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Science 8 October 1999:
Vol. 286. no. 5438, pp. 295 - 299
DOI: 10.1126/science.286.5438.295
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Reports

Evolutionarily Conserved Pathways of Energetic Connectivity in Protein Families

Steve W. Lockless, Rama Ranganathan *

For mapping energetic interactions in proteins, a technique was developed that uses evolutionary data for a protein family to measure statistical interactions between amino acid positions. For the PDZ domain family, this analysis predicted a set of energetically coupled positions for a binding site residue that includes unexpected long-range interactions. Mutational studies confirm these predictions, demonstrating that the statistical energy function is a good indicator of thermodynamic coupling in proteins. Sets of interacting residues form connected pathways through the protein fold that may be the basis for efficient energy conduction within proteins.

Howard Hughes Medical Institute and Department of Pharmacology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75235-9050, USA.
*   To whom correspondence should be addressed. E-mail: rama{at}chop.swmed.edu

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