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Science 5 November 1999:
Vol. 286. no. 5442, pp. 1153 - 1155
DOI: 10.1126/science.286.5442.1153
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Reports

A Biochemical Genomics Approach for Identifying Genes by the Activity of Their Products

Mark R. Martzen, 1 Stephen M. McCraith, 2 Sherry L. Spinelli, 1 Francy M. Torres, 1 Stanley Fields, 23 Elizabeth J. Grayhack, 1 Eric M. Phizicky 1*

For the identification of yeast genes specifying biochemical activities, a genomic strategy that is rapid, sensitive, and widely applicable was developed with an array of 6144 individual yeast strains, each containing a different yeast open reading frame (ORF) fused to glutathione S-transferase (GST). For the identification of ORF-associated activities, strains were grown in defined pools, and GST-ORFs were purified. Then, pools were assayed for activities, and active pools were deconvoluted to identify the source strains. Three previously unknown ORF-associated activities were identified with this strategy: a cyclic phosphodiesterase that acts on adenosine diphosphate-ribose 1"-2" cyclic phosphate (Appr>p), an Appr-1"-p-processing activity, and a cytochrome c methyltransferase.

1 Department of Biochemistry and Biophysics, University of Rochester School of Medicine, Rochester, NY 14642, USA.
2 Departments of Genetics and Medicine,
3 Howard Hughes Medical Institute, University of Washington, Seattle, WA 98195, USA.
*   To whom correspondence should be addressed. E-mail: eric_phizicky{at}urmc.rochester.edu

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   Abstract »    Full Text »
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   Abstract »    Full Text »
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   Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
Cytochrome c Methyltransferase, Ctm1p, of Yeast.
B. Polevoda, M. R. Martzen, B. Das, E. M. Phizicky, and F. Sherman (2000)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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High-Density Cell Microarrays for Parallel Functional Determinations.
C. W. Xu (2002)
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