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Science 26 March 1999:
Vol. 283. no. 5410, pp. 2092 - 2095
DOI: 10.1126/science.283.5410.2092
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Reports

Role of the S. typhimurium Actin-Binding Protein SipA in Bacterial Internalization

Daoguo Zhou, 1 Mark S. Mooseker, 2 Jorge E. Galán 1*

Entry of the bacterium Salmonella typhimurium into host cells requires membrane ruffling and rearrangement of the actin cytoskeleton. Here, it is shown that the bacterial protein SipA plays a critical role in this process. SipA binds directly to actin, decreases its critical concentration, and inhibits depolymerization of actin filaments. These activities result in the spatial localization and more pronounced outward extension of the Salmonella-induced membrane ruffles, thereby facilitating bacterial uptake.

1 Section of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale School of Medicine, New Haven, CT 06536, USA.
2 Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA.
*   To whom correspondence should be addressed. E-mail: jorge.galan{at}yale.edu

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