Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 9 July 1999:
Vol. 285. no. 5425, pp. 263 - 265
DOI: 10.1126/science.285.5425.263
Prev | Table of Contents

Reports

hRAD30 Mutations in the Variant Form of Xeroderma Pigmentosum

Robert E. Johnson, Christine M. Kondratick, Satya Prakash, Louise Prakash *

Xeroderma pigmentosum (XP) is an autosomal recessive disease characterized by a high incidence of skin cancers. Yeast RAD30 encodes a DNA polymerase involved in the error-free bypass of ultraviolet (UV) damage. Here it is shown that XP variant (XP-V) cell lines harbor nonsense or frameshift mutations in hRAD30, the human counterpart of yeast RAD30. Of the eight mutations identified, seven would result in a severely truncated hRad30 protein. These results indicate that defects in hRAD30 cause XP-V, and they suggest that error-free replication of UV lesions by hRad30 plays an important role in minimizing the incidence of sunlight-induced skin cancers.

Sealy Center for Molecular Science, University of Texas Medical Branch at Galveston, 6.104 Medical Research Building, 11th and Mechanic Streets, Galveston, TX 77555-1061, USA.
*   To whom correspondence should be addressed. E-mail: lprakash{at}scms.utmb.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A real-time fluorescence method for enzymatic characterization of specialized human DNA polymerases.
D. Dorjsuren, D. M. Wilson III, W. A. Beard, J. P. McDonald, C. P. Austin, R. Woodgate, S. H. Wilson, and A. Simeonov (2009)
Nucleic Acids Res. 37, e128
   Abstract »    Full Text »    PDF »
A model for DNA polymerase switching involving a single cleft and the rim of the sliding clamp.
J. M. H. Heltzel, R. W. Maul, S. K. Scouten Ponticelli, and M. D. Sutton (2009)
PNAS 106, 12664-12669
   Abstract »    Full Text »    PDF »
DNA polymerase {zeta} cooperates with polymerases {kappa} and {iota} in translesion DNA synthesis across pyrimidine photodimers in cells from XPV patients.
O. Ziv, N. Geacintov, S. Nakajima, A. Yasui, and Z. Livneh (2009)
PNAS 106, 11552-11557
   Abstract »    Full Text »    PDF »
Yeast Rev1 protein promotes complex formation of DNA polymerase {zeta} with Pol32 subunit of DNA polymerase {delta}.
N. Acharya, R. E. Johnson, V. Pages, L. Prakash, and S. Prakash (2009)
PNAS 106, 9631-9636
   Abstract »    Full Text »    PDF »
Human DNA Polymerase {eta} Is Required for Common Fragile Site Stability during Unperturbed DNA Replication.
L. Rey, J. M. Sidorova, N. Puget, F. Boudsocq, D. S. F. Biard, R. J. Monnat Jr., C. Cazaux, and J.-S. Hoffmann (2009)
Mol. Cell. Biol. 29, 3344-3354
   Abstract »    Full Text »    PDF »
A Backup Role of DNA Polymerase {kappa} in Ig Gene Hypermutation Only Takes Place in the Complete Absence of DNA Polymerase {eta}.
A. Faili, A. Stary, F. Delbos, S. Weller, S. Aoufouchi, A. Sarasin, J.-C. Weill, and C.-A. Reynaud (2009)
J. Immunol. 182, 6353-6359
   Abstract »    Full Text »    PDF »
Structural Basis for Novel Interactions between Human Translesion Synthesis Polymerases and Proliferating Cell Nuclear Antigen.
A. Hishiki, H. Hashimoto, T. Hanafusa, K. Kamei, E. Ohashi, T. Shimizu, H. Ohmori, and M. Sato (2009)
J. Biol. Chem. 284, 10552-10560
   Abstract »    Full Text »    PDF »
Somatic hypermutation of immunoglobulin genes: lessons from proliferating cell nuclear antigenK164R mutant mice.
P. Langerak, P. H.L Krijger, M. R Heideman, P. C.M van den Berk, and H. Jacobs (2009)
Phil Trans R Soc B 364, 621-629
   Abstract »    Full Text »    PDF »
Eukaryotic Translesion Polymerases and Their Roles and Regulation in DNA Damage Tolerance.
L. S. Waters, B. K. Minesinger, M. E. Wiltrout, S. D'Souza, R. V. Woodruff, and G. C. Walker (2009)
Microbiol. Mol. Biol. Rev. 73, 134-154
   Abstract »    Full Text »    PDF »
Identification of a novel REV1-interacting motif necessary for DNA polymerase kappa function..
E. Ohashi, T. Hanafusa, K. Kamei, I. Song, J. Tomida, H. Hashimoto, C. Vaziri, and H. Ohmori (2009)
Genes Cells 14, 101-111
   Abstract »    Full Text »    PDF »
Effect of Proliferating Cell Nuclear Antigen Ubiquitination and Chromatin Structure on the Dynamic Properties of the Y-family DNA Polymerases.
S. Sabbioneda, A. M. Gourdin, C. M. Green, A. Zotter, G. Giglia-Mari, A. Houtsmuller, W. Vermeulen, and A. R. Lehmann (2008)
Mol. Biol. Cell 19, 5193-5202
   Abstract »    Full Text »    PDF »
Roles of PCNA-binding and ubiquitin-binding domains in human DNA polymerase {eta} in translesion DNA synthesis.
N. Acharya, J.-H. Yoon, H. Gali, I. Unk, L. Haracska, R. E. Johnson, J. Hurwitz, L. Prakash, and S. Prakash (2008)
PNAS 105, 17724-17729
   Abstract »    Full Text »    PDF »
Requirement of Rad5 for DNA Polymerase {zeta}-Dependent Translesion Synthesis in Saccharomyces cerevisiae.
V. Pages, A. Bresson, N. Acharya, S. Prakash, R. P. Fuchs, and L. Prakash (2008)
Genetics 180, 73-82
   Abstract »    Full Text »    PDF »
Recognition of forked and single-stranded DNA structures by human RAD18 complexed with RAD6B protein triggers its recruitment to stalled replication forks..
Y. Tsuji, K. Watanabe, K. Araki, M. Shinohara, Y. Yamagata, T. Tsurimoto, F. Hanaoka, K.-i. Yamamura, M. Yamaizumi, and S. Tateishi (2008)
Genes Cells 13, 343-354
   Abstract »    Full Text »    PDF »
Substitution of a residue contacting the triphosphate moiety of the incoming nucleotide increases the fidelity of yeast DNA polymerase {zeta}.
C. A. Howell, C. M. Kondratick, and M. T. Washington (2008)
Nucleic Acids Res. 36, 1731-1740
   Abstract »    Full Text »    PDF »
Xeroderma pigmentosum genes: functions inside and outside DNA repair.
K. Sugasawa (2008)
Carcinogenesis 29, 455-465
   Abstract »    Full Text »    PDF »
Complex Formation of Yeast Rev1 with DNA Polymerase {eta}.
N. Acharya, L. Haracska, S. Prakash, and L. Prakash (2007)
Mol. Cell. Biol. 27, 8401-8408
   Abstract »    Full Text »    PDF »
Bypass of DNA Lesions Generated During Anticancer Treatment with Cisplatin by DNA Polymerase {eta}.
A. Alt, K. Lammens, C. Chiocchini, A. Lammens, J. C. Pieck, D. Kuch, K.-P. Hopfner, and T. Carell (2007)
Science 318, 967-970
   Abstract »    Full Text »    PDF »
Requirement of RAD52 Group Genes for Postreplication Repair of UV-Damaged DNA in Saccharomyces cerevisiae.
V. Gangavarapu, S. Prakash, and L. Prakash (2007)
Mol. Cell. Biol. 27, 7758-7764
   Abstract »    Full Text »    PDF »
A Role for Yeast and Human Translesion Synthesis DNA Polymerases in Promoting Replication through 3-Methyl Adenine.
R. E. Johnson, S.-L. Yu, S. Prakash, and L. Prakash (2007)
Mol. Cell. Biol. 27, 7198-7205
   Abstract »    Full Text »    PDF »
What a difference a decade makes: Insights into translesion DNA synthesis.
W. Yang and R. Woodgate (2007)
PNAS 104, 15591-15598
   Abstract »    Full Text »    PDF »
Biochemical reconstitution of abasic DNA lesion replication in Xenopus extracts.
S. Liao, Y. Matsumoto, and H. Yan (2007)
Nucleic Acids Res.
   Abstract »    Full Text »    PDF »
Werner syndrome protein interacts functionally with translesion DNA polymerases.
A. S. Kamath-Loeb, L. Lan, S. Nakajima, A. Yasui, and L. A. Loeb (2007)
PNAS 104, 10394-10399
   Abstract »    Full Text »    PDF »
The Human Tim/Tipin Complex Coordinates an Intra-S Checkpoint Response to UV That Slows Replication Fork Displacement.
K. Unsal-Kacmaz, P. D. Chastain, P.-P. Qu, P. Minoo, M. Cordeiro-Stone, A. Sancar, and W. K. Kaufmann (2007)
Mol. Cell. Biol. 27, 3131-3142
   Abstract »    Full Text »    PDF »
Evidence that in Xeroderma Pigmentosum Variant Cells, which Lack DNA Polymerase {eta}, DNA Polymerase {iota} Causes the Very High Frequency and Unique Spectrum of UV-Induced Mutations.
Y. Wang, R. Woodgate, T. P. McManus, S. Mead, J. J. McCormick, and V. M. Maher (2007)
Cancer Res. 67, 3018-3026
   Abstract »    Full Text »    PDF »
Telomere Dysfunction Drives Increased Mutation by Error-Prone Polymerases Rev1 and {zeta} in Saccharomyces cerevisiae.
D. H. Meyer and A. M. Bailis (2007)
Genetics 175, 1533-1537
   Abstract »    Full Text »    PDF »
Complex Formation with Rev1 Enhances the Proficiency of Saccharomyces cerevisiae DNA Polymerase {zeta} for Mismatch Extension and for Extension Opposite from DNA Lesions.
N. Acharya, R. E. Johnson, S. Prakash, and L. Prakash (2006)
Mol. Cell. Biol. 26, 9555-9563
   Abstract »    Full Text »    PDF »
Role of Pseudomonas aeruginosa dinB-Encoded DNA Polymerase IV in Mutagenesis.
L. H. Sanders, A. Rockel, H. Lu, D. J. Wozniak, and M. D. Sutton (2006)
J. Bacteriol. 188, 8573-8585
   Abstract »    Full Text »    PDF »
Human SHPRH suppresses genomic instability through proliferating cell nuclear antigen polyubiquitination.
A. Motegi, R. Sood, H. Moinova, S. D. Markowitz, P. P. Liu, and K. Myung (2006)
J. Cell Biol. 175, 703-708
   Abstract »    Full Text »    PDF »
Activation of the Fanconi Anemia/BRCA Pathway and Recombination Repair in the Cellular Response to Solar Ultraviolet Light.
J. Dunn, M. Potter, A. Rees, and T. M. Runger (2006)
Cancer Res. 66, 11140-11147
   Abstract »    Full Text »    PDF »
Human SHPRH is a ubiquitin ligase for Mms2-Ubc13-dependent polyubiquitylation of proliferating cell nuclear antigen.
I. Unk, I. Hajdu, K. Fatyol, B. Szakal, A. Blastyak, V. Bermudez, J. Hurwitz, L. Prakash, S. Prakash, and L. Haracska (2006)
PNAS 103, 18107-18112
   Abstract »    Full Text »    PDF »
Participation of mouse DNA polymerase {iota} in strand-biased mutagenic bypass of UV photoproducts and suppression of skin cancer.
C. A. Dumstorf, A. B. Clark, Q. Lin, G. E. Kissling, T. Yuan, R. Kucherlapati, W. G. McGregor, and T. A. Kunkel (2006)
PNAS 103, 18083-18088
   Abstract »    Full Text »    PDF »
Human DNA polymerase {kappa} forms nonproductive complexes with matched primer termini but not with mismatched primer termini.
K. D. Carlson, R. E. Johnson, L. Prakash, S. Prakash, and M. T. Washington (2006)
PNAS 103, 15776-15781
   Abstract »    Full Text »    PDF »
Mms2-Ubc13-Dependent and -Independent Roles of Rad5 Ubiquitin Ligase in Postreplication Repair and Translesion DNA Synthesis in Saccharomyces cerevisiae.
V. Gangavarapu, L. Haracska, I. Unk, R. E. Johnson, S. Prakash, and L. Prakash (2006)
Mol. Cell. Biol. 26, 7783-7790
   Abstract »    Full Text »    PDF »
A human DNA polymerase eta complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest..
M. S. Yuasa, C. Masutani, A. Hirano, M. A. Cohn, M. Yamaizumi, Y. Nakatani, and F. Hanaoka (2006)
Genes Cells 11, 731-744
   Abstract »    Full Text »    PDF »
Ubiquitylation of yeast proliferating cell nuclear antigen and its implications for translesion DNA synthesis.
L. Haracska, I. Unk, L. Prakash, and S. Prakash (2006)
PNAS 103, 6477-6482
   Abstract »    Full Text »    PDF »
Frame shuffling: a novel method for in vitro protein evolution.
K. Kashiwagi, Y. Isogai, K.-I. Nishiguchi, and K. Shiba (2006)
Protein Eng. Des. Sel. 19, 135-140
   Abstract »    Full Text »    PDF »
Analyses of ultraviolet-induced focus formation of hREV1 protein.
Y. Murakumo, S. Mizutani, M. Yamaguchi, M. Ichihara, and M. Takahashi (2006)
Genes Cells 11, 193-205
   Abstract »    Full Text »    PDF »
DNA Polymerase {eta}, the Product of the Xeroderma Pigmentosum Variant Gene and a Target of p53, Modulates the DNA Damage Checkpoint and p53 Activation.
G. Liu and X. Chen (2006)
Mol. Cell. Biol. 26, 1398-1413
   Abstract »    Full Text »    PDF »
Regulation of Gross Chromosomal Rearrangements by Ubiquitin and SUMO Ligases in Saccharomyces cerevisiae.
A. Motegi, K. Kuntz, A. Majeed, S. Smith, and K. Myung (2006)
Mol. Cell. Biol. 26, 1424-1433
   Abstract »    Full Text »    PDF »
Increased Susceptibility to UV-Induced Skin Carcinogenesis in Polymerase {eta}-deficient Mice.
Q. Lin, A. B. Clark, S. D. McCulloch, T. Yuan, R. T. Bronson, T. A. Kunkel, and R. Kucherlapati (2006)
Cancer Res. 66, 87-94
   Abstract »    Full Text »    PDF »
Error-prone Translesion Synthesis by Human DNA Polymerase {eta} on DNA-containing Deoxyadenosine Adducts of 7,8-Dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene.
D. Chiapperino, M. Cai, J. M. Sayer, H. Yagi, H. Kroth, C. Masutani, F. Hanaoka, D. M. Jerina, and A. M. Cheh (2005)
J. Biol. Chem. 280, 39684-39692
   Abstract »    Full Text »    PDF »
A Role for Polymerase {eta} in the Cellular Tolerance to Cisplatin-Induced Damage.
M. R. Albertella, C. M. Green, A. R. Lehmann, and M. J. O'Connor (2005)
Cancer Res. 65, 9799-9806
   Abstract »    Full Text »    PDF »
Human DNA Polymerase {iota} Promotes Replication through a Ring-Closed Minor-Groove Adduct That Adopts a syn Conformation in DNA.
W. T. Wolfle, R. E. Johnson, I. G. Minko, R. S. Lloyd, S. Prakash, and L. Prakash (2005)
Mol. Cell. Biol. 25, 8748-8754
   Abstract »    Full Text »    PDF »
Distinct mechanisms of cis-syn thymine dimer bypass by Dpo4 and DNA polymerase {eta}.
R. E. Johnson, L. Prakash, and S. Prakash (2005)
PNAS 102, 12359-12364
   Abstract »    Full Text »    PDF »
Error-prone and inefficient replication across 8-hydroxyguanine (8-oxoguanine) in human and mouse ras gene fragments by DNA polymerase {kappa}.
P. Jaloszynski, E. Ohashi, H. Ohmori, and S. Nishimura (2005)
Genes Cells 10, 543-550
   Abstract »    Full Text »    PDF »
Mechanism of Efficient and Accurate Nucleotide Incorporation Opposite 7,8-Dihydro-8-Oxoguanine by Saccharomyces cerevisiae DNA Polymerase {eta}.
K. D. Carlson and M. T. Washington (2005)
Mol. Cell. Biol. 25, 2169-2176
   Abstract »    Full Text »    PDF »
Localisation of human Y-family DNA polymerase {kappa}: relationship to PCNA foci.
T. Ogi, P. Kannouche, and A. R. Lehmann (2005)
J. Cell Sci. 118, 129-136
   Abstract »    Full Text »    PDF »
Quantitative Analysis of Translesion DNA Synthesis across a Benzo[a]pyrene-Guanine Adduct in Mammalian Cells: THE ROLE OF DNA POLYMERASE {kappa}.
S. Avkin, M. Goldsmith, S. Velasco-Miguel, N. Geacintov, E. C. Friedberg, and Z. Livneh (2004)
J. Biol. Chem. 279, 53298-53305
   Abstract »    Full Text »    PDF »
DNA binding properties of human DNA polymerase {eta}: implications for fidelity and polymerase switching of translesion synthesis.
R. Kusumoto, C. Masutani, S. Shimmyo, S. Iwai, and F. Hanaoka (2004)
Genes Cells 9, 1139-1150
   Abstract »    Full Text »    PDF »
Proliferating Cell Nuclear Antigen-dependent Coordination of the Biological Functions of Human DNA Polymerase {iota}.
A. E. Vidal, P. Kannouche, V. N. Podust, W. Yang, A. R. Lehmann, and R. Woodgate (2004)
J. Biol. Chem. 279, 48360-48368
   Abstract »    Full Text »    PDF »
UV Light-induced DNA Damage and Tolerance for the Survival of Nucleotide Excision Repair-deficient Human Cells.
S. Nakajima, L. Lan, S.-i. Kanno, M. Takao, K. Yamamoto, A. P. M. Eker, and A. Yasui (2004)
J. Biol. Chem. 279, 46674-46677
   Abstract »    Full Text »    PDF »
The Role of DNA Polymerase {eta} in Translesion Synthesis Past Platinum-DNA Adducts in Human Fibroblasts.
E. Bassett, N. M. King, M. F. Bryant, S. Hector, L. Pendyala, S. G. Chaney, and M. Cordeiro-Stone (2004)
Cancer Res. 64, 6469-6475
   Abstract »    Full Text »    PDF »
Enzymatic switching for efficient and accurate translesion DNA replication.
S. D. McCulloch, R. J. Kokoska, O. Chilkova, C. M. Welch, E. Johansson, P. M. J. Burgers, and T. A. Kunkel (2004)
Nucleic Acids Res. 32, 4665-4675
   Abstract »    Full Text »    PDF »
Efficient and Error-Free Replication past a Minor-Groove N2-Guanine Adduct by the Sequential Action of Yeast Rev1 and DNA Polymerase {zeta}.
M. T. Washington, I. G. Minko, R. E. Johnson, L. Haracska, T. M. Harris, R. S. Lloyd, S. Prakash, and L. Prakash (2004)
Mol. Cell. Biol. 24, 6900-6906
   Abstract »    Full Text »    PDF »
Altered DNA Polymerase {iota} Expression in Breast Cancer Cells Leads to a Reduction in DNA Replication Fidelity and a Higher Rate of Mutagenesis.
J. Yang, Z. Chen, Y. Liu, R. J. Hickey, and L. H. Malkas (2004)
Cancer Res. 64, 5597-5607
   Abstract »    Full Text »    PDF »
CDC7/DBF4 Functions in the Translesion Synthesis Branch of the RAD6 Epistasis Group in Saccharomyces cerevisiae.
L. Pessoa-Brandao and R. A. Sclafani (2004)
Genetics 167, 1597-1610
   Abstract »    Full Text »    PDF »
Investigating the Role of the Little Finger Domain of Y-family DNA Polymerases in Low Fidelity Synthesis and Translesion Replication.
F. Boudsocq, R. J. Kokoska, B. S. Plosky, A. Vaisman, H. Ling, T. A. Kunkel, W. Yang, and R. Woodgate (2004)
J. Biol. Chem. 279, 32932-32940
   Abstract »    Full Text »    PDF »
Efficient and Error-Free Replication Past a Minor-Groove DNA Adduct by the Sequential Action of Human DNA Polymerases {iota} and {kappa}.
M. T. Washington, I. G. Minko, R. E. Johnson, W. T. Wolfle, T. M. Harris, R. S. Lloyd, S. Prakash, and L. Prakash (2004)
Mol. Cell. Biol. 24, 5687-5693
   Abstract »    Full Text »    PDF »
The Ubiquitin-Conjugating DNA Repair Enzyme HR6A Is a Maternal Factor Essential for Early Embryonic Development in Mice.
H. P. Roest, W. M. Baarends, J. de Wit, J. W. van Klaveren, E. Wassenaar, J. W. Hoogerbrugge, W. A. van Cappellen, J. H. J. Hoeijmakers, and J. A. Grootegoed (2004)
Mol. Cell. Biol. 24, 5485-5495
   Abstract »    Full Text »    PDF »
Interaction of hREV1 with three human Y-family DNA polymerases.
E. Ohashi, Y. Murakumo, N. Kanjo, J.-i. Akagi, C. Masutani, F. Hanaoka, and H. Ohmori (2004)
Genes Cells 9, 523-531
   Abstract »    Full Text »    PDF »
Enhancement of the Mutagenicity of Benzo(a)pyrene Diol Epoxide by a Nonmutagenic Dose of Ultraviolet A Radiation.
A. Besaratinia and G. P. Pfeifer (2003)
Cancer Res. 63, 8708-8716
   Abstract »    Full Text »    PDF »
The Efficiency and Specificity of Apurinic/Apyrimidinic Site Bypass by Human DNA Polymerase {eta} and Sulfolobus solfataricus Dpo4.
R. J. Kokoska, S. D. McCulloch, and T. A. Kunkel (2003)
J. Biol. Chem. 278, 50537-50545
   Abstract »    Full Text »    PDF »
The Mechanism of Nucleotide Incorporation by Human DNA Polymerase {eta} Differs from That of the Yeast Enzyme.
M. T. Washington, R. E. Johnson, L. Prakash, and S. Prakash (2003)
Mol. Cell. Biol. 23, 8316-8322
   Abstract »    Full Text »    PDF »
A mechanism for the exclusion of low-fidelity human Y-family DNA polymerases from base excision repair.
L. Haracska, L. Prakash, and S. Prakash (2003)
Genes & Dev. 17, 2777-2785
   Abstract »    Full Text »    PDF »
8-Hydroxyguanine in a mutational hotspot of the c-Ha-ras gene causes misreplication, 'action-at-a-distance' mutagenesis and inhibition of replication.
P. Jaloszynski, C. Masutani, F. Hanaoka, A. B. Perez, and S. Nishimura (2003)
Nucleic Acids Res. 31, 6085-6095
   Abstract »    Full Text »    PDF »
Mechanism of nucleotide incorporation opposite a thymine-thymine dimer by yeast DNA polymerase {eta}.
M. T. Washington, L. Prakash, and S. Prakash (2003)
PNAS 100, 12093-12098
   Abstract »    Full Text »    PDF »
Localization of the Deoxyribose Phosphate Lyase Active Site in Human DNA Polymerase {iota} by Controlled Proteolysis.
R. Prasad, K. Bebenek, E. Hou, D. D. Shock, W. A. Beard, R. Woodgate, T. A. Kunkel, and S. H. Wilson (2003)
J. Biol. Chem. 278, 29649-29654
   Abstract »    Full Text »    PDF »
Requirement of Watson-Crick Hydrogen Bonding for DNA Synthesis by Yeast DNA Polymerase {eta}.
M. T. Washington, S. A. Helquist, E. T. Kool, L. Prakash, and S. Prakash (2003)
Mol. Cell. Biol. 23, 5107-5112
   Abstract »    Full Text »    PDF »
NRAS Hypermutability in Familial Melanoma With CDKN2A Mutations--Cause and Effect?.
K. H. Kraemer (2003)
J Natl Cancer Inst 95, 768-769
   Full Text »    PDF »
Role of DNA Polymerase {eta} in the UV Mutation Spectrum in Human Cells.
A. Stary, P. Kannouche, A. R. Lehmann, and A. Sarasin (2003)
J. Biol. Chem. 278, 18767-18775
   Abstract »    Full Text »    PDF »
Amino Acid Substitutions at Conserved Tyrosine 52 Alter Fidelity and Bypass Efficiency of Human DNA Polymerase {eta}.
E. Glick, J. S. Chau, K. L. Vigna, S. D. McCulloch, E. T. Adman, T. A. Kunkel, and L. A. Loeb (2003)
J. Biol. Chem. 278, 19341-19346
   Abstract »    Full Text »    PDF »
Yeast DNA polymerase eta makes functional contacts with the DNA minor groove only at the incoming nucleoside triphosphate.
M. T. Washington, W. T. Wolfle, T. E. Spratt, L. Prakash, and S. Prakash (2003)
PNAS 100, 5113-5118
   Abstract »    Full Text »    PDF »
Deoxynucleotide Triphosphate Binding Mode Conserved in Y Family DNA Polymerases.
R. E. Johnson, J. Trincao, A. K. Aggarwal, S. Prakash, and L. Prakash (2003)
Mol. Cell. Biol. 23, 3008-3012
   Abstract »    Full Text »    PDF »
Mammalian Translesion DNA Synthesis across an Acrolein-derived Deoxyguanosine Adduct. PARTICIPATION OF DNA POLYMERASE eta IN ERROR-PRONE SYNTHESIS IN HUMAN CELLS.
I.-Y. Yang, H. Miller, Z. Wang, E. G. Frank, H. Ohmori, F. Hanaoka, and M. Moriya (2003)
J. Biol. Chem. 278, 13989-13994
   Abstract »    Full Text »    PDF »
Yeast DNA Polymerase {zeta} Is an Efficient Extender of Primer Ends Opposite from 7,8-Dihydro-8-Oxoguanine and O6-Methylguanine.
L. Haracska, S. Prakash, and L. Prakash (2003)
Mol. Cell. Biol. 23, 1453-1459
   Abstract »    Full Text »    PDF »
Enhanced Genomic Instability and Defective Postreplication Repair in RAD18 Knockout Mouse Embryonic Stem Cells.
S. Tateishi, H. Niwa, J.-I. Miyazaki, S. Fujimoto, H. Inoue, and M. Yamaizumi (2003)
Mol. Cell. Biol. 23, 474-481
   Abstract »    Full Text »    PDF »
Nucleotide Excision Repair- and Polymerase {eta}-Mediated Error-Prone Removal of Mitomycin C Interstrand Cross-Links.
H. Zheng, X. Wang, A. J. Warren, R. J. Legerski, R. S. Nairn, J. W. Hamilton, and L. Li (2003)
Mol. Cell. Biol. 23, 754-761
   Abstract »    Full Text »    PDF »
Translesion Synthesis past Acrolein-derived DNA Adduct, gamma -Hydroxypropanodeoxyguanosine, by Yeast and Human DNA Polymerase eta.
I. G. Minko, M. T. Washington, M. Kanuri, L. Prakash, S. Prakash, and R. S. Lloyd (2003)
J. Biol. Chem. 278, 784-790
   Abstract »    Full Text »    PDF »
A Role for DNA Polymerase beta in Mutagenic UV Lesion Bypass.
L. Servant, C. Cazaux, A. Bieth, S. Iwai, F. Hanaoka, and J.-S. Hoffmann (2002)
J. Biol. Chem. 277, 50046-50053
   Abstract »    Full Text »    PDF »
Role of human DNA polymerase kappa as an extender in translesion synthesis.
L. Haracska, L. Prakash, and S. Prakash (2002)
PNAS 99, 16000-16005
   Abstract »    Full Text »    PDF »
Involvement of Vertebrate Polkappa in Rad18-independent Postreplication Repair of UV Damage.
T. Okada, E. Sonoda, Y. M. Yamashita, S. Koyoshi, S. Tateishi, M. Yamaizumi, M. Takata, O. Ogawa, and S. Takeda (2002)
J. Biol. Chem. 277, 48690-48695
   Abstract »    Full Text »    PDF »
Polkappa protects mammalian cells against the lethal and mutagenic effects of benzo[a]pyrene.
T. Ogi, Y. Shinkai, K. Tanaka, and H. Ohmori (2002)
PNAS 99, 15548-15553
   Abstract »    Full Text »    PDF »
The Role of Polymerase {eta} in Somatic Hypermutation Determined by Analysis of Mutations in a Patient with Xeroderma Pigmentosum Variant.
S. Yavuz, A. S. Yavuz, K. H. Kraemer, and P. E. Lipsky (2002)
J. Immunol. 169, 3825-3830
   Abstract »    Full Text »    PDF »
Translesion DNA synthesis in eukaryotes: A one- or two-polymerase affair.
S. Prakash and L. Prakash (2002)
Genes & Dev. 16, 1872-1883
   Full Text »    PDF »
DNA damage responses protect xeroderma pigmentosum variant from UVC-induced clastogenesis.
M. Cordeiro-Stone, A. Frank, M. Bryant, I. Oguejiofor, S. B. Hatch, L. D. McDaniel, and W. K. Kaufmann (2002)
Carcinogenesis 23, 959-966
   Abstract »    Full Text »    PDF »
Low Fidelity DNA Synthesis by a Y Family DNA Polymerase Due to Misalignment in the Active Site.
R. J. Kokoska, K. Bebenek, F. Boudsocq, R. Woodgate, and T. A. Kunkel (2002)
J. Biol. Chem. 277, 19633-19638
   Abstract »    Full Text »    PDF »
Human DNA polymerase mu (Pol {micro}) exhibits an unusual replication slippage ability at AAF lesion.
J.-B. Duvauchelle, L. Blanco, R. P. P. Fuchs, and A. M. Cordonnier (2002)
Nucleic Acids Res. 30, 2061-2067
   Abstract »    Full Text »    PDF »
Posttranslational modification of the umuD-encoded subunit of Escherichia coli DNA polymerase V regulates its interactions with the beta processivity clamp.
M. D. Sutton, I. Narumi, and G. C. Walker (2002)
PNAS 99, 5307-5312
   Abstract »    Full Text »    PDF »
Response of human REV1 to different DNA damage: preferential dCMP insertion opposite the lesion.
Y. Zhang, X. Wu, O. Rechkoblit, N. E. Geacintov, J.-S. Taylor, and Z. Wang (2002)
Nucleic Acids Res. 30, 1630-1638
   Abstract »    Full Text »    PDF »
Preferential Misincorporation of Purine Nucleotides by Human DNA Polymerase eta Opposite Benzo[a]pyrene 7,8-Diol 9,10-Epoxide Deoxyguanosine Adducts.
D. Chiapperino, H. Kroth, I. H. Kramarczuk, J. M. Sayer, C. Masutani, F. Hanaoka, D. M. Jerina, and A. M. Cheh (2002)
J. Biol. Chem. 277, 11765-11771
   Abstract »    Full Text »    PDF »
Quantitative measurement of translesion replication in human cells: Evidence for bypass of abasic sites by a replicative DNA polymerase.
S. Avkin, S. Adar, G. Blander, and Z. Livneh (2002)
PNAS 99, 3764-3769
   Abstract »    Full Text »    PDF »
UV-induced T->C transition at a TT photoproduct site is dependent on Saccharomyces cerevisiae polymerase {eta}in vivo.
H. Zhang and W. Siede (2002)
Nucleic Acids Res. 30, 1262-1267
   Abstract »    Full Text »    PDF »
Stimulation of DNA Synthesis Activity of Human DNA Polymerase {kappa} by PCNA.
L. Haracska, I. Unk, R. E. Johnson, B. B. Phillips, J. Hurwitz, L. Prakash, and S. Prakash (2002)
Mol. Cell. Biol. 22, 784-791
   Abstract »    Full Text »    PDF »
Molecular analysis of mutations in DNA polymerase eta in xeroderma pigmentosum-variant patients.
B. C. Broughton, A. Cordonnier, W. J. Kleijer, N. G. J. Jaspers, H. Fawcett, A. Raams, V. H. Garritsen, A. Stary, M.-F. Avril, F. Boudsocq, et al. (2002)
PNAS
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)