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Science 28 May 1999:
Vol. 284. no. 5419, pp. 1527 - 1530
DOI: 10.1126/science.284.5419.1527
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Reports

Spatiotemporal Dynamics of Inositol 1,4,5-Trisphosphate That Underlies Complex Ca2+ Mobilization Patterns

Kenzo Hirose, * Shiro Kadowaki, Mao Tanabe, Hiroshi Takeshima, Masamitsu Iino

Inositol 1,4,5-trisphosphate (IP3) is a second messenger that elicits complex spatiotemporal patterns of calcium ion (Ca2+) mobilization and has essential roles in the regulation of many cellular functions. In Madin-Darby canine kidney epithelial cells, green fluorescent protein-tagged pleckstrin homology domain translocated from the plasma membrane to the cytoplasm in response to increased concentration of IP3. The detection of translocation enabled monitoring of IP3 concentration changes within single cells and revealed spatiotemporal dynamics in the concentration of IP3 synchronous with Ca2+ oscillations and intracellular and intercellular IP3 waves that accompanied Ca2+ waves. Such changes in IP3 concentration may be fundamental to Ca2+ signaling.

Department of Pharmacology, Faculty of Medicine, University of Tokyo and CREST, Japan Science and Technology Corporation, Tokyo 113-8654, Japan.
*   To whom correspondence to be addressed. E-mail: hirose{at}calcium.cmp.m.u-tokyo.ac.jp

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J.-Y. Jung, Y.-W. Kim, J. M. Kwak, J.-U. Hwang, J. Young, J. I. Schroeder, I. Hwang, and Y. Lee (2002)
PLANT CELL 14, 2399-2412
   Abstract »    Full Text »    PDF »
Determinants of Metabotropic Glutamate Receptor-5-mediated Ca2+ and Inositol 1,4,5-Trisphosphate Oscillation Frequency. RECEPTOR DENSITY VERSUS AGONIST CONCENTRATION.
M. S. Nash, M. J. Schell, P. J. Atkinson, N. R. Johnston, S. R. Nahorski, and R. A. J. Challiss (2002)
J. Biol. Chem. 277, 35947-35960
   Abstract »    Full Text »    PDF »
Calcium wave pacemakers in eggs.
R. Dumollard, J. Carroll, G. Dupont, and C. Sardet (2002)
J. Cell Sci. 115, 3557-3564
   Abstract »    Full Text »    PDF »
Spatial Segregation and Interaction of Calcium Signalling Mechanisms in Rat Hippocampal CA1 Pyramidal Neurons.
T. Nakamura, N. Lasser-Ross, K. Nakamura, and W. N Ross (2002)
J. Physiol. 543, 465-480
   Abstract »    Full Text »    PDF »
Patterns of intracellular and intercellular Ca2+ waves in the longitudinal muscle layer of the murine large intestine In vitro.
G. W Hennig, C. B Smith, D. M O'Shea, and T. K Smith (2002)
J. Physiol. 543, 233-253
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TAPP1 and TAPP2 Are Targets of Phosphatidylinositol 3-Kinase Signaling in B Cells: Sustained Plasma Membrane Recruitment Triggered by the B-Cell Antigen Receptor.
A. J. Marshall, A. K. Krahn, K. Ma, V. Duronio, and S. Hou (2002)
Mol. Cell. Biol. 22, 5479-5491
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Localized Ca2+ uncaging reveals polarized distribution of Ca2+-sensitive Ca2+ release sites: mechanism of unidirectional Ca2+ waves.
M. C. Ashby, M. Craske, M. K. Park, O. V. Gerasimenko, R. D. Burgoyne, O. H. Petersen, and A. V. Tepikin (2002)
J. Cell Biol. 158, 283-292
   Abstract »    Full Text »    PDF »
Inositol Lipid Binding and Membrane Localization of Isolated Pleckstrin Homology (PH) Domains. STUDIES ON THE PH DOMAINS OF PHOSPHOLIPASE C delta 1 AND p130.
P. Varnai, X. Lin, S. B. Lee, G. Tuymetova, T. Bondeva, A. Spat, S. G. Rhee, G. Hajnoczky, and T. Balla (2002)
J. Biol. Chem. 277, 27412-27422
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Polarized Calcium and Calmodulin Signaling in Secretory Epithelia.
M. C. Ashby and A. V. Tepikin (2002)
Physiol Rev 82, 701-734
   Abstract »    Full Text »    PDF »
Modulation of cardiac PIP2 by cardioactive hormones and other physiologically relevant interventions.
C. Nasuhoglu, S. Feng, Y. Mao, I. Shammat, M. Yamamato, S. Earnest, M. Lemmon, and D. W. Hilgemann (2002)
Am J Physiol Cell Physiol 283, C223-C234
   Abstract »    Full Text »    PDF »
The dynamics of plasma membrane PtdIns(4,5)P2 at fertilization of mouse eggs.
G. Halet, R. Tunwell, T. Balla, K. Swann, and J. Carroll (2002)
J. Cell Sci. 115, 2139-2149
   Abstract »    Full Text »    PDF »


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