Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 6 August 1999:
Vol. 285. no. 5429, pp. 882 - 886
DOI: 10.1126/science.285.5429.882
Prev | Table of Contents | Next

Reports

Molecular Identification of a Eukaryotic, Stretch-Activated Nonselective Cation Channel

Makoto Kanzaki, 1 Masahiro Nagasawa, 1 Itaru Kojima, 1 Chikara Sato, 2 Keiji Naruse, 3 Masahiro Sokabe, 3 Hidetoshi Iida 4*

Calcium-permeable, stretch-activated nonselective cation (SA Cat) channels mediate cellular responses to mechanical stimuli. However, genes encoding such channels have not been identified in eukaryotes. The yeast MID1 gene product (Mid1) is required for calcium influx in the yeast Saccharomyces cerevisiae. Functional expression of Mid1 in Chinese hamster ovary cells conferred sensitivity to mechanical stress that resulted in increases in both calcium conductance and the concentration of cytosolic free calcium. These increases were dependent on the presence of extracellular calcium and were reduced by gadolinium, a blocker of SA Cat channels. Single-channel analyses with cell-attached patches revealed that Mid1 acts as a calcium-permeable, cation-selective stretch-activated channel with a conductance of 32 picosiemens at 150 millimolar cesium chloride in the pipette. Thus, Mid1 appears to be a eukaryotic, SA Cat channel.

1 Laboratory of Cell Biology, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma 371-8510, Japan.
2 Supermolecular Science Division, Electrotechnical Laboratory, Tsukuba, Ibaraki 305-8568, Japan.
3 Department of Physiology, Nagoya University School of Medicine, Nagoya, Aichi 466-8550, Japan.
4 Department of Biology, Tokyo Gakugei University, Koganei, Tokyo 184-8501, Japan.
*   To whom correspondence should be addressed. E-mail: iida{at}u-gakugei.ac.jp

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A role for calcium-calmodulin in regulating nitric oxide production during skeletal muscle satellite cell activation.
R. Tatsumi, A. L. Wuollet, K. Tabata, S. Nishimura, S. Tabata, W. Mizunoya, Y. Ikeuchi, and R. E. Allen (2009)
Am J Physiol Cell Physiol 296, C922-C929
   Abstract »    Full Text »    PDF »
Ion-channel blocker sensitivity of voltage-gated calcium-channel homologue Cch1 in Saccharomyces cerevisiae.
J. Teng, R. Goto, K. Iida, I. Kojima, and H. Iida (2008)
Microbiology 154, 3775-3781
   Abstract »    Full Text »    PDF »
Ion Channels in Microbes.
B. Martinac, Y. Saimi, and C. Kung (2008)
Physiol Rev 88, 1449-1490
   Abstract »    Full Text »    PDF »
A genome-wide survey suggests an osmoprotective role for vacuolar Ca2+ release in cell wall-compromised yeast.
S. Loukin, X. Zhou, C. Kung, and Y. Saimi (2008)
FASEB J 22, 2405-2415
   Abstract »    Full Text »    PDF »
Phenotype of a Mechanosensitive Channel Mutant, mid-1, in a Filamentous Fungus, Neurospora crassa.
R. R. Lew, Z. Abbas, M. I. Anderca, and S. J. Free (2008)
Eukaryot. Cell 7, 647-655
   Abstract »    Full Text »    PDF »
The L-Type Calcium Ion Channel Cch1 Affects Ascospore Discharge and Mycelial Growth in the Filamentous Fungus Gibberella zeae (Anamorph Fusarium graminearum).
H. E. Hallen and F. Trail (2008)
Eukaryot. Cell 7, 415-424
   Abstract »    Full Text »    PDF »
Essential, Completely Conserved Glycine Residue in the Domain III S2 S3 Linker of Voltage-gated Calcium Channel {alpha}1 Subunits in Yeast and Mammals.
K. Iida, J. Teng, T. Tada, A. Saka, M. Tamai, H. Izumi-Nakaseko, S. Adachi-Akahane, and H. Iida (2007)
J. Biol. Chem. 282, 25659-25667
   Abstract »    Full Text »    PDF »
Arabidopsis plasma membrane protein crucial for Ca2+ influx and touch sensing in roots.
Y. Nakagawa, T. Katagiri, K. Shinozaki, Z. Qi, H. Tatsumi, T. Furuichi, A. Kishigami, M. Sokabe, I. Kojima, S. Sato, et al. (2007)
PNAS 104, 3639-3644
   Abstract »    Full Text »    PDF »
Real-Time Monitoring of Calcineurin Activity in Living Cells: Evidence for Two Distinct Ca2+-dependent Pathways in Fission Yeast.
L. Deng, R. Sugiura, M. Takeuchi, M. Suzuki, H. Ebina, T. Takami, A. Koike, S. Iba, and T. Kuno (2006)
Mol. Biol. Cell 17, 4790-4800
   Abstract »    Full Text »    PDF »
Cch1 Mediates Calcium Entry in Cryptococcus neoformans and Is Essential in Low-Calcium Environments.
M. Liu, P. Du, G. Heinrich, G. M. Cox, and A. Gelli (2006)
Eukaryot. Cell 5, 1788-1796
   Abstract »    Full Text »    PDF »
Spatial Localization of m-Calpain to the Plasma Membrane by Phosphoinositide Biphosphate Binding during Epidermal Growth Factor Receptor-Mediated Activation..
H. Shao, J. Chou, C. J. Baty, N. A. Burke, S. C. Watkins, D. B. Stolz, and A. Wells (2006)
Mol. Cell. Biol. 26, 5481-5496
   Abstract »    Full Text »    PDF »
Polarized Morphogenesis Regulator Spa2 Is Required for the Function of Putative Stretch-Activated Ca2+-Permeable Channel Component Mid1 in Saccharomyces cerevisiae.
S. Noma, K. Iida, and H. Iida (2005)
Eukaryot. Cell 4, 1353-1363
   Abstract »    Full Text »    PDF »
Cell Wall Integrity Signaling in Saccharomyces cerevisiae.
D. E. Levin (2005)
Microbiol. Mol. Biol. Rev. 69, 262-291
   Abstract »    Full Text »    PDF »
Effects of Cyclic Tensile Forces on the Expression of Vascular Endothelial Growth Factor (VEGF) and Macrophage-colony-stimulating Factor (M-CSF) in Murine Osteoblastic MC3T3-E1 Cells.
M. Motokawa, M. Kaku, Y. Tohma, T. Kawata, T. Fujita, S. Kohno, K. Tsutsui, J. Ohtani, K. Tenjo, M. Shigekawa, et al. (2005)
Journal of Dental Research 84, 422-427
   Abstract »    Full Text »    PDF »
Possible Involvement of Mechanosensitive Ca2+ Channels of Plasma Membrane in Mechanoperception in Chara.
T. Kaneko, C. Saito, T. Shimmen, and M. Kikuyama (2005)
Plant Cell Physiol. 46, 130-135
   Abstract »    Full Text »    PDF »
Methylglyoxal, a Metabolite Derived from Glycolysis, Functions as a Signal Initiator of the High Osmolarity Glycerol-Mitogen-activated Protein Kinase Cascade and Calcineurin/Crz1-mediated Pathway in Saccharomyces cerevisiae.
K. Maeta, S. Izawa, and Y. Inoue (2005)
J. Biol. Chem. 280, 253-260
   Abstract »    Full Text »    PDF »
Differential Antifungal and Calcium Channel-Blocking Activity among Structurally Related Plant Defensins.
R. G. Spelbrink, N. Dilmac, A. Allen, T. J. Smith, D. M. Shah, and G. H. Hockerman (2004)
Plant Physiology 135, 2055-2067
   Abstract »    Full Text »    PDF »
TRPV2 Is a Component of Osmotically Sensitive Cation Channels in Murine Aortic Myocytes.
K. Muraki, Y. Iwata, Y. Katanosaka, T. Ito, S. Ohya, M. Shigekawa, and Y. Imaizumi (2003)
Circ. Res. 93, 829-838
   Abstract »    Full Text »    PDF »
Ca2+-Dependent Cessation of Cytoplasmic Streaming Induced by Hypertonic Treatment in Vallisneria Mesophyll Cells: Possible Role of Cell Wall-Plasma Membrane Adhesion.
T. Hayashi and S. Takagi (2003)
Plant Cell Physiol. 44, 1027-1036
   Abstract »    Full Text »    PDF »
Fig1p Facilitates Ca2+ Influx and Cell Fusion during Mating of Saccharomyces cerevisiae.
E. M. Muller, N. A. Mackin, S. E. Erdman, and K. W. Cunningham (2003)
J. Biol. Chem. 278, 38461-38469
   Abstract »    Full Text »    PDF »
The transient receptor potential channel on the yeast vacuole is mechanosensitive.
X.-L. Zhou, A. F. Batiza, S. H. Loukin, C. P. Palmer, C. Kung, and Y. Saimi (2003)
PNAS 100, 7105-7110
   Abstract »    Full Text »    PDF »
Molecular Dissection of the Hydrophobic Segments H3 and H4 of the Yeast Ca2+ Channel Component Mid1.
T. Tada, M. Ohmori, and H. Iida (2003)
J. Biol. Chem. 278, 9647-9654
   Abstract »    Full Text »    PDF »
Human Salivary Histatin 5 Causes Disordered Volume Regulation and Cell Cycle Arrest in Candida albicans.
D. Baev, X. S. Li, J. Dong, P. Keng, and M. Edgerton (2002)
Infect. Immun. 70, 4777-4784
   Abstract »    Full Text »    PDF »
An Osmotically Induced Cytosolic Ca2+ Transient Activates Calcineurin Signaling to Mediate Ion Homeostasis and Salt Tolerance of Saccharomyces cerevisiae.
T. K. Matsumoto, A. J. Ellsmore, S. G. Cessna, P. S. Low, J. M. Pardo, R. A. Bressan, and P. M. Hasegawa (2002)
J. Biol. Chem. 277, 33075-33080
   Abstract »    Full Text »    PDF »
Evidence for a role of platelet endothelial cell adhesion molecule-1 in endothelial cell mechanosignal transduction: is it a mechanoresponsive molecule?.
M. Osawa, M. Masuda, K.-i. Kusano, and K. Fujiwara (2002)
J. Cell Biol. 158, 773-785
   Abstract »    Full Text »    PDF »
A mechanogated nonselective cation channel in proximal tubule that is ATP sensitive.
C. G. Hurwitz, V. Y. Hu, and A. S. Segal (2002)
Am J Physiol Renal Physiol 283, F93-F104
   Abstract »    Full Text »    PDF »
Osmotic Stress Signaling and Osmoadaptation in Yeasts.
S. Hohmann (2002)
Microbiol. Mol. Biol. Rev. 66, 300-372
   Abstract »    Full Text »    PDF »
The Virally Encoded Fungal Toxin KP4 Specifically Blocks L-Type Voltage-Gated Calcium Channels.
M. J. Gage, S. G. Rane, G. H. Hockerman, and T. J. Smith (2002)
Mol. Pharmacol. 61, 936-944
   Abstract »    Full Text »    PDF »
Essential Hydrophilic Carboxyl-terminal Regions Including Cysteine Residues of the Yeast Stretch-activated Calcium-permeable Channel Mid1.
T. Maruoka, Y. Nagasoe, S. Inoue, Y. Mori, J. Goto, M. Ikeda, and H. Iida (2002)
J. Biol. Chem. 277, 11645-11652
   Abstract »    Full Text »    PDF »
Differential Regulation of Two Ca2+ Influx Systems by Pheromone Signaling in Saccharomyces cerevisiae.
E. M. Muller, E. G. Locke, and K. W. Cunningham (2001)
Genetics 159, 1527-1538
   Abstract »    Full Text »    PDF »
Ion Channels and Their Functional Role in Vascular Endothelium.
B. Nilius and G. Droogmans (2001)
Physiol Rev 81, 1415-1459
   Abstract »    Full Text »    PDF »
A Putative Two Pore Channel AtTPC1 Mediates Ca2+ Flux in Arabidopsis Leaf Cells.
T. Furuichi, K. W. Cunningham, and S. Muto (2001)
Plant Cell Physiol. 42, 900-905
   Abstract »    Full Text »    PDF »
A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca2+-permeable channel in the yeast vacuolar membrane.
C. P. Palmer, X.-L. Zhou, J. Lin, S. H. Loukin, C. Kung, and Y. Saimi (2001)
PNAS
   Abstract »    Full Text »    PDF »
Molecular Basis of Mechanotransduction in Living Cells.
O. P. Hamill and B. Martinac (2001)
Physiol Rev 81, 685-740
   Abstract »    Full Text »    PDF »
Tryptophan Permease Gene TAT2 Confers High-Pressure Growth in Saccharomyces cerevisiae.
F. Abe and K. Horikoshi (2000)
Mol. Cell. Biol. 20, 8093-8102
   Abstract »    Full Text »
A Homolog of Voltage-Gated Ca2+ Channels Stimulated by Depletion of Secretory Ca2+ in Yeast.
E. G. Locke, M. Bonilla, L. Liang, Y. Takita, and K. W. Cunningham (2000)
Mol. Cell. Biol. 20, 6686-6694
   Abstract »    Full Text »
Whole-cell and single channel monovalent cation currents through the novel rabbit epithelial Ca2+ channel ECaC.
B. Nilius, R. Vennekens, J. Prenen, J. G J Hoenderop, R. J M Bindels, and G. Droogmans (2000)
J. Physiol. 527, 239-248
   Abstract »    Full Text »    PDF »
A Drosophila Mechanosensory Transduction Channel.
R. G. Walker, A. T. Willingham, and C. S. Zuker (2000)
Science 287, 2229-2234
   Abstract »    Full Text »
Permeation and Gating Properties of the Novel Epithelial Ca2+ Channel.
R. Vennekens, J. G. J. Hoenderop, J. Prenen, M. Stuiver, P. H. G. M. Willems, G. Droogmans, B. Nilius, and R. J. M. Bindels (2000)
J. Biol. Chem. 275, 3963-3969
   Abstract »    Full Text »    PDF »
The Single Pore Residue Asp542 Determines Ca2+ Permeation and Mg2+ Block of the Epithelial Ca2+ Channel.
B. Nilius, R. Vennekens, J. Prenen, J. G. J. Hoenderop, G. Droogmans, and R. J. M. Bindels (2001)
J. Biol. Chem. 276, 1020-1025
   Abstract »    Full Text »    PDF »
Identification of a Novel Chloride Channel Expressed in the Endoplasmic Reticulum, Golgi Apparatus, and Nucleus.
M. Nagasawa, M. Kanzaki, Y. Iino, Y. Morishita, and I. Kojima (2001)
J. Biol. Chem. 276, 20413-20418
   Abstract »    Full Text »    PDF »
A TRP homolog in Saccharomyces cerevisiae forms an intracellular Ca2+-permeable channel in the yeast vacuolar membrane.
C. P. Palmer, X.-L. Zhou, J. Lin, S. H. Loukin, C. Kung, and Y. Saimi (2001)
PNAS 98, 7801-7805
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)