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Science 30 July 1999:
Vol. 285. no. 5428, pp. 754 - 756
DOI: 10.1126/science.285.5428.754
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Reports

Embryonic Stem Cell-Derived Glial Precursors: A Source of Myelinating Transplants

Oliver Brüstle, 1* Kimberly N. Jones, 12 Randall D. Learish, 3dagger Khalad Karram, 1 Khalid Choudhary, 12 Otmar D. Wiestler, 1 Ian D. Duncan, 3 Ronald D. G. McKay 2

Self-renewing, totipotent embryonic stem (ES) cells may provide a virtually unlimited donor source for transplantation. A protocol that permits the in vitro generation of precursors for oligodendrocytes and astrocytes from ES cells was devised. Transplantation in a rat model of a human myelin disease shows that these ES cell-derived precursors interact with host neurons and efficiently myelinate axons in brain and spinal cord. Thus, ES cells can serve as a valuable source of cell type-specific somatic precursors for neural transplantation.

1 Department of Neuropathology, University of Bonn Medical Center, Sigmund-Freud-Straße 25, 53105 Bonn, Germany.
2 Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892-4092, USA.
3 Department of Medical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA.
*   To whom correspondence should be addressed. E-mail: brustle{at}uni-bonn.de

dagger    Present address: Promega Corp., 2800 Woods Hollow Road, Madison, WI 53711, USA.

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PNAS 98, 15014-15019
   Abstract »    Full Text »    PDF »
Stem Cells: New Opportunities to Treat Eye Diseases.
I. Ahmad (2001)
Invest. Ophthalmol. Vis. Sci. 42, 2743-2748
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Towards the reconstruction of central nervous system white matter using neural precursor cells.
M. Mitome, H. P. Low, A. van den Pol, J. J. Nunnari, M. K. Wolf, S. Billings-Gagliardi, and W. J. Schwartz (2001)
Brain 124, 2147-2161
   Abstract »    Full Text »    PDF »
Nestin-Specific Green Fluorescent Protein Expression in Embryonic Stem Cell-Derived Neural Precursor Cells Used for Transplantation.
C. Andressen, E. Stocker, F.-J. Klinz, N. Lenka, J. Hescheler, B. Fleischmann, S. Arnhold, and K. Addicks (2001)
Stem Cells 19, 419-424
   Abstract »    Full Text »    PDF »
Toxic Leukoencephalopathy.
C. M. Filley and B.K. Kleinschmidt-DeMasters (2001)
N. Engl. J. Med. 345, 425-432
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Stem Cells: A Journey into a New Frontier.
B. E. PETERSEN and N. TERADA (2001)
J. Am. Soc. Nephrol. 12, 1773-1780
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Differentiation of Embryonic Stem Cell Lines Generated from Adult Somatic Cells by Nuclear Transfer.
T. Wakayama, V. Tabar, I. Rodriguez, A. C. F. Perry, L. Studer, and P. Mombaerts (2001)
Science 292, 740-743
   Abstract »    Full Text »
The Spinal Cord Research Foundation: Two Decades of Progress.
M. S. Kelley (2001)
Neuroscientist 7, 104-112
   Abstract »    PDF »
Gene and Stem Cell Therapies.
E. H. Kaji and J. M. Leiden (2001)
JAMA 285, 545-550
   Abstract »    Full Text »    PDF »


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