
   Abstract »    Full Text »    PDF »
M-RIP Targets Myosin Phosphatase to Stress Fibers to Regulate Myosin Light Chain Phosphorylation in Vascular Smooth Muscle Cells.
H. K. Surks, N. Riddick, and K.-i. Ohtani (2005)
J. Biol. Chem. 280, 42543-42551
   Abstract »    Full Text »    PDF »
Identification of the Interface between cGMP-dependent Protein Kinase I{beta} and Its Interaction Partners TFII-I and IRAG Reveals a Common Interaction Motif.
D. E. Casteel, G. R. Boss, and R. B. Pilz (2005)
J. Biol. Chem. 280, 38211-38218
   Abstract »    Full Text »    PDF »
cGMP Inhibition of Na+/H+ Antiporter 3 (NHE3) Requires PDZ Domain Adapter NHERF2, a Broad Specificity Protein Kinase G-anchoring Protein.
B. Cha, J. H. Kim, H. Hut, B. M. Hogema, J. Nadarja, M. Zizak, M. Cavet, W. Lee-Kwon, S. M. Lohmann, A. Smolenski, et al. (2005)
J. Biol. Chem. 280, 16642-16650
   Abstract »    Full Text »    PDF »
The Biology of Cyclic GMP-dependent Protein Kinases.
F. Hofmann (2005)
J. Biol. Chem. 280, 1-4
   Full Text »    PDF »
Striatin assembles a membrane signaling complex necessary for rapid, nongenomic activation of endothelial NO synthase by estrogen receptor {alpha}.
Q. Lu, D. C. Pallas, H. K. Surks, W. E. Baur, M. E. Mendelsohn, and R. H. Karas (2004)
PNAS 101, 17126-17131
   Abstract »    Full Text »    PDF »
p116Rip Targets Myosin Phosphatase to the Actin Cytoskeleton and Is Essential for RhoA/ROCK-regulated Neuritogenesis.
J. Mulder, A. Ariaens, D. van den Boomen, and W. H. Moolenaar (2004)
Mol. Biol. Cell 15, 5516-5527
   Abstract »    Full Text »    PDF »
cGMP-Dependent Protein Kinase Mediates NO- but not Acetylcholine-Induced Dilations in Resistance Vessels In Vivo.
M. Koeppen, R. Feil, D. Siegl, S. Feil, F. Hofmann, U. Pohl, and C. de Wit (2004)
Hypertension 44, 952-955
   Abstract »    Full Text »    PDF »
Identification and Characterization of Zipper-interacting Protein Kinase as the Unique Vascular Smooth Muscle Myosin Phosphatase-associated Kinase.
A. Endo, H. K. Surks, S. Mochizuki, N. Mochizuki, and M. E. Mendelsohn (2004)
J. Biol. Chem. 279, 42055-42061
   Abstract »    Full Text »    PDF »
Downregulation of cGMP-dependent protein kinase-1 activity in the corpus cavernosum smooth muscle of diabetic rabbits.
S. Chang, J. A. Hypolite, M. Velez, A. Changolkar, A. J. Wein, S. Chacko, and M. E. DiSanto (2004)
Am J Physiol Regulatory Integrative Comp Physiol 287, R950-R960
   Abstract »    Full Text »    PDF »
Vascular Reactivity in Heart Failure: Role of Myosin Light Chain Phosphatase.
S. M. Karim, A. Y. Rhee, A. M. Given, M. D. Faulx, B. D. Hoit, and F. V. Brozovich (2004)
Circ. Res. 95, 612-618
   Abstract »    Full Text »    PDF »
Role of Protein Phosphatase Type 1 in Contractile Functions: Myosin Phosphatase.
D. J. Hartshorne, M. Ito, and F. Erdodi (2004)
J. Biol. Chem. 279, 37211-37214
   Full Text »    PDF »
Smooth Muscle Phosphatase Is Regulated in Vivo by Exclusion of Phosphorylation of Threonine 696 of MYPT1 by Phosphorylation of Serine 695 in Response to Cyclic Nucleotides.
A. A. Wooldridge, J. A. MacDonald, F. Erdodi, C. Ma, M. A. Borman, D. J. Hartshorne, and T. A. J. Haystead (2004)
J. Biol. Chem. 279, 34496-34504
   Abstract »    Full Text »    PDF »
Actions Downstream of Cyclic GMP/Protein Kinase G Can Reverse Protein Kinase C-mediated Phosphorylation of CPI-17 and Ca2+Sensitization in Smooth Muscle.
J. Bonnevier and A. Arner (2004)
J. Biol. Chem. 279, 28998-29003
   Abstract »    Full Text »    PDF »
Downregulation of cGMP-dependent protein kinase expression by inflammatory cytokines in vascular smooth muscle cells.
N. C. Browner, H. Sellak, and T. M. Lincoln (2004)
Am J Physiol Cell Physiol 287, C88-C96
   Abstract »    Full Text »    PDF »
Formin Homology Domain Protein (FHOD1) Is a Cyclic GMP-dependent Protein Kinase I-binding Protein and Substrate in Vascular Smooth Muscle Cells.
Y. Wang, M. R. El-Zaru, H. K. Surks, and M. E. Mendelsohn (2004)
J. Biol. Chem. 279, 24420-24426
   Abstract »    Full Text »    PDF »
Regulation of Uterine Function: a Biochemical Conundrum in the Regulation of Smooth Muscle Relaxation.
I. L. O. Buxton (2004)
Mol. Pharmacol. 65, 1051-1059
   Abstract »    Full Text »
Inhibition of cGMP-Dependent Protein Kinase by the Cell-Permeable Peptide DT-2 Reveals a Novel Mechanism of Vasoregulation.
M. S. Taylor, C. Okwuchukwuasanya, C. K. Nickl, W. Tegge, J. E. Brayden, and W. R. G. Dostmann (2004)
Mol. Pharmacol. 65, 1111-1119
   Abstract »    Full Text »
Insulin resistance and hypertension.
J. R. Sowers (2004)
Am J Physiol Heart Circ Physiol 286, H1597-H1602
   Abstract »    Full Text »    PDF »
Dynamic changes in expression of myosin phosphatase in a model of portal hypertension.
M. C. Payne, H.-Y. Zhang, Y. Shirasawa, Y. Koga, M. Ikebe, J. N. Benoit, and S. A. Fisher (2004)
Am J Physiol Heart Circ Physiol 286, H1801-H1810
   Abstract »    Full Text »    PDF »
Intraluminal Pressure Is a Stimulus for NFATc3 Nuclear Accumulation: ROLE OF CALCIUM, ENDOTHELIUM-DERIVED NITRIC OXIDE, AND cGMP-DEPENDENT PROTEIN KINASE.
L. V. Gonzalez Bosc, M. K. Wilkerson, K. N. Bradley, D. M. Eckman, D. C. Hill-Eubanks, and M. T. Nelson (2004)
J. Biol. Chem. 279, 10702-10709
   Abstract »    Full Text »    PDF »
Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts.
G. Totsukawa, Y. Wu, Y. Sasaki, D. J. Hartshorne, Y. Yamakita, S. Yamashiro, and F. Matsumura (2004)
J. Cell Biol. 164, 427-439
   Abstract »    Full Text »    PDF »
Unzipping the Role of Myosin Light Chain Phosphatase in Smooth Muscle Cell Relaxation.
Q. Q. Huang, S. A. Fisher, and F. V. Brozovich (2004)
J. Biol. Chem. 279, 597-603
   Abstract »    Full Text »    PDF »
Functional Diversity of Protein Phosphatase-1, a Cellular Economizer and Reset Button.
H. CEULEMANS and M. BOLLEN (2004)
Physiol Rev 84, 1-39
   Abstract »    Full Text »    PDF »
C-type natriuretic peptide induces human colonic myofibroblast relaxation.
T. Chitapanarux, S. L. Chen, H. Lee, A. C. Melton, and H. F. Yee Jr. (2004)
Am J Physiol Gastrointest Liver Physiol 286, G31-G36
   Abstract »    Full Text »    PDF »
The {alpha}, but Not the {beta}, Isoform of the Human Thromboxane A2 Receptor Is a Target for Nitric Oxide-mediated Desensitization: INDEPENDENT MODULATION OF TP{alpha} SIGNALING BY NITRIC OXIDE AND PROSTACYCLIN.
H. M. Reid and B. T. Kinsella (2003)
J. Biol. Chem. 278, 51190-51202
   Abstract »    Full Text »    PDF »
Myosin Phosphatase-Rho Interacting Protein: A NEW MEMBER OF THE MYOSIN PHOSPHATASE COMPLEX THAT DIRECTLY BINDS RhoA.
H. K. Surks, C. T. Richards, and M. E. Mendelsohn (2003)
J. Biol. Chem. 278, 51484-51493
   Abstract »    Full Text »    PDF »
Dimerization of cGMP-dependent Protein Kinase I{beta} Is Mediated by an Extensive Amino-terminal Leucine Zipper Motif, and Dimerization Modulates Enzyme Function.
R. Richie-Jannetta, S. H. Francis, and J. D. Corbin (2003)
J. Biol. Chem. 278, 50070-50079
   Abstract »    Full Text »    PDF »
Poplar Metal Tolerance Protein 1 Confers Zinc Tolerance and Is an Oligomeric Vacuolar Zinc Transporter with an Essential Leucine Zipper Motif.
D. Blaudez, A. Kohler, F. Martin, D. Sanders, and M. Chalot (2003)
PLANT CELL 15, 2911-2928
   Abstract »    Full Text »    PDF »
Cyclic GMP-Dependent Protein Kinases and the Cardiovascular System: Insights From Genetically Modified Mice.
R. Feil, S. M. Lohmann, H. de Jonge, U. Walter, and F. Hofmann (2003)
Circ. Res. 93, 907-916
   Abstract »    Full Text »    PDF »
Physiology and Pathophysiology of Vascular Signaling Controlled by Cyclic Guanosine 3',5'-Cyclic Monophosphate-Dependent Protein Kinase.
T. Munzel, R. Feil, A. Mulsch, S. M. Lohmann, F. Hofmann, and U. Walter (2003)
Circulation 108, 2172-2183
   Full Text »    PDF »
PDZ Domain-mediated Interaction of Interleukin-16 Precursor Proteins with Myosin Phosphatase Targeting Subunits.
N. Bannert, K. Vollhardt, B. Asomuddinov, M. Haag, H. Konig, S. Norley, and R. Kurth (2003)
J. Biol. Chem. 278, 42190-42199
   Abstract »    Full Text »    PDF »
Ca2+ Sensitivity of Smooth Muscle and Nonmuscle Myosin II: Modulated by G Proteins, Kinases, and Myosin Phosphatase.
A. P. SOMLYO and A. V. SOMLYO (2003)
Physiol Rev 83, 1325-1358
   Abstract »    Full Text »    PDF »
Hypoxia Inhibits Myosin Phosphatase in Pulmonary Arterial Smooth Muscle Cells: Role of Rho-Kinase.
Z. Wang, M. C. Lanner, N. Jin, D. Swartz, L. Li, and R. A. Rhoades (2003)
Am. J. Respir. Cell Mol. Biol. 29, 465-471
   Abstract »    Full Text »    PDF »
Differential relaxing responses to particulate or soluble guanylyl cyclase activation on endothelial cells: a mechanism dependent on PKG-I{alpha} activation by NO/cGMP.
F. J. Rivero-Vilches, S De Frutos, M Saura, D Rodriguez-Puyol, and M Rodriguez-Puyol (2003)
Am J Physiol Cell Physiol 285, C891-C898
   Abstract »    Full Text »    PDF »
Cyclic GMP Phosphodiesterases and Regulation of Smooth Muscle Function.
S. D. Rybalkin, C. Yan, K. E. Bornfeldt, and J. A. Beavo (2003)
Circ. Res. 93, 280-291
   Abstract »    Full Text »    PDF »
Invited Review: Do inflammatory mediators influence the contribution of airway smooth muscle contraction to airway hyperresponsiveness in asthma?.
D. J. Fernandes, R. W. Mitchell, O. Lakser, M. Dowell, A. G. Stewart, and J. Solway (2003)
J Appl Physiol 95, 844-853
   Abstract »    Full Text »    PDF »
Hypoxia enhances a cGMP-independent nitric oxide relaxing mechanism in pulmonary arteries.
C. J. Mingone, S. A. Gupte, T. Iesaki, and M. S. Wolin (2003)
Am J Physiol Lung Cell Mol Physiol 285, L296-L304
   Abstract »    Full Text »    PDF »
Nitric Oxide-Induced Decrease in Calcium Sensitivity of Resistance Arteries Is Attributable to Activation of the Myosin Light Chain Phosphatase and Antagonized by the RhoA/Rho Kinase Pathway.
S.-S. Bolz, L. Vogel, D. Sollinger, R. Derwand, C. de Wit, G. Loirand, and U. Pohl (2003)
Circulation 107, 3081-3087
   Abstract »    Full Text »    PDF »
Inhibition of sustained smooth muscle contraction by PKA and PKG preferentially mediated by phosphorylation of RhoA.
K. S. Murthy, H. Zhou, J. R. Grider, and G. M. Makhlouf (2003)
Am J Physiol Gastrointest Liver Physiol 284, G1006-G1016
   Abstract »    Full Text »    PDF »
ERK and RhoA Differentially Regulate Pseudopodia Growth and Retraction during Chemotaxis.
A. A. Brahmbhatt and R. L. Klemke (2003)
J. Biol. Chem. 278, 13016-13025
   Abstract »    Full Text »    PDF »
Regulation of Estrogen Receptor alpha -mediated Transcription by a Direct Interaction with Protein Phosphatase 2A.
Q. Lu, H. K. Surks, H. Ebling, W. E. Baur, D. Brown, D. C. Pallas, and R. H. Karas (2003)
J. Biol. Chem. 278, 4639-4645
   Abstract »    Full Text »    PDF »
Selective phosphorylation of the IP3R-I in vivo by cGMP-dependent protein kinase in smooth muscle.
K. S. Murthy and H. Zhou (2003)
Am J Physiol Gastrointest Liver Physiol 284, G221-G230
   Abstract »    Full Text »    PDF »
Quinine Inhibits Vascular Contraction Independent of Effects on Calcium or Myosin Phosphorylation.
B. Adegunloye, E. Lamarre, and R. S. Moreland (2003)
J. Pharmacol. Exp. Ther. 304, 294-300
   Abstract »    Full Text »    PDF »
Functional Interplay Between Angiotensin II and Nitric Oxide: Cyclic GMP as a Key Mediator.
C. Yan, D. Kim, T. Aizawa, and B. C. Berk (2003)
Arterioscler Thromb Vasc Biol 23, 26-36
   Abstract »    Full Text »    PDF »
cGMP-mediated signaling via cGKI{alpha} is required for the guidance and connectivity of sensory axons.
H. Schmidt, M. Werner, P. A. Heppenstall, M. Henning, M. I. More, S. Kuhbandner, G. R. Lewin, F. Hofmann, R. Feil, and F. G. Rathjen (2002)
J. Cell Biol. 159, 489-498
   Abstract »    Full Text »    PDF »
Activation of the Mitogen Activated Protein Kinase Extracellular Signal-Regulated Kinase 1 and 2 by the Nitric Oxide-cGMP-cGMP-Dependent Protein Kinase Axis Regulates the Expression of Matrix Metalloproteinase 13 in Vascular Endothelial Cells.
C. Zaragoza, E. Soria, E. Lopez, D. Browning, M. Balbin, C. Lopez-Otin, and S. Lamas (2002)
Mol. Pharmacol. 62, 927-935
   Abstract »    Full Text »    PDF »
Nitric Oxide-Induced Motility in Aortic Smooth Muscle Cells: Role of Protein Tyrosine Phosphatase SHP-2 and GTP-Binding Protein Rho.
Y. Chang, B. Ceacareanu, M. Dixit, N. Sreejayan, and A. Hassid (2002)
Circ. Res. 91, 390-397
   Abstract »    Full Text »    PDF »
cGMP-dependent Protein Kinase Ibeta Physically and Functionally Interacts with the Transcriptional Regulator TFII-I.
D. E. Casteel, S. Zhuang, T. Gudi, J. Tang, M. Vuica, S. Desiderio, and R. B. Pilz (2002)
J. Biol. Chem. 277, 32003-32014
   Abstract »    Full Text »    PDF »
Regulation of Structural Plasticity by Different Channel Types in Rod and Cone Photoreceptors.
N. Zhang and E. Townes-Anderson (2002)
J. Neurosci. 22, 7065-7079
   Abstract »    Full Text »    PDF »
The pathophysiology of erectile dysfunction related to endothelial dysfunction and mediators of vascular function.
R. Maas, E. Schwedhelm, J. Albsmeier, and R. H Boger (2002)
Vascular Medicine 7, 213-225
   Abstract »    PDF »
Functional Reconstitution of Vascular Smooth Muscle Cells With cGMP-Dependent Protein Kinase I Isoforms.
R. Feil, N. Gappa, M. Rutz, J. Schlossmann, C. R. Rose, A. Konnerth, S. Brummer, S. Kuhbandner, and F. Hofmann (2002)
Circ. Res. 90, 1080-1086
   Abstract »    Full Text »    PDF »
Active Rho Kinase (ROK-alpha ) Associates with Insulin Receptor Substrate-1 and Inhibits Insulin Signaling in Vascular Smooth Muscle Cells.
N. Begum, O. A. Sandu, M. Ito, S. M. Lohmann, and A. Smolenski (2002)
J. Biol. Chem. 277, 6214-6222
   Abstract »    Full Text »    PDF »
A Novel Leucine Zipper Targets AKAP15 and Cyclic AMP-dependent Protein Kinase to the C Terminus of the Skeletal Muscle Ca2+ Channel and Modulates Its Function.
J. T. Hulme, M. Ahn, S. D. Hauschka, T. Scheuer, and W. A. Catterall (2002)
J. Biol. Chem. 277, 4079-4087
   Abstract »    Full Text »    PDF »
Regulation of cGMP-specific Phosphodiesterase (PDE5) Phosphorylation in Smooth Muscle Cells.
S. D. Rybalkin, I. G. Rybalkina, R. Feil, F. Hofmann, and J. A. Beavo (2002)
J. Biol. Chem. 277, 3310-3317
   Abstract »    Full Text »    PDF »
Gene Transfer of cGMP-Dependent Protein Kinase I Enhances the Antihypertrophic Effects of Nitric Oxide in Cardiomyocytes.
K. C. Wollert, B. Fiedler, S. Gambaryan, A. Smolenski, J. Heineke, E. Butt, C. Trautwein, S. M. Lohmann, and H. Drexler (2002)
Hypertension 39, 87-92
   Abstract »    Full Text »    PDF »
cAMP inhibits IP3-dependent Ca2+ release by preferential activation of cGMP-primed PKG.
K. S. Murthy (2001)
Am J Physiol Gastrointest Liver Physiol 281, G1238-G1245
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)