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Science 6 August 1999:
Vol. 285. no. 5429, pp. 882 - 886
DOI: 10.1126/science.285.5429.882
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Reports

Molecular Identification of a Eukaryotic, Stretch-Activated Nonselective Cation Channel

Makoto Kanzaki, 1 Masahiro Nagasawa, 1 Itaru Kojima, 1 Chikara Sato, 2 Keiji Naruse, 3 Masahiro Sokabe, 3 Hidetoshi Iida 4*

Calcium-permeable, stretch-activated nonselective cation (SA Cat) channels mediate cellular responses to mechanical stimuli. However, genes encoding such channels have not been identified in eukaryotes. The yeast MID1 gene product (Mid1) is required for calcium influx in the yeast Saccharomyces cerevisiae. Functional expression of Mid1 in Chinese hamster ovary cells conferred sensitivity to mechanical stress that resulted in increases in both calcium conductance and the concentration of cytosolic free calcium. These increases were dependent on the presence of extracellular calcium and were reduced by gadolinium, a blocker of SA Cat channels. Single-channel analyses with cell-attached patches revealed that Mid1 acts as a calcium-permeable, cation-selective stretch-activated channel with a conductance of 32 picosiemens at 150 millimolar cesium chloride in the pipette. Thus, Mid1 appears to be a eukaryotic, SA Cat channel.

1 Laboratory of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8510, Japan.
2 Supermolecular Science Division, Electrotechnical Laboratory, Tsukuba, Ibaraki 305-8568, Japan.
3 Department of Physiology, Nagoya University School of Medicine, Nagoya, Aichi 466-8550, Japan.
4 Department of Biology, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan.
*   To whom correspondence should be addressed. E-mail: iida{at}u-gakugei.ac.jp

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