Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 21 May 1999:
Vol. 284. no. 5418, pp. 1359 - 1362
DOI: 10.1126/science.284.5418.1359
Prev | Table of Contents | Next

Reports

Stimulation of Microtubule Aster Formation and Spindle Assembly by the Small GTPase Ran

Andrew Wilde, Yixian Zheng *

Ran, a small guanosine triphosphatase, is suggested to have additional functions beyond its well-characterized role in nuclear trafficking. Guanosine triphosphate-bound Ran, but not guanosine diphosphate-bound Ran, stimulated polymerization of astral microtubules from centrosomes assembled on Xenopus sperm. Moreover, a Ran allele with a mutation in the effector domain (RanL43E) induced the formation of microtubule asters and spindle assembly, in the absence of sperm nuclei, in a gamma TuRC (gamma -tubulin ring complex)- and XMAP215 (Xenopus microtubule associated protein)-dependent manner. Therefore, Ran could be a key signaling molecule regulating microtubule polymerization during mitosis.

Carnegie Institution of Washington, Department of Embryology, Baltimore, MD 21210, USA.
*   To whom correspondence should be addressed. E-mail: zheng{at}mail1.ciwemb.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
{gamma}-Tubulin ring complexes regulate microtubule plus end dynamics.
A. Bouissou, C. Verollet, A. Sousa, P. Sampaio, M. Wright, C. E. Sunkel, A. Merdes, and B. Raynaud-Messina (2009)
J. Cell Biol. 187, 327-334
   Abstract »    Full Text »    PDF »
Learning about cancer from frogs: analysis of mitotic spindles in Xenopus egg extracts.
M. K. Cross and M. A. Powers (2009)
Dis. Model. Mech. 2, 541-547
   Abstract »    Full Text »    PDF »
Interaction between Poly(ADP-ribose) and NuMA Contributes to Mitotic Spindle Pole Assembly.
P. Chang, M. Coughlin, and T. J. Mitchison (2009)
Mol. Biol. Cell 20, 4575-4585
   Abstract »    Full Text »    PDF »
Transportin Regulates Major Mitotic Assembly Events: From Spindle to Nuclear Pore Assembly.
C. K. Lau, V. A. Delmar, R. C. Chan, Q. Phung, C. Bernis, B. Fichtman, B. A. Rasala, and D. J. Forbes (2009)
Mol. Biol. Cell 20, 4043-4058
   Abstract »    Full Text »    PDF »
XMAP215-EB1 Interaction Is Required for Proper Spindle Assembly and Chromosome Segregation in Xenopus Egg Extract.
I. Kronja, A. Kruljac-Letunic, M. Caudron-Herger, P. Bieling, and E. Karsenti (2009)
Mol. Biol. Cell 20, 2684-2696
   Abstract »    Full Text »    PDF »
Dynamic release of nuclear RanGTP triggers TPX2-dependent microtubule assembly during the apoptotic execution phase.
D. K. Moss, A. Wilde, and J. D. Lane (2009)
J. Cell Sci. 122, 644-655
   Abstract »    Full Text »    PDF »
Kinesin-14 Family Proteins HSET/XCTK2 Control Spindle Length by Cross-Linking and Sliding Microtubules.
S. Cai, L. N. Weaver, S. C. Ems-McClung, and C. E. Walczak (2009)
Mol. Biol. Cell 20, 1348-1359
   Abstract »    Full Text »    PDF »
Mars, a Drosophila protein related to vertebrate HURP, is required for the attachment of centrosomes to the mitotic spindle during syncytial nuclear divisions.
G. Zhang, M. Breuer, A. Forster, D. Egger-Adam, and A. Wodarz (2009)
J. Cell Sci. 122, 535-545
   Abstract »    Full Text »    PDF »
FAM29A promotes microtubule amplification via recruitment of the NEDD1-{gamma}-tubulin complex to the mitotic spindle.
H. Zhu, J. A. Coppinger, C.-Y. Jang, J. R. Yates III, and G. Fang (2008)
J. Cell Biol. 183, 835-848
   Abstract »    Full Text »    PDF »
Hepatoma Up-Regulated Protein Is Required for Chromatin-induced Microtubule Assembly Independently of TPX2.
C. M. Casanova, S. Rybina, H. Yokoyama, E. Karsenti, and I. W. Mattaj (2008)
Mol. Biol. Cell 19, 4900-4908
   Abstract »    Full Text »    PDF »
Aurora A Phosphorylates MCAK to Control Ran-dependent Spindle Bipolarity.
X. Zhang, S. C. Ems-McClung, and C. E. Walczak (2008)
Mol. Biol. Cell 19, 2752-2765
   Abstract »    Full Text »    PDF »
Regulation of Microtubule Assembly and Organization in Mitosis by the AAA+ ATPase Pontin.
D. Ducat, S.-i. Kawaguchi, H. Liu, J. R. Yates III, and Y. Zheng (2008)
Mol. Biol. Cell 19, 3097-3110
   Abstract »    Full Text »    PDF »
Serial analysis of the vascular endothelial transcriptome under static and shear stress conditions.
T. J. Chu and D. G. Peters (2008)
Physiol Genomics 34, 185-192
   Abstract »    Full Text »    PDF »
RanBP10 Is a Cytoplasmic Guanine Nucleotide Exchange Factor That Modulates Noncentrosomal Microtubules.
H. Schulze, M. Dose, M. Korpal, I. Meyer, J. E. Italiano Jr., and R. A. Shivdasani (2008)
J. Biol. Chem. 283, 14109-14119
   Abstract »    Full Text »    PDF »
Cdk11 is a RanGTP-dependent microtubule stabilization factor that regulates spindle assembly rate.
H. Yokoyama, O. J. Gruss, S. Rybina, M. Caudron, M. Schelder, M. Wilm, I. W. Mattaj, and E. Karsenti (2008)
J. Cell Biol. 180, 867-875
   Abstract »    Full Text »    PDF »
Xenopus NEDD1 is required for microtubule organization in Xenopus egg extracts.
L. Liu and C. Wiese (2008)
J. Cell Sci. 121, 578-589
   Abstract »    Full Text »    PDF »
Aurora B Phosphorylates Multiple Sites on Mitotic Centromere-associated Kinesin to Spatially and Temporally Regulate Its Function.
X. Zhang, W. Lan, S. C. Ems-McClung, P. T. Stukenberg, and C. E. Walczak (2007)
Mol. Biol. Cell 18, 3264-3276
   Abstract »    Full Text »    PDF »
An in vitro nuclear disassembly system reveals a role for the RanGTPase system and microtubule-dependent steps in nuclear envelope breakdown.
P. Muhlhausser and U. Kutay (2007)
J. Cell Biol. 178, 595-610
   Abstract »    Full Text »    PDF »
Diazonamide toxins reveal an unexpected function for ornithine {delta}-amino transferase in mitotic cell division.
G. Wang, L. Shang, A. W. G. Burgett, P. G. Harran, and X. Wang (2007)
PNAS 104, 2068-2073
   Abstract »    Full Text »    PDF »
A centriole- and RanGTP-independent spindle assembly pathway in meiosis I of vertebrate oocytes.
J. Dumont, S. Petri, F. Pellegrin, M.-E. Terret, M. T. Bohnsack, P. Rassinier, V. Georget, P. Kalab, O. J. Gruss, and M.-H. Verlhac (2007)
J. Cell Biol. 176, 295-305
   Abstract »    Full Text »    PDF »
The Interplay of the N- and C-Terminal Domains of MCAK Control Microtubule Depolymerization Activity and Spindle Assembly.
S. C. Ems-McClung, K. M. Hertzer, X. Zhang, M. W. Miller, and C. E. Walczak (2007)
Mol. Biol. Cell 18, 282-294
   Abstract »    Full Text »    PDF »
Phosphorylation of Maskin by Aurora-A Is Regulated by RanGTP and Importin beta.
A. J. Albee, W. Tao, and C. Wiese (2006)
J. Biol. Chem. 281, 38293-38301
   Abstract »    Full Text »    PDF »
Noncore Components of the Fission Yeast {gamma}-Tubulin Complex.
A. Anders, P. C.C. Lourenco, and K. E. Sawin (2006)
Mol. Biol. Cell 17, 5075-5093
   Abstract »    Full Text »    PDF »
The Nup107-160 Nucleoporin Complex Is Required for Correct Bipolar Spindle Assembly.
A. V. Orjalo, A. Arnaoutov, Z. Shen, Y. Boyarchuk, S. G. Zeitlin, B. Fontoura, S. Briggs, M. Dasso, and D. J. Forbes (2006)
Mol. Biol. Cell 17, 3806-3818
   Abstract »    Full Text »    PDF »
A picornavirus protein interacts with Ran-GTPase and disrupts nucleocytoplasmic transport.
F. W. Porter, Y. A. Bochkov, A. J. Albee, C. Wiese, and A. C. Palmenberg (2006)
PNAS 103, 12417-12422
   Abstract »    Full Text »    PDF »
NuSAP, a Mitotic RanGTP Target That Stabilizes and Cross-links Microtubules.
K. Ribbeck, A. C. Groen, R. Santarella, M. T. Bohnsack, T. Raemaekers, T. Kocher, M. Gentzel, D. Gorlich, M. Wilm, G. Carmeliet, et al. (2006)
Mol. Biol. Cell 17, 2646-2660
   Abstract »    Full Text »    PDF »
CLIP-170 Homologue and NUDE Play Overlapping Roles in NUDF Localization in Aspergillus nidulans.
V. P. Efimov, J. Zhang, and X. Xiang (2006)
Mol. Biol. Cell 17, 2021-2034
   Abstract »    Full Text »    PDF »
Ran Is Required before Metaphase for Spindle Assembly and Chromosome Alignment and after Metaphase for Chromosome Segregation and Spindle Midbody Organization.
R. V. Silverman-Gavrila and A. Wilde (2006)
Mol. Biol. Cell 17, 2069-2080
   Abstract »    Full Text »    PDF »
A Mitotic Lamin B Matrix Induced by RanGTP Required for Spindle Assembly.
M.-Y. Tsai, S. Wang, J. M. Heidinger, D. K. Shumaker, S. A. Adam, R. D. Goldman, and Y. Zheng (2006)
Science 311, 1887-1893
   Abstract »    Full Text »    PDF »
Aurora B is required for mitotic chromatin-induced phosphorylation of Op18/Stathmin.
B. B. Gadea and J. V. Ruderman (2006)
PNAS 103, 4493-4498
   Abstract »    Full Text »    PDF »
Comparative and evolutionary analysis of genes encoding small GTPases and their activating proteins in eukaryotic genomes.
S.-Y. Jiang and S. Ramachandran (2006)
Physiol Genomics 24, 235-251
   Abstract »    Full Text »    PDF »
Overexpression of RAN1 in Rice and Arabidopsis Alters Primordial Meristem, Mitotic Progress, and Sensitivity to Auxin.
X. Wang, Y. Xu, Y. Han, S. Bao, J. Du, M. Yuan, Z. Xu, and K. Chong (2006)
Plant Physiology 140, 91-101
   Abstract »    Full Text »    PDF »
Anaphase-Promoting Complex/Cyclosome Controls the Stability of TPX2 during Mitotic Exit.
S. Stewart and G. Fang (2005)
Mol. Cell. Biol. 25, 10516-10527
   Abstract »    Full Text »    PDF »
Measuring the Stoichiometry and Physical Interactions between Components Elucidates the Architecture of the Vertebrate Kinetochore.
M. J. Emanuele, M. L. McCleland, D. L. Satinover, and P. T. Stukenberg (2005)
Mol. Biol. Cell 16, 4882-4892
   Abstract »    Full Text »    PDF »
Spatial Coordination of Spindle Assembly by Chromosome-Mediated Signaling Gradients.
M. Caudron, G. Bunt, P. Bastiaens, and E. Karsenti (2005)
Science 309, 1373-1376
   Abstract »    Full Text »    PDF »
The Xenopus TACC Homologue, Maskin, Functions in Mitotic Spindle Assembly.
L. L. O'Brien, A. J. Albee, L. Liu, W. Tao, P. Dobrzyn, S. B. Lizarraga, and C. Wiese (2005)
Mol. Biol. Cell 16, 2836-2847
   Abstract »    Full Text »    PDF »
A {beta}-Tubulin Mutation Selectively Uncouples Nuclear Division and Cytokinesis in Tetrahymena thermophila.
J. J. Smith, J. S. Yakisich, G. M. Kapler, E. S. Cole, and D. P. Romero (2004)
Eukaryot. Cell 3, 1217-1226
   Abstract »    Full Text »    PDF »
The mechanism of spindle assembly: functions of Ran and its target TPX2.
O. J. Gruss and I. Vernos (2004)
J. Cell Biol. 166, 949-955
   Abstract »    Full Text »    PDF »
Mitosis-specific Anchoring of {gamma} Tubulin Complexes by Pericentrin Controls Spindle Organization and Mitotic Entry.
W. C. Zimmerman, J. Sillibourne, J. Rosa, and S. J. Doxsey (2004)
Mol. Biol. Cell 15, 3642-3657
   Abstract »    Full Text »    PDF »
Freeze-Dried Sperm Fertilization Leads to Full-Term Development in Rabbits.
J.-L. Liu, H. Kusakabe, C.-C. Chang, H. Suzuki, D. W. Schmidt, M. Julian, R. Pfeffer, C. L. Bormann, X. C. Tian, R. Yanagimachi, et al. (2004)
Biol Reprod 70, 1776-1781
   Abstract »    Full Text »    PDF »
Differentiation of Cytoplasmic and Meiotic Spindle Assembly MCAK Functions by Aurora B-dependent Phosphorylation.
R. Ohi, T. Sapra, J. Howard, and T. J. Mitchison (2004)
Mol. Biol. Cell 15, 2895-2906
   Abstract »    Full Text »    PDF »
TOGp, the Human Homolog of XMAP215/Dis1, Is Required for Centrosome Integrity, Spindle Pole Organization, and Bipolar Spindle Assembly.
L. Cassimeris and J. Morabito (2004)
Mol. Biol. Cell 15, 1580-1590
   Abstract »    Full Text »    PDF »
Phosphorylation of RCC1 in mitosis is essential for producing a high RanGTP concentration on chromosomes and for spindle assembly in mammalian cells.
H.-Y. Li and Y. Zheng (2004)
Genes & Dev. 18, 512-527
   Abstract »    Full Text »    PDF »
Importin {alpha}/{beta} and Ran-GTP Regulate XCTK2 Microtubule Binding through a Bipartite Nuclear Localization Signal.
S. C. Ems-McClung, Y. Zheng, and C. E. Walczak (2004)
Mol. Biol. Cell 15, 46-57
   Abstract »    Full Text »    PDF »
Ran modulates spindle assembly by regulating a subset of TPX2 and Kid activities including Aurora A activation.
N. Trieselmann, S. Armstrong, J. Rauw, and A. Wilde (2003)
J. Cell Sci. 116, 4791-4798
   Abstract »    Full Text »    PDF »
Biochemical Characterization of the Ran-RanBP1-RanGAP System: Are RanBP Proteins and the Acidic Tail of RanGAP Required for the Ran-RanGAP GTPase Reaction?.
M. J. Seewald, A. Kraemer, M. Farkasovsky, C. Korner, A. Wittinghofer, and I. R. Vetter (2003)
Mol. Cell. Biol. 23, 8124-8136
   Abstract »    Full Text »    PDF »
Regulated Expression of the Centrosomal Protein DdCP224 Affects Microtubule Dynamics and Reveals Mechanisms for the Control of Supernumerary Centrosome Number.
R. Graf, U. Euteneuer, T.-H. Ho, and M. Rehberg (2003)
Mol. Biol. Cell 14, 4067-4074
   Abstract »    Full Text »    PDF »
Part of Ran Is Associated with AKAP450 at the Centrosome: Involvement in Microtubule-organizing Activity.
G. Keryer, B. Di Fiore, C. Celati, K. F. Lechtreck, M. Mogensen, A. Delouvee, P. Lavia, M. Bornens, and A.-M. Tassin (2003)
Mol. Biol. Cell 14, 4260-4271
   Abstract »    Full Text »    PDF »
The maternal-effect gene futile cycle is essential for pronuclear congression and mitotic spindle assembly in the zebrafish zygote.
M. P. S. Dekens, F. J. Pelegri, H.-M. Maischein, and C. Nusslein-Volhard (2003)
Development 130, 3907-3916
   Abstract »    Full Text »    PDF »
Mammalian RanBP1 regulates centrosome cohesion during mitosis.
B. Di Fiore, M. Ciciarello, R. Mangiacasale, A. Palena, A.-M. Tassin, E. Cundari, and P. Lavia (2003)
J. Cell Sci. 116, 3399-3411
   Abstract »    Full Text »    PDF »
Involvement of Crm1 in Hepatitis B Virus X Protein-Induced Aberrant Centriole Replication and Abnormal Mitotic Spindles.
M. Forgues, M. J. Difilippantonio, S. P. Linke, T. Ried, K. Nagashima, J. Feden, K. Valerie, K. Fukasawa, and X. W. Wang (2003)
Mol. Cell. Biol. 23, 5282-5292
   Abstract »    Full Text »    PDF »
Spindle Formation in Aspergillus Is Coupled to Tubulin Movement into the Nucleus.
Y. Ovechkina, P. Maddox, C. E. Oakley, X. Xiang, S. A. Osmani, E. D. Salmon, and B. R. Oakley (2003)
Mol. Biol. Cell 14, 2192-2200
   Abstract »    Full Text »    PDF »
The Ran GTPase cycle is required for yeast nuclear pore complex assembly.
K. J. Ryan, J. M. McCaffery, and S. R. Wente (2003)
J. Cell Biol. 160, 1041-1053
   Abstract »    Full Text »    PDF »
A mechanism of coupling RCC1 mobility to RanGTP production on the chromatin in vivo.
H. Y. Li, D. Wirtz, and Y. Zheng (2003)
J. Cell Biol. 160, 635-644
   Abstract »    Full Text »    PDF »
Analysis of the Small GTPase Gene Superfamily of Arabidopsis.
V. Vernoud, A. C. Horton, Z. Yang, and E. Nielsen (2003)
Plant Physiology 131, 1191-1208
   Abstract »    Full Text »    PDF »
Ran GTPase Cycle and Importins alpha and beta Are Essential for Spindle Formation and Nuclear Envelope Assembly in Living Caenorhabditis elegans Embryos.
P. Askjaer, V. Galy, E. Hannak, and I. W. Mattaj (2002)
Mol. Biol. Cell 13, 4355-4370
   Abstract »    Full Text »    PDF »
Visualization of a Ran-GTP Gradient in Interphase and Mitotic Xenopus Egg Extracts.
P. Kalab, K. Weis, and R. Heald (2002)
Science 295, 2452-2456
   Abstract »    Full Text »    PDF »
XMAP215 is a long thin molecule that does not increase microtubule stiffness.
L. Cassimeris, D. Gard, P. T. Tran, and H. P. Erickson (2002)
J. Cell Sci. 114, 3025-3033
   Abstract »    Full Text »    PDF »
KRP3A and KRP3B: Candidate Motors in Spermatid Maturation in the Seminiferous Epithelium.
Y. Zou, C. F. Millette, and A. O. Sperry (2002)
Biol Reprod 66, 843-855
   Abstract »    Full Text »    PDF »
Chromosomal association of Ran during meiotic and mitotic divisions.
B. Hinkle, B. Slepchenko, M. M. Rolls, T. C. Walther, P. A. Stein, L. M. Mehlmann, J. Ellenberg, and M. Terasaki (2002)
J. Cell Sci. 115, 4685-4693
   Abstract »    Full Text »    PDF »
Katanin inhibition prevents the redistribution of {gamma}-tubulin at mitosis.
D. Buster, K. McNally, and F. J. McNally (2002)
J. Cell Sci. 115, 1083-1092
   Abstract »    Full Text »    PDF »
Transport into and out of the Nucleus.
I. G. Macara (2001)
Microbiol. Mol. Biol. Rev. 65, 570-594
   Abstract »    Full Text »    PDF »
Role of Importin-beta in Coupling Ran to Downstream Targets in Microtubule Assembly.
C. Wiese, A. Wilde, M. S. Moore, S. A. Adam, A. Merdes, and Y. Zheng (2001)
Science 291, 653-656
   Abstract »    Full Text »
Dis1/TOG universal microtubule adaptors - one MAP for all?.
H. Ohkura, M. A. Garcia, and T. Toda (2001)
J. Cell Sci. 114, 3805-3812
   Abstract »    Full Text »    PDF »
Small GTP-Binding Proteins.
Y. Takai, T. Sasaki, and T. Matozaki (2001)
Physiol Rev 81, 153-208
   Abstract »    Full Text »    PDF »
The Fission Yeast Ran Gtpase Is Required for Microtubule Integrity.
U. Fleig, S. S. Salus, I. Karig, and S. Sazer (2000)
J. Cell Biol. 151, 1101-1112
   Abstract »    Full Text »    PDF »
Regulated Ran-binding Protein 1 Activity Is Required for Organization and Function of the Mitotic Spindle in Mammalian Cells in Vivo.
G. Guarguaglini, L. Renzi, F. D’Ottavio, B. Di Fiore, M. Casenghi, E. Cundari, and P. Lavia (2000)
Cell Growth Differ. 11, 455-465
   Abstract »    Full Text »
The Interaction Between Ran and NTF2 is Required for Cell Cycle Progression.
B. B. Quimby, C. A. Wilson, and A. H. Corbett (2000)
Mol. Biol. Cell 11, 2617-2629
   Abstract »    Full Text »
Tpx2, a Novel Xenopus Map Involved in Spindle Pole Organization.
T. Wittmann, M. Wilm, E. Karsenti, and I. Vernos (2000)
J. Cell Biol. 149, 1405-1418
   Abstract »    Full Text »    PDF »
Chromatin-Independent Nuclear Envelope Assembly Induced by Ran GTPase in Xenopus Egg Extracts.
C. Zhang and P. R. Clarke (2000)
Science 288, 1429-1432
   Abstract »    Full Text »
An Adenovirus Inhibitor of Tumor Necrosis Factor Alpha-Induced Apoptosis Complexes with Dynein and a Small GTPase.
S. A. Lukashok, L. Tarassishin, Y. Li, and M. S. Horwitz (2000)
J. Virol. 74, 4705-4709
   Abstract »    Full Text »
Nuclear Import of the Ran Exchange Factor, Rcc1, Is Mediated by at Least Two Distinct Mechanisms.
M. E. Nemergut and I. G. Macara (2000)
J. Cell Biol. 149, 835-850
   Abstract »    Full Text »    PDF »
The Nuclear Import of RCC1 Requires a Specific Nuclear Localization Sequence Receptor, Karyopherin alpha 3/Qip.
B. Talcott and M. S. Moore (2000)
J. Biol. Chem. 275, 10099-10104
   Abstract »    Full Text »    PDF »
Random Mutagenesis and Functional Analysis of the Ran-binding Protein, RanBP1.
C. Petersen, N. Orem, J. Trueheart, J. W. Thorner, and I. G. Macara (2000)
J. Biol. Chem. 275, 4081-4091
   Abstract »    Full Text »    PDF »
Biochemical and Genetic Conservation of Fission Yeast Dsk1 and Human SR Protein-Specific Kinase 1.
Z. Tang, T. Kuo, J. Shen, and R.-J. Lin (2000)
Mol. Cell. Biol. 20, 816-824
   Abstract »    Full Text »
Purification of Protein A-tagged Yeast Ran Reveals Association with a Novel Karyopherin beta Family Member, Pdr6p.
D. Lau, M. Kunzler, A. Braunwarth, K. Hellmuth, A. Podtelejnikov, M. Mann, and E. Hurt (2000)
J. Biol. Chem. 275, 467-471
   Abstract »    Full Text »    PDF »
The ran decathlon: multiple roles of Ran.
S Sazer and M Dasso (2000)
J. Cell Sci. 113, 1111-1118
   Abstract »    PDF »
Beyond Nuclear Transport: Ran-Gtp as a Determinant of Spindle Assembly.
J. A. Kahana and D. W. Cleveland (1999)
J. Cell Biol. 146, 1205-1210
   Full Text »    PDF »
BSAP (Pax5)-Importin alpha 1 (Rch1) Interaction Identifies a Nuclear Localization Sequence.
C. R. Kovac, A. Emelyanov, M. Singh, N. Ashouian, and B. K. Birshtein (2000)
J. Biol. Chem. 275, 16752-16757
   Abstract »    Full Text »    PDF »
The Interaction of TOGp with Microtubules and Tubulin.
C. Spittle, S. Charrasse, C. Larroque, and L. Cassimeris (2000)
J. Biol. Chem. 275, 20748-20753
   Abstract »    Full Text »    PDF »
Novel G Proteins, Rag C and Rag D, Interact with GTP-binding Proteins, Rag A and Rag B.
T. Sekiguchi, E. Hirose, N. Nakashima, M. Ii, and T. Nishimoto (2001)
J. Biol. Chem. 276, 7246-7257
   Abstract »    Full Text »    PDF »
Nuclear Import of the U1A Splicesome Protein Is Mediated by Importin alpha /beta and Ran in Living Mammalian Cells.
M. Hieda, T. Tachibana, M. Fukumoto, and Y. Yoneda (2001)
J. Biol. Chem. 276, 16824-16832
   Abstract »    Full Text »    PDF »
The Mitotic Checkpoint Protein hBUB3 and the mRNA Export Factor hRAE1 Interact with GLE2p-binding Sequence (GLEBS)-containing Proteins.
X. Wang, J. R. Babu, J. M. Harden, S. A. Jablonski, M. H. Gazi, W. L. Lingle, P. C. de Groen, T. J. Yen, and J. M. A. van Deursen (2001)
J. Biol. Chem. 276, 26559-26567
   Abstract »    Full Text »    PDF »
Interaction of the Hepatitis B Virus X Protein with the Crm1-dependent Nuclear Export Pathway.
M. Forgues, A. J. Marrogi, E. A. Spillare, C.-G. Wu, Q. Yang, M. Yoshida, and X. W. Wang (2001)
J. Biol. Chem. 276, 22797-22803
   Abstract »    Full Text »    PDF »
A Single Point Mutation at the 3'-Untranslated Region of Ran mRNA Leads to Profound Changes in Lipopolysaccharide Endotoxin-mediated Responses.
P. M. C. Wong, Q. Yuan, H. Chen, B. M. Sultzer, and S.-W. Chung (2001)
J. Biol. Chem. 276, 33129-33138
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)