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Science 15 October 1999:
Vol. 286. no. 5439, pp. 537 - 542
DOI: 10.1126/science.286.5439.537
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Reports

Sequencing Complex Polysaccharides

Ganesh Venkataraman, 1 Zachary Shriver, 2 Rahul Raman, 2 Ram Sasisekharan 2*

Although rapid sequencing of polynucleotides and polypeptides has become commonplace, it has not been possible to rapidly sequence femto- to picomole amounts of tissue-derived complex polysaccharides. Heparin-like glycosaminoglycans (HLGAGs) were readily sequenced by a combination of matrix-assisted laser desorption ionization mass spectrometry and a notation system for representation of polysaccharide sequences. This will enable identification of sequences that are critical to HLGAG biological activities in anticoagulation, cell growth, and differentiation.

1 Harvard-MIT Division of Health Sciences and Technology,
2 Division of Bioengineering and Environmental Health, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
*   To whom correspondence should be addressed. E-mail: ramnat{at}mit.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)