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Science 20 August 1999:
Vol. 285. no. 5431, pp. 1268 - 1271
DOI: 10.1126/science.285.5431.1268
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Reports

AP-2 Recruitment to Synaptotagmin Stimulated by Tyrosine-Based Endocytic Motifs

Volker Haucke, Pietro De Camilli *

Clathrin-mediated endocytosis is initiated by the recruitment of the clathrin adaptor protein AP-2 to the plasma membrane where the membrane protein synaptotagmin is thought to act as a docking site. AP-2 also interacts with endocytic motifs present in other cargo proteins. Peptides with a tyrosine-based endocytic motif stimulated binding of AP-2 to synaptotagmin and enhanced AP-2 recruitment to the plasma membrane of neuronal and non-neuronal cells. This suggests a mechanism by which nucleation of clathrin-coated pits is stimulated by the loading of cargo proteins.

Department of Cell Biology and Howard Hughes Medical Institute, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06510, USA.
*   To whom correspondence should be addressed. E-mail: pietro.decamilli{at}yale.edu

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