Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
AAAS Promotion

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 3 September 1999:
Vol. 285. no. 5433, pp. 1576 - 1579
DOI: 10.1126/science.285.5433.1576
Prev | Table of Contents | Next

Reports

Functional Interaction of BRCA1-Associated BARD1 with Polyadenylation Factor CstF-50

Frida E. Kleiman, and James L. Manley *

Polyadenylation of messenger RNA precursors requires a complex protein machinery that is closely integrated with the even more complex transcriptional apparatus. Here a polyadenylation factor, CstF-50 (cleavage stimulation factor), is shown to interact in vitro and in intact cells with a nuclear protein of previously unknown function, BRCA1-associated RING domain protein (BARD1). The BARD1-CstF-50 interaction inhibits polyadenylation in vitro. BARD1, like CstF-50, also interacts with RNA polymerase II. These results indicate that BARD1-mediated inhibition of polyadenylation may prevent inappropriate RNA processing during transcription, perhaps at sites of DNA repair, and they reveal an unanticipated integration of diverse nuclear events.

Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
*   To whom correspondence should be addressed. E-mail: jlm2{at}columbia.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The 3' processing factor CstF functions in the DNA repair response.
N. Mirkin, D. Fonseca, S. Mohammed, M. A. Cevher, J. L. Manley, and F. E. Kleiman (2008)
Nucleic Acids Res. 36, 1792-1804
   Abstract »    Full Text »    PDF »
Oncogenic BARD1 Isoforms Expressed in Gynecological Cancers.
L. Li, S. Ryser, E. Dizin, D. Pils, M. Krainer, C. E. Jefford, F. Bertoni, R. Zeillinger, and I. Irminger-Finger (2007)
Cancer Res. 67, 11876-11885
   Abstract »    Full Text »    PDF »
Nordic collaborative study of the BARD1 Cys557Ser allele in 3956 patients with cancer: enrichment in familial BRCA1/BRCA2 mutation-negative breast cancer but not in other malignancies.
S-M Karppinen, R B Barkardottir, K Backenhorn, T Sydenham, K Syrjakoski, J Schleutker, T Ikonen, K Pylkas, K Rapakko, H Erkko, et al. (2006)
J. Med. Genet. 43, 856-862
   Abstract »    Full Text »    PDF »
Identification and characterization of missense alterations in the BRCA1 associated RING domain (BARD1) gene in breast and ovarian cancer.
M K Sauer and I L Andrulis (2005)
J. Med. Genet. 42, 633-638
   Abstract »    Full Text »    PDF »
Hyperphosphorylation of the BARD1 Tumor Suppressor in Mitotic Cells.
A. D. Choudhury, H. Xu, A. P. Modi, W. Zhang, T. Ludwig, and R. Baer (2005)
J. Biol. Chem. 280, 24669-24679
   Abstract »    Full Text »    PDF »
BRCA1/BARD1 inhibition of mRNA 3' processing involves targeted degradation of RNA polymerase II.
F. E. Kleiman, F. Wu-Baer, D. Fonseca, S. Kaneko, R. Baer, and J. L. Manley (2005)
Genes & Dev. 19, 1227-1237
   Abstract »    Full Text »    PDF »
Conserved and specific functions of mammalian ssu72.
B. St-Pierre, X. Liu, L.-C. T. Kha, X. Zhu, O. Ryan, Z. Jiang, and E. Zacksenhaus (2005)
Nucleic Acids Res. 33, 464-477
   Abstract »    Full Text »    PDF »
Genome-wide mRNA surveillance is coupled to mRNA export.
H. Hieronymus, M. C. Yu, and P. A. Silver (2004)
Genes & Dev. 18, 2652-2662
   Abstract »    Full Text »    PDF »
Mutation screening of the BARD1 gene: evidence for involvement of the Cys557Ser allele in hereditary susceptibility to breast cancer.
S-M Karppinen, K Heikkinen, K Rapakko, and R Winqvist (2004)
J. Med. Genet. 41, e114
   Full Text »    PDF »
Ubiquitination and Proteasomal Degradation of the BRCA1 Tumor Suppressor Is Regulated during Cell Cycle Progression.
A. D. Choudhury, H. Xu, and R. Baer (2004)
J. Biol. Chem. 279, 33909-33918
   Abstract »    Full Text »    PDF »
The prothrombin 3'end formation signal reveals a unique architecture that is sensitive to thrombophilic gain-of-function mutations.
S. Danckwardt, N. H. Gehring, G. Neu-Yilik, P. Hundsdoerfer, M. Pforsich, U. Frede, M. W. Hentze, and A. E. Kulozik (2004)
Blood 104, 428-435
   Abstract »    Full Text »    PDF »
The ankyrin repeat as molecular architecture for protein recognition.
L. K. Mosavi, T. J. Cammett, D. C. Desrosiers, and Z.-y. Peng (2004)
Protein Sci. 13, 1435-1448
   Abstract »    Full Text »    PDF »
BRCA1 Associates with Processive RNA Polymerase II.
S. A. Krum, G. A. Miranda, C. Lin, and T. F. Lane (2003)
J. Biol. Chem. 278, 52012-52020
   Abstract »    Full Text »    PDF »
BARD1 Participates with BRCA1 in Homology-Directed Repair of Chromosome Breaks.
U. K. Westermark, M. Reyngold, A. B. Olshen, R. Baer, M. Jasin, and M. E. Moynahan (2003)
Mol. Cell. Biol. 23, 7926-7936
   Abstract »    Full Text »    PDF »
Loss of Bard1, the Heterodimeric Partner of the Brca1 Tumor Suppressor, Results in Early Embryonic Lethality and Chromosomal Instability.
E. E. McCarthy, J. T. Celebi, R. Baer, and T. Ludwig (2003)
Mol. Cell. Biol. 23, 5056-5063
   Abstract »    Full Text »    PDF »
BRCA1 Augments Transcription by the NF-{kappa}B Transcription Factor by Binding to the Rel Domain of the p65/RelA Subunit.
M. Benezra, N. Chevallier, D. J. Morrison, T. K. MacLachlan, W. S. El-Deiry, and J. D. Licht (2003)
J. Biol. Chem. 278, 26333-26341
   Abstract »    Full Text »    PDF »
Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex.
P. S. Brzovic, J. R. Keeffe, H. Nishikawa, K. Miyamoto, D. Fox III, M. Fukuda, T. Ohta, and R. Klevit (2003)
PNAS 100, 5646-5651
   Abstract »    Full Text »    PDF »
Enhancement of BRCA1 E3 Ubiquitin Ligase Activity through Direct Interaction with the BARD1 Protein.
Y. Xia, G. M. Pao, H.-W. Chen, I. M. Verma, and T. Hunter (2003)
J. Biol. Chem. 278, 5255-5263
   Abstract »    Full Text »    PDF »
Interaction of the EWS NH2 Terminus with BARD1 Links the Ewing's Sarcoma Gene to a Common Tumor Suppressor Pathway.
L. Spahn, R. Petermann, C. Siligan, J. A. Schmid, D. N. T. Aryee, and H. Kovar (2002)
Cancer Res. 62, 4583-4587
   Abstract »    Full Text »    PDF »
BARD1 Induces BRCA1 Intranuclear Foci Formation by Increasing RING-dependent BRCA1 Nuclear Import and Inhibiting BRCA1 Nuclear Export.
M. Fabbro, J. A. Rodriguez, R. Baer, and B. R. Henderson (2002)
J. Biol. Chem. 277, 21315-21324
   Abstract »    Full Text »    PDF »
A Novel Tricomplex of BRCA1, Nmi, and c-Myc Inhibits c-Myc-induced Human Telomerase Reverse Transcriptase Gene (hTERT) Promoter Activity in Breast Cancer.
H. Li, T.-H. Lee, and H. Avraham (2002)
J. Biol. Chem. 277, 20965-20973
   Abstract »    Full Text »    PDF »
Functions of BRCA1 and BRCA2 in the biological response to DNA damage.
A. R. Venkitaraman (2002)
J. Cell Sci. 114, 3591-3598
   Abstract »    Full Text »    PDF »
Redistribution of BRCA1 among Four Different Protein Complexes following Replication Blockage.
N. Chiba and J. D. Parvin (2001)
J. Biol. Chem. 276, 38549-38554
   Abstract »    Full Text »    PDF »
Functional communication between endogenous BRCA1 and its partner, BARD1, during Xenopus laevis development.
V. Joukov, J. Chen, E. A. Fox, J. B. A. Green, and D. M. Livingston (2001)
PNAS
   Abstract »    Full Text »    PDF »
Capping, splicing, and 3' processing are independently stimulated by RNA polymerase II: different functions for different segments of the CTD.
N. Fong and D. L. Bentley (2001)
Genes & Dev. 15, 1783-1795
   Abstract »    Full Text »    PDF »
Identification of an Apoptotic Cleavage Product of BARD1 as an Autoantigen: A Potential Factor in the Antitumoral Response Mediated by Apoptotic Bodies.
F. Gautier, I. Irminger-Finger, M. Grégoire, K. Meflah, and J. Harb (2000)
Cancer Res. 60, 6895-6900
   Abstract »    Full Text »
Polyadenylate Polymerase Enzymatic Activity in Mammary Tumor Cytosols: A New Independent Prognostic Marker in Primary Breast Cancer.
A. Scorilas, M. Talieri, A. Ardavanis, N. Courtis, E. Dimitriadis, J. Yotis, C. M. Tsiapalis, and T. Trangas (2000)
Cancer Res. 60, 5427-5433
   Abstract »    Full Text »
The Drosophila mus101 Gene, Which Links DNA Repair, Replication and Condensation of Heterochromatin in Mitosis, Encodes a Protein With Seven BRCA1 C-Terminus Domains.
R. R. Yamamoto, J. M. Axton, Y. Yamamoto, R. D. C. Saunders, D. M. Glover, and D. S. Henderson (2000)
Genetics 156, 711-721
   Abstract »    Full Text »
HET/SAF-B Overexpression Causes Growth Arrest and Multinuclearity and Is Associated with Aneuploidy in Human Breast Cancer.
S. M. Townson, T. Sullivan, Q. Zhang, G. M. Clark, C. K. Osborne, A. V. Lee, and S. Oesterreich (2000)
Clin. Cancer Res. 6, 3788-3796
   Abstract »    Full Text »
RNA polymerase II and the integration of nuclear events.
Y. Hirose and J. L. Manley (2000)
Genes & Dev. 14, 1415-1429
   Full Text »
Complex Protein Interactions within the Human Polyadenylation Machinery Identify a Novel Component.
Y. Takagaki and J. L. Manley (2000)
Mol. Cell. Biol. 20, 1515-1525
   Abstract »    Full Text »
The RING Heterodimer BRCA1-BARD1 Is a Ubiquitin Ligase Inactivated by a Breast Cancer-derived Mutation.
R. Hashizume, M. Fukuda, I. Maeda, H. Nishikawa, D. Oyake, Y. Yabuki, H. Ogata, and T. Ohta (2001)
J. Biol. Chem. 276, 14537-14540
   Abstract »    Full Text »    PDF »
Repression of BRCA1 through a Feedback Loop Involving p53.
T. K. MacLachlan, B. C. Dash, D. T. Dicker, and W. S. El-Deiry (2000)
J. Biol. Chem. 275, 31869-31875
   Abstract »    Full Text »    PDF »
Nuclear Localization and Cell Cycle-specific Expression of CtIP, a Protein That Associates with the BRCA1 Tumor Suppressor.
X. Yu and R. Baer (2000)
J. Biol. Chem. 275, 18541-18549
   Abstract »    Full Text »    PDF »
BRCA1 interaction with RNA polymerase II reveals a role for hRPB2 and hRPB10alpha in activated transcription.
B. P. Schlegel, V. J. Green, J. A. A. Ladias, and J. D. Parvin (2000)
PNAS 97, 3148-3153
   Abstract »    Full Text »    PDF »
Functional communication between endogenous BRCA1 and its partner, BARD1, during Xenopus laevis development.
V. Joukov, J. Chen, E. A. Fox, J. B. A. Green, and D. M. Livingston (2001)
PNAS 98, 12078-12083
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)