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Science 21 May 1999:
Vol. 284. no. 5418, pp. 1356 - 1358
DOI: 10.1126/science.284.5418.1356
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Reports

Self-Organization of Microtubule Asters Induced in Xenopus Egg Extracts by GTP-Bound Ran

T. Ohba, M. Nakamura, H. Nishitani, T. Nishimoto *

The nucleotide exchange activity of RCC1, the only known nucleotide exchange factor for Ran, a Ras-like small guanosine triphosphatase, was required for microtubule aster formation with or without demembranated sperm in Xenopus egg extracts arrested in meiosis II. Consistently, in the RCC1-depleted egg extracts, Ran guanosine triphosphate (RanGTP), but not Ran guanosine diphosphate (RanGDP), induced self-organization of microtubule asters, and the process required the activity of dynein. Thus, Ran was shown to regulate formation of the microtubule network.

Department of Molecular Biology, Graduate School of Medical Science, Kyushu University, Maidashi Higashi-ku, Fukuoka 812-8582, Japan.
*   To whom correspondence should be addressed. E-mail: tnishi{at}molbiol.med.kyushu-u.ac.jp

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