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Science 22 October 1999:
Vol. 286. no. 5440, pp. 766 - 768
DOI: 10.1126/science.286.5440.766
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Reports

Interlocked Feedback Loops Within the Drosophila Circadian Oscillator

Nick R. J. Glossop, * Lisa C. Lyons, * Paul E. Hardin dagger

Drosophila Clock (dClk) is rhythmically expressed, with peaks in mRNA and protein (dCLK) abundance early in the morning. dClk mRNA cycling is shown here to be regulated by PERIOD-TIMELESS (PER-TIM)-mediated release of dCLK- and CYCLE (CYC)-dependent repression. Lack of both PER-TIM derepression and dCLK-CYC repression results in high levels of dClk mRNA, which implies that a separate dClk activator is present. These results demonstrate that the Drosophila circadian feedback loop is composed of two interlocked negative feedback loops: a per-tim loop, which is activated by dCLK-CYC and repressed by PER-TIM, and a dClk loop, which is repressed by dCLK-CYC and derepressed by PER-TIM.

Department of Biology and Biochemistry and Biological Clocks Program, University of Houston, Houston, TX 77204-5513, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: phardin{at}uh.edu

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