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Science 18 June 1999:
Vol. 284. no. 5422, pp. 1976 - 1979
DOI: 10.1126/science.284.5422.1976
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Reports

Xyloglucan Fucosyltransferase, an Enzyme Involved in Plant Cell Wall Biosynthesis

Robyn M. Perrin, 12* Amy E. DeRocher, 1* Maor Bar-Peled, 1*dagger Weiqing Zeng, 14 Lorena Norambuena, 5 Ariel Orellana, 5 Natasha V. Raikhel, 13ddagger Kenneth Keegstra 123ddagger

Cell walls are crucial for development, signal transduction, and disease resistance in plants. Cell walls are made of cellulose, hemicelluloses, and pectins. Xyloglucan (XG), the principal load-bearing hemicellulose of dicotyledonous plants, has a terminal fucosyl residue. A 60-kilodalton fucosyltransferase (FTase) that adds this residue was purified from pea epicotyls. Peptide sequence information from the pea FTase allowed the cloning of a homologous gene, AtFT1, from Arabidopsis. Antibodies raised against recombinant AtFTase immunoprecipitate FTase enzyme activity from solubilized Arabidopsis membrane proteins, and AtFT1 expressed in mammalian COS cells results in the presence of XG FTase activity in these cells.

1 Michigan State University-Department of Energy (MSU-DOE) Plant Research Laboratory,
2 Department of Botany and Plant Pathology,
3 Department of Biochemistry,
4 Cell and Molecular Biology Program, Michigan State University, East Lansing, MI 48824, USA.
5 Department of Biology, Faculty of Sciences, University of Chile, Santiago, Chile.
*   These authors contributed equally to this work.

dagger    Present address: Monsanto, St. Louis, MO 63198, USA.

ddagger    To whom correspondence should be addressed at MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, MI 48824, USA. E-mail: nraikhel{at}pilot.msu.edu (N.V.R.); keegstra{at}pilot.msu.edu (K.K.)

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