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Calcium-permeable, stretch-activated nonselective cation (SA Cat)
channels mediate cellular responses to mechanical stimuli.However,
genes encoding such channels have not been identifiedin eukaryotes.
The yeast MID1 gene product (Mid1) is requiredfor calcium
influx in the yeast Saccharomyces cerevisiae.
Functionalexpression of Mid1 in Chinese hamster ovary cells conferred
sensitivityto mechanical stress that resulted in increases in both
calciumconductance and the concentration of cytosolic free calcium.
Theseincreases were dependent on the presence of extracellular calciumand were reduced by gadolinium, a blocker of SA Cat channels.Single-channel analyses with cell-attached patches revealed thatMid1
acts as a calcium-permeable, cation-selective stretch-activatedchannel
with a conductance of 32 picosiemens at 150 millimolarcesium chloride
in the pipette. Thus, Mid1 appears to be a eukaryotic,SA Cat channel.
1 Laboratory of Cell Biology, Institute for
Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma
371-8510, Japan.
2 Supermolecular Science Division,
Electrotechnical Laboratory, Tsukuba, Ibaraki 305-8568, Japan.
3 Department of Physiology, Nagoya University School
of Medicine, Nagoya, Aichi 466-8550, Japan.
4 Department of Biology, Tokyo Gakugei University,
Koganei, Tokyo 184-8501, Japan.
*
To whom correspondence should be addressed. E-mail:
iida{at}u-gakugei.ac.jp
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