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Science 12 November 1999:
Vol. 286. no. 5443, pp. 1365 - 1367
DOI: 10.1126/science.286.5443.1365
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Reports

Induction of Metaphase Arrest in Cleaving Xenopus Embryos by the Protein Kinase p90Rsk

Stefan D. Gross, Markus S. Schwab, Andrea L. Lewellyn, James L. Maller *

Before fertilization, vertebrate eggs are arrested in metaphase of meiosis II by cytostatic factor (CSF), an activity that requires activation of the mitogen-activated protein kinase (MAPK) pathway. To investigate whether CSF arrest is mediated by the protein kinase p90Rsk, which is phosphorylated and activated by MAPK, a constitutively activated (CA) form of Rsk was expressed in Xenopus embryos. Expression of CA Rsk resulted in cleavage arrest, and cytological analysis showed that arrested blastomeres were in M phase with prominent spindles characteristic of meiotic metaphase. Thus, Rsk appears to be the mediator of MAPK-dependent CSF arrest in vertebrate unfertilized eggs.

Howard Hughes Medical Institute and Department of Pharmacology, University of Colorado School of Medicine, Denver, CO 80262, USA.
*   To whom correspondence should be addressed. E-mail: jim.maller{at}uchsc.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)