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Science 3 September 1999:
Vol. 285. no. 5433, pp. 1558 - 1562
DOI: 10.1126/science.285.5433.1558
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Reports

Whole-Genome Shotgun Optical Mapping of Deinococcus radiodurans

Jieyi Lin, 1* Rong Qi, 1 Christopher Aston, 1dagger Junping Jing, 1ddagger Thomas S. Anantharaman, 2 Bud Mishra, 2 Owen White, 3 Michael J. Daly, 4 Kenneth W. Minton, 4 J. Craig Venter, 5 David C. Schwartz 12§

A whole-genome restriction map of Deinococcus radiodurans, a radiation-resistant bacterium able to survive up to 15,000 grays of ionizing radiation, was constructed without using DNA libraries, the polymerase chain reaction, or electrophoresis. Very large, randomly sheared, genomic DNA fragments were used to construct maps from individual DNA molecules that were assembled into two circular overlapping maps (2.6 and 0.415 megabases), without gaps. A third smaller chromosome (176 kilobases) was identified and characterized. Aberrant nonlinear DNA structures that may define chromosome structure and organization, as well as intermediates in DNA repair, were directly visualized by optical mapping techniques after gamma  irradiation.

1 W. M. Keck Laboratory for Biomolecular Imaging, Department of Chemistry, New York University, 31 Washington Place, New York, NY 10003, USA.
2 Courant Institute of Mathematical Sciences, Department of Computer Science, New York University, 251 Mercer Street, New York, NY 10012, USA.
3 The Institute for Genomic Research, 9712 Medical Center Drive, Rockville, MD 20850, USA.
4 Uniformed Services University of the Health Sciences, Bethesda, MD 20814, USA.
5 Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA.
*   Present address: Genome Therapeutics Corp., 100 Beaver Street, Waltham, MA 02154, USA.

dagger    Present address: CNS Disorders, Wyeth-Ayerst Research, Princeton, NJ 08543, USA.

ddagger    Present address: Smith Kline Beecham Pharmaceuticals, King of Prussia, PA 19406, USA.

§   To whom correspondence should be addressed. Present address: Departments of Chemistry and Genetics, UW Biotechnology Center, University of Wisconsin, 425 Henry Mall, Madison, WI 53706, USA. E-mail: schwad01{at}mcrcr.med.nyu.edu

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