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Science 12 November 1999:
Vol. 286. no. 5443, pp. 1371 - 1374
DOI: 10.1126/science.286.5443.1371
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Reports

Evidence for Autoregulation of Cystathionine gamma -Synthase mRNA Stability in Arabidopsis

Yukako Chiba, 1 Mari Ishikawa, 1 Fumiko Kijima, 1* R. Huw Tyson, 2 Jungsup Kim, 3 Ayako Yamamoto, 1dagger Eiji Nambara, 1 Thomas Leustek, 3 Roger M. Wallsgrove, 2 Satoshi Naito 1ddagger

Control of messenger RNA (mRNA) stability serves as an important mechanism for regulating gene expression. Analysis of Arabidopsis mutants that overaccumulate soluble methionine (Met) revealed that the gene for cystathionine gamma -synthase (CGS), the key enzyme in Met biosynthesis, is regulated at the level of mRNA stability. Transfection experiments with wild-type and mutant forms of the CGS gene suggest that an amino acid sequence encoded by the first exon of CGS acts in cis to destabilize its own mRNA in a process that is activated by Met or one of its metabolites.

1 Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan.
2 Biochemistry and Physiology Department, IACR-Rothamsted, Harpenden, Hartfordshire AL5 2JQ, United Kingdom.
3 Biotechnology Center for Agriculture and the Environment, Rutgers University, New Brunswick, NJ 08901-8520, USA.
*   Present address: Fukujuen CHA Research Center, Kyoto 619-0223, Japan.

dagger    Present address: Laboratory of Molecular Phylogenetics, Institute of Molecular and Cellular Biosciences, The University of Tokyo, Tokyo 113-0032, Japan.

ddagger    To whom correspondence should be addressed. E-mail: naito{at}abs.agr.hokudai.ac.jp

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