Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 26 March 1999:
Vol. 283. no. 5410, pp. 2097 - 2100
DOI: 10.1126/science.283.5410.2097
Prev | Table of Contents

Reports

A Cytotoxic Ribonuclease Targeting Specific Transfer RNA Anticodons

T. Ogawa, 1 K. Tomita, 2* T. Ueda, 2 K. Watanabe, 2 T. Uozumi, 1 H. Masaki 1dagger

The carboxyl-terminal domain of colicin E5 was shown to inhibit protein synthesis of Escherichia coli. Its target, as revealed through in vivo and in vitro experiments, was not ribosomes as in the case of E3, but the transfer RNAs (tRNAs) for Tyr, His, Asn, and Asp, which contain a modified base, queuine, at the wobble position of each anticodon. The E5 carboxyl-terminal domain hydrolyzed these tRNAs just on the 3' side of this nucleotide. Tight correlation was observed between the toxicity of E5 and the cleavage of intracellular tRNAs of this group, implying that these tRNAs are the primary targets of colicin E5.

1 Department of Biotechnology, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan.
2 Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-8654, Japan.
*   Present address: Institut de Biologie Moléculaire des Plantes du CNRS, 12 rue du Général Zimmer, F-67084, Strasbourg Cedex, France.

dagger    To whom correspondence should be addressed. E-mail: hmasaki{at}mcb.bt.a.u-tokyo.ac.jp

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Reconstituting Bacterial RNA Repair and Modification in Vitro.
C. M. Chan, C. Zhou, and R. H. Huang (2009)
Science 326, 247
   Abstract »    Full Text »    PDF »
Structure-activity relationships in human RNA 3'-phosphate cyclase.
N. Tanaka and S. Shuman (2009)
RNA 15, 1865-1874
   Abstract »    Full Text »    PDF »
Structure-activity relationships in Kluyveromyces lactis {gamma}-toxin, a eukaryal tRNA anticodon nuclease.
N. Keppetipola, R. Jain, B. Meineke, M. Diver, and S. Shuman (2009)
RNA 15, 1036-1044
   Abstract »    Full Text »    PDF »
Colicin E5 Ribonuclease Domain Cleaves Saccharomyces cerevisiae tRNAs Leading to Impairment of the Cell Growth.
T. Ogawa, M. Hidaka, K. Kohno, and H. Masaki (2009)
J. Biochem. 145, 461-466
   Abstract »    Full Text »    PDF »
A Phosphate-binding Histidine of Binuclear Metallophosphodiesterase Enzymes Is a Determinant of 2',3'-Cyclic Nucleotide Phosphodiesterase Activity.
N. Keppetipola and S. Shuman (2008)
J. Biol. Chem. 283, 30942-30949
   Abstract »    Full Text »    PDF »
Stress-induced tRNA-derived RNAs: a novel class of small RNAs in the primitive eukaryote Giardia lamblia.
Y. Li, J. Luo, H. Zhou, J.-Y. Liao, L.-M. Ma, Y.-Q. Chen, and L.-H. Qu (2008)
Nucleic Acids Res. 36, 6048-6055
   Abstract »    Full Text »    PDF »
tRNA cleavage is a conserved response to oxidative stress in eukaryotes.
D. M. Thompson, C. Lu, P. J. Green, and R. Parker (2008)
RNA 14, 2095-2103
   Abstract »    Full Text »    PDF »
Kluyveromyces lactis {gamma}-toxin, a ribonuclease that recognizes the anticodon stem loop of tRNA.
J. Lu, A. Esberg, B. Huang, and A. S. Bystrom (2008)
Nucleic Acids Res. 36, 1072-1080
   Abstract »    Full Text »    PDF »
Developmentally regulated cleavage of tRNAs in the bacterium Streptomyces coelicolor.
H. J. Haiser, F. V. Karginov, G. J. Hannon, and M. A. Elliot (2008)
Nucleic Acids Res. 36, 732-741
   Abstract »    Full Text »    PDF »
Characterization of the 2',3' cyclic phosphodiesterase activities of Clostridium thermocellum polynucleotide kinase-phosphatase and bacteriophage {lambda} phosphatase.
N. Keppetipola and S. Shuman (2007)
Nucleic Acids Res. 35, 7721-7732
   Abstract »    Full Text »    PDF »
Reprogramming the tRNA-splicing activity of a bacterial RNA repair enzyme.
N. Keppetipola, J. Nandakumar, and S. Shuman (2007)
Nucleic Acids Res. 35, 3624-3630
   Abstract »    Full Text »    PDF »
Colicin Biology.
E. Cascales, S. K. Buchanan, D. Duche, C. Kleanthous, R. Lloubes, K. Postle, M. Riley, S. Slatin, and D. Cavard (2007)
Microbiol. Mol. Biol. Rev. 71, 158-229
   Abstract »    Full Text »    PDF »
Release of Immunity Protein Requires Functional Endonuclease Colicin Import Machinery.
D. Duche, A. Frenkian, V. Prima, and R. Lloubes (2006)
J. Bacteriol. 188, 8593-8600
   Abstract »    Full Text »    PDF »
Sequence-specific recognition of colicin E5, a tRNA-targeting ribonuclease.
T. Ogawa, S. Inoue, S. Yajima, M. Hidaka, and H. Masaki (2006)
Nucleic Acids Res. 34, 6065-6073
   Abstract »    Full Text »    PDF »
Structural basis for sequence-dependent recognition of colicin E5 tRNase by mimicking the mRNA-tRNA interaction.
S. Yajima, S. Inoue, T. Ogawa, T. Nonaka, K. Ohsawa, and H. Masaki (2006)
Nucleic Acids Res. 34, 6074-6082
   Abstract »    Full Text »    PDF »
Transfer RNA Cleavages by Onconase Reveal Unusual Cleavage Sites.
A. N. Suhasini and R. Sirdeshmukh (2006)
J. Biol. Chem. 281, 12201-12209
   Abstract »    Full Text »    PDF »
The Kluyveromyces lactis {gamma}-toxin targets tRNA anticodons.
J. LU, B. HUANG, A. ESBERG, M. J.O. JOHANSSON, and A. S. BYSTROM (2005)
RNA 11, 1648-1654
   Abstract »    Full Text »    PDF »
Rapid Detection of Colicin E9-Induced DNA Damage Using Escherichia coli Cells Carrying SOS Promoter-lux Fusions.
M. Vankemmelbeke, B. Healy, G. R. Moore, C. Kleanthous, C. N. Penfold, and R. James (2005)
J. Bacteriol. 187, 4900-4907
   Abstract »    Full Text »    PDF »
Flexibility in the Receptor-Binding Domain of the Enzymatic Colicin E9 Is Required for Toxicity against Escherichia coli Cells.
C. N. Penfold, B. Healy, N. G. Housden, R. Boetzel, M. Vankemmelbeke, G. R. Moore, C. Kleanthous, and R. James (2004)
J. Bacteriol. 186, 4520-4527
   Abstract »    Full Text »    PDF »
Mutagenic scan of the H-N-H motif of colicin E9: implications for the mechanistic enzymology of colicins, homing enzymes and apoptotic endonucleases.
D. C. Walker, T. Georgiou, A. J. Pommer, D. Walker, G. R. Moore, C. Kleanthous, and R. James (2002)
Nucleic Acids Res. 30, 3225-3234
   Abstract »    Full Text »    PDF »
Genetic Organization of Plasmid ColJs, Encoding Colicin Js Activity, Immunity, and Release Genes.
D. Smajs and G. M. Weinstock (2001)
J. Bacteriol. 183, 3949-3957
   Abstract »    Full Text »    PDF »
A cytotoxic ribonuclease which specifically cleaves four isoaccepting arginine tRNAs at their anticodon loops.
K. Tomita, T. Ogawa, T. Uozumi, K. Watanabe, and H. Masaki (2000)
PNAS
   Abstract »    Full Text »
Homing in on the Role of Transition Metals in the HNH Motif of Colicin Endonucleases.
A. J. Pommer, U. C. Kuhlmann, A. Cooper, A. M. Hemmings, G. R. Moore, R. James, and C. Kleanthous (1999)
J. Biol. Chem. 274, 27153-27160
   Abstract »    Full Text »    PDF »
Ribonuclease Activity of Rat Liver Perchloric Acid-soluble Protein, a Potent Inhibitor of Protein Synthesis.
R. Morishita, A. Kawagoshi, T. Sawasaki, K. Madin, T. Ogasawara, T. Oka, and Y. Endo (1999)
J. Biol. Chem. 274, 20688-20692
   Abstract »    Full Text »    PDF »
A cytotoxic ribonuclease which specifically cleaves four isoaccepting arginine tRNAs at their anticodon loops.
K. Tomita, T. Ogawa, T. Uozumi, K. Watanabe, and H. Masaki (2000)
PNAS 97, 8278-8283
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)