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Science 21 May 1999:
Vol. 284. no. 5418, pp. 1372 - 1376
DOI: 10.1126/science.284.5418.1372
Prev | Table of Contents

Reports

UDP-GlcNAc 2-Epimerase: A Regulator of Cell Surface Sialylation

Oliver T. Keppler, 1*dagger Stephan Hinderlich, 3* Josmar Langner, 1 Reinhard Schwartz-Albiez, 2 Werner Reutter, 3 Michael Pawlita 1ddagger

Modification of cell surface molecules with sialic acid is crucial for their function in many biological processes, including cell adhesion and signal transduction. Uridine diphosphate-N-acetylglucosamine 2-epimerase (UDP-GlcNAc 2-epimerase) is an enzyme that catalyzes an early, rate-limiting step in the sialic acid biosynthetic pathway. UDP-GlcNAc 2-epimerase was found to be a major determinant of cell surface sialylation in human hematopoietic cell lines and a critical regulator of the function of specific cell surface adhesion molecules.

1 Applied Tumor Virology Program,
2 Tumor Immunology Program, Deutsches Krebsforschungszentrum, Im Neuenheimer Feld 280, D-69120 Heidelberg, Germany.
3 Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-14195 Berlin-Dahlem, Germany.
*   These authors contributed equally to this work.

dagger    Present address: Gladstone Institute of Virology and Immunology, Post Office Box 419100, San Francisco, CA 94141-9100, USA.

ddagger    To whom correspondence should be addressed. E-mail: M.Pawlita{at}dkfz-heidelberg.de

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Science. ISSN 0036-8075 (print), 1095-9203 (online)