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Science 30 July 1999:
Vol. 285. no. 5428, pp. 739 - 743
DOI: 10.1126/science.285.5428.739
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Reports

Genetic Mechanisms of Age Regulation of Human Blood Coagulation Factor IX

Sumiko Kurachi, Yoshihiro Deyashiki, Junko Takeshita, Kotoku Kurachi *

Blood coagulation capacity increases with age in healthy individuals. Through extensive longitudinal analyses of human factor IX gene expression in transgenic mice, two essential age-regulatory elements, AE5' and AE3', have been identified. These elements are required and together are sufficient for normal age regulation of factor IX expression. AE5', a PEA-3 related element present in the 5' upstream region of the gene encoding factor IX, is responsible for age-stable expression of the gene. AE3', in the middle of the 3' untranslated region, is responsible for age-associated elevation in messenger RNA levels. In a concerted manner, AE5' and AE3' recapitulate natural patterns of the advancing age-associated increase in factor IX gene expression.

Department of Human Genetics, University of Michigan Medical School, Ann Arbor, MI 48109-0618, USA. E-mail: kkurachi{at}umich.edu
*   To whom correspondence should be addressed.

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