Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 26 March 1999:
Vol. 283. no. 5410, pp. 2083 - 2085
DOI: 10.1126/science.283.5410.2083
Prev | Table of Contents | Next

Reports

Inhibition of Myosin Light Chain Kinase by p21-Activated Kinase

Luraynne C. Sanders, 1 Fumio Matsumura, 2 Gary M. Bokoch, 1* Primal de Lanerolle 3*

p21-activated kinases (PAKs) are implicated in the cytoskeletal changes induced by the Rho family of guanosine triphosphatases. Cytoskeletal dynamics are primarily modulated by interactions of actin and myosin II that are regulated by myosin light chain kinase (MLCK)-mediated phosphorylation of the regulatory myosin light chain (MLC). p21-activated kinase 1 (PAK1) phosphorylates MLCK, resulting in decreased MLCK activity. MLCK activity and MLC phosphorylation were decreased, and cell spreading was inhibited in baby hamster kidney-21 and HeLa cells expressing constitutively active PAK1. These data indicate that MLCK is a target for PAKs and that PAKs may regulate cytoskeletal dynamics by decreasing MLCK activity and MLC phosphorylation.

1 Departments of Immunology and Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.
2 Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, NJ 08855, USA.
3 Department of Physiology and Biophysics, University of Illinois at Chicago, Chicago, IL 60612, USA.
*   To whom correspondence should be addressed. E-mail: bokoch{at}scripps.edu; primal{at}uic.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Diacylglycerol Kinase {zeta} Regulates Actin Cytoskeleton Reorganization through Dissociation of Rac1 from RhoGDI.
H. Abramovici, P. Mojtabaie, R. J. Parks, X.-P. Zhong, G. A. Koretzky, M. K. Topham, and S. H. Gee (2009)
Mol. Biol. Cell 20, 2049-2059
   Abstract »    Full Text »    PDF »
Trihydrophobin 1 Interacts with PAK1 and Regulates ERK/MAPK Activation and Cell Migration.
C. Cheng, X. Kong, H. Wang, H. Gan, Y. Hao, W. Zou, J. Wu, Y. Chi, J. Yang, Y. Hong, et al. (2009)
J. Biol. Chem. 284, 8786-8796
   Abstract »    Full Text »    PDF »
Integrins in cell migration - the actin connection.
M. Vicente-Manzanares, C. K. Choi, and A. R. Horwitz (2009)
J. Cell Sci. 122, 199-206
   Abstract »    Full Text »    PDF »
Traction stress in focal adhesions correlates biphasically with actin retrograde flow speed.
M. L. Gardel, B. Sabass, L. Ji, G. Danuser, U. S. Schwarz, and C. M. Waterman (2008)
J. Cell Biol. 183, 999-1005
   Abstract »    Full Text »    PDF »
Schizosaccharomyces pombe Pak-related protein, Pak1p/Orb2p, phosphorylates myosin regulatory light chain to inhibit cytokinesis.
T.-H. Loo and M. Balasubramanian (2008)
J. Cell Biol. 183, 785-793
   Abstract »    Full Text »    PDF »
PAK1-mediated activation of ERK1/2 regulates lamellipodial dynamics.
S. D. Smith, Z. M. Jaffer, J. Chernoff, and A. J. Ridley (2008)
J. Cell Sci. 121, 3729-3736
   Abstract »    Full Text »    PDF »
Pak1 and Pak2 Mediate Tumor Cell Invasion through Distinct Signaling Mechanisms.
S. J. Coniglio, S. Zavarella, and M. H. Symons (2008)
Mol. Cell. Biol. 28, 4162-4172
   Abstract »    Full Text »    PDF »
16-kDa Prolactin Inhibits Endothelial Cell Migration by Down-Regulating the Ras-Tiam1-Rac1-Pak1 Signaling Pathway.
S.-H. Lee, J. Kunz, S.-H. Lin, and L.-y. Yu-Lee (2007)
Cancer Res. 67, 11045-11053
   Abstract »    Full Text »    PDF »
HGF attenuates thrombin-induced endothelial permeability by Tiam1-mediated activation of the Rac pathway and by Tiam1/Rac-dependent inhibition of the Rho pathway.
A. A. Birukova, E. Alekseeva, A. Mikaelyan, and K. G. Birukov (2007)
FASEB J 21, 2776-2786
   Abstract »    Full Text »    PDF »
p21-Activated kinase-1 and its role in integrated regulation of cardiac contractility.
K. A. Sheehan, Y. Ke, and R. J. Solaro (2007)
Am J Physiol Regulatory Integrative Comp Physiol 293, R963-R973
   Abstract »    Full Text »    PDF »
CTL-Associated Antigen-4 Ligation Induces Rapid T Cell Polarization That Depends on Phosphatidylinositol 3-Kinase, Vav-1, Cdc42, and Myosin Light Chain Kinase.
B. Wei, S. da Rocha Dias, H. Wang, and C. E. Rudd (2007)
J. Immunol. 179, 400-408
   Abstract »    Full Text »    PDF »
Increased Phosphorylation of Myosin Light Chain Prevents in Vitro Decidualization.
I. Ihnatovych, W. Hu, J. L. Martin, A. T. Fazleabas, P. de Lanerolle, and Z. Strakova (2007)
Endocrinology 148, 3176-3184
   Abstract »    Full Text »    PDF »
Induction of Vascular Permeability: betaPIX and GIT1 Scaffold the Activation of Extracellular Signal-regulated Kinase by PAK.
R. Stockton, J. Reutershan, D. Scott, J. Sanders, K. Ley, and M. A. Schwartz (2007)
Mol. Biol. Cell 18, 2346-2355
   Abstract »    Full Text »    PDF »
Role of myosin regulatory light chain and Rac1 in the migration of polyamine-depleted intestinal epithelial cells.
R. M. Ray, H. Guo, M. Patel, S. Jin, S. Bhattacharya, and L. R. Johnson (2007)
Am J Physiol Gastrointest Liver Physiol 292, G983-G995
   Abstract »    Full Text »    PDF »
Glucose-stimulated Cdc42 Signaling Is Essential for the Second Phase of Insulin Secretion.
Z. Wang, E. Oh, and D. C. Thurmond (2007)
J. Biol. Chem. 282, 9536-9546
   Abstract »    Full Text »    PDF »
Focal adhesion kinase modulates tension signaling to control actin and focal adhesion dynamics.
M. Schober, S. Raghavan, M. Nikolova, L. Polak, H. A. Pasolli, H. E. Beggs, L. F. Reichardt, and E. Fuchs (2007)
J. Cell Biol. 176, 667-680
   Abstract »    Full Text »    PDF »
Mechanism of polarized protrusion formation on neuronal precursors migrating in the developing chicken cerebellum.
A. Sakakibara and A. F. Horwitz (2006)
J. Cell Sci. 119, 3583-3592
   Abstract »    Full Text »    PDF »
Modulation of the Ca2+ sensitivity of airway smooth muscle cells in murine lung slices.
Y. Bai and M. J. Sanderson (2006)
Am J Physiol Lung Cell Mol Physiol 291, L208-L221
   Abstract »    Full Text »    PDF »
beta8 Integrin Binds Rho GDP Dissociation Inhibitor-1 and Activates Rac1 to Inhibit Mesangial Cell Myofibroblast Differentiation.
S. Lakhe-Reddy, S. Khan, M. Konieczkowski, G. Jarad, K. L. Wu, L. F. Reichardt, Y. Takai, L. A. Bruggeman, B. Wang, J. R. Sedor, et al. (2006)
J. Biol. Chem. 281, 19688-19699
   Abstract »    Full Text »    PDF »
Genetic Deletion of Rac1 GTPase Reveals Its Critical Role in Actin Stress Fiber Formation and Focal Adhesion Complex Assembly.
F. Guo, M. Debidda, L. Yang, D. A. Williams, and Y. Zheng (2006)
J. Biol. Chem. 281, 18652-18659
   Abstract »    Full Text »    PDF »
Paxillin phosphorylation at Ser273 localizes a GIT1-PIX-PAK complex and regulates adhesion and protrusion dynamics.
A. Nayal, D. J. Webb, C. M. Brown, E. M. Schaefer, M. Vicente-Manzanares, and A. R. Horwitz (2006)
J. Cell Biol. 173, 587-589
   Abstract »    Full Text »    PDF »
Novel Polymorphisms in the Myosin Light Chain Kinase Gene Confer Risk for Acute Lung Injury.
L. Gao, A. Grant, I. Halder, R. Brower, J. Sevransky, J. P. Maloney, M. Moss, C. Shanholtz, C. R. Yates, G. U. Meduri, et al. (2006)
Am. J. Respir. Cell Mol. Biol. 34, 487-495
   Abstract »    Full Text »    PDF »
Rac1 and RhoA Promote Neurite Outgrowth through Formation and Stabilization of Growth Cone Point Contacts.
S. Woo and T. M. Gomez (2006)
J. Neurosci. 26, 1418-1428
   Abstract »    Full Text »    PDF »
Ca2+-calmodulin-dependent myosin light chain kinase is essential for activation of TRPC5 channels expressed in HEK293 cells.
S. Shimizu, T. Yoshida, M. Wakamori, M. Ishii, T. Okada, M. Takahashi, M. Seto, K. Sakurada, Y. Kiuchi, and Y. Mori (2006)
J. Physiol. 570, 219-235
   Abstract »    Full Text »    PDF »
Signaling Mechanisms Regulating Endothelial Permeability.
D. Mehta and A. B. Malik (2006)
Physiol Rev 86, 279-367
   Abstract »    Full Text »    PDF »
PAK1 induces podosome formation in A7r5 vascular smooth muscle cells in a PAK-interacting exchange factor-dependent manner.
B. A. Webb, R. Eves, S. W. Crawley, S. Zhou, G. P. Cote, and A. S. Mak (2005)
Am J Physiol Cell Physiol 289, C898-C907
   Abstract »    Full Text »    PDF »
Myosin Phosphatase and Cofilin Mediate cAMP/cAMP-dependent Protein Kinase-induced Decline in Endothelial Cell Isometric Tension and Myosin II Regulatory Light Chain Phosphorylation.
Z. M. Goeckeler and R. B. Wysolmerski (2005)
J. Biol. Chem. 280, 33083-33095
   Abstract »    Full Text »    PDF »
Depolarization induces Rho-Rho kinase-mediated myosin light chain phosphorylation in kidney tubular cells.
K. Szaszi, G. Sirokmany, C. D. Ciano-Oliveira, O. D. Rotstein, and A. Kapus (2005)
Am J Physiol Cell Physiol 289, C673-C685
   Abstract »    Full Text »    PDF »
Inhibiting Myosin Light Chain Kinase Induces Apoptosis In Vitro and In Vivo.
F. Fazal, L. Gu, I. Ihnatovych, Y. Han, W. Hu, N. Antic, F. Carreira, J. F. Blomquist, T. J. Hope, D. S. Ucker, et al. (2005)
Mol. Cell. Biol. 25, 6259-6266
   Abstract »    Full Text »    PDF »
A microtubule-dependent zone of active RhoA during cleavage plane specification.
W. M. Bement, H. A. Benink, and G. von Dassow (2005)
J. Cell Biol. 170, 91-101
   Abstract »    Full Text »    PDF »
Rac2 Regulates Neutrophil Chemotaxis, Superoxide Production, and Myeloid Colony Formation through Multiple Distinct Effector Pathways.
D. Carstanjen, A. Yamauchi, A. Koornneef, H. Zang, M.-D. Filippi, C. Harris, J. Towe, S. Atkinson, Y. Zheng, M. C. Dinauer, et al. (2005)
J. Immunol. 174, 4613-4620
   Abstract »    Full Text »    PDF »
RSK4 and PAK5 Are Novel Candidate Genes in Diabetic Rat Kidney and Brain.
M. Niehof and J. Borlak (2005)
Mol. Pharmacol. 67, 604-611
   Abstract »    Full Text »    PDF »
Concentric zones of active RhoA and Cdc42 around single cell wounds.
H. A. Benink and W. M. Bement (2005)
J. Cell Biol. 168, 429-439
   Abstract »    Full Text »    PDF »
Adhesion Stimulates Direct PAK1/ERK2 Association and Leads to ERK-dependent PAK1 Thr212 Phosphorylation.
L. J. Sundberg-Smith, J. T. Doherty, C. P. Mack, and J. M. Taylor (2005)
J. Biol. Chem. 280, 2055-2064
   Abstract »    Full Text »    PDF »
The role of the Rho GTPases in neuronal development.
E.-E. Govek, S. E. Newey, and L. Van Aelst (2005)
Genes & Dev. 19, 1-49
   Abstract »    Full Text »    PDF »
The Small GTPase Cdc42 Regulates Actin Polymerization and Tension Development during Contractile Stimulation of Smooth Muscle.
D. D. Tang and S. J. Gunst (2004)
J. Biol. Chem. 279, 51722-51728
   Abstract »    Full Text »    PDF »
The coordination of signaling during Fc receptor-mediated phagocytosis.
J. A. Swanson and A. D. Hoppe (2004)
J. Leukoc. Biol. 76, 1093-1103
   Abstract »    Full Text »    PDF »
Protein Phosphatase 4 Is a Positive Regulator of Hematopoietic Progenitor Kinase 1.
G. Zhou, J. S. Boomer, and T.-H. Tan (2004)
J. Biol. Chem. 279, 49551-49561
   Abstract »    Full Text »    PDF »
p21-activated Kinase Regulates Endothelial Permeability through Modulation of Contractility.
R. A. Stockton, E. Schaefer, and M. A. Schwartz (2004)
J. Biol. Chem. 279, 46621-46630
   Abstract »    Full Text »    PDF »
Cell Type-specific Regulation of RhoA Activity during Cytokinesis.
H. Yoshizaki, Y. Ohba, M.-C. Parrini, N. G. Dulyaninova, A. R. Bresnick, N. Mochizuki, and M. Matsuda (2004)
J. Biol. Chem. 279, 44756-44762
   Abstract »    Full Text »    PDF »
Antagonistic Cross-talk between Rac and Cdc42 GTPases Regulates Generation of Reactive Oxygen Species.
B. A. Diebold, B. Fowler, J. Lu, M. C. Dinauer, and G. M. Bokoch (2004)
J. Biol. Chem. 279, 28136-28142
   Abstract »    Full Text »    PDF »
Urinary Bladder Contraction and Relaxation: Physiology and Pathophysiology.
K.-E. Andersson and A. Arner (2004)
Physiol Rev 84, 935-986
   Abstract »    Full Text »    PDF »
A Novel Role for p21-Activated Protein Kinase 2 in T Cell Activation.
P. C. Chu, J. Wu, X. C. Liao, J. Pardo, H. Zhao, C. Li, M. K. Mendenhall, E. Pali, M. Shen, S. Yu, et al. (2004)
J. Immunol. 172, 7324-7334
   Abstract »    Full Text »    PDF »
Disruption of the Actin Cytoskeleton Can Complement the Ability of Nef To Enhance Human Immunodeficiency Virus Type 1 Infectivity.
E. M. Campbell, R. Nunez, and T. J. Hope (2004)
J. Virol. 78, 5745-5755
   Abstract »    Full Text »    PDF »
A Point Mutation in the Motor Domain of Nonmuscle Myosin II-B Impairs Migration of Distinct Groups of Neurons.
X. Ma, S. Kawamoto, Y. Hara, and R. S. Adelstein (2004)
Mol. Biol. Cell 15, 2568-2579
   Abstract »    Full Text »    PDF »
Constitutive p21-activated Kinase (PAK) Activation in Breast Cancer Cells as a Result of Mislocalization of PAK to Focal Adhesions.
M. R. Stofega, L. C. Sanders, E. M. Gardiner, and G. M. Bokoch (2004)
Mol. Biol. Cell 15, 2965-2977
   Abstract »    Full Text »    PDF »
Involvement of Myosin II in Dopamine-induced Reorganization of the Lactotroph Cell's Actin Cytoskeleton.
M. L. Vitale and M. E. Carbajal (2004)
J. Histochem. Cytochem. 52, 517-527
   Abstract »    Full Text »    PDF »
CD29 integrin- and LIMK1/cofilin-mediated actin reorganization regulates the migration of haematopoietic progenitor cells underneath bone marrow stromal cells.
S. Konakahara, K. Ohashi, K. Mizuno, K. Itoh, and T. Tsuji (2004)
Genes Cells 9, 345-358
   Abstract »    Full Text »    PDF »
Mitogen-Activated Protein Kinase Feedback Phosphorylation Regulates MEK1 Complex Formation and Activation during Cellular Adhesion.
S. T. Eblen, J. K. Slack-Davis, A. Tarcsafalvi, J. T. Parsons, M. J. Weber, and A. D. Catling (2004)
Mol. Cell. Biol. 24, 2308-2317
   Abstract »    Full Text »    PDF »
Direct Interaction of p21-Activated Kinase 4 with PDZ-RhoGEF, a G Protein-linked Rho Guanine Exchange Factor.
A. Barac, J. Basile, J. Vazquez-Prado, Y. Gao, Y. Zheng, and J. S. Gutkind (2004)
J. Biol. Chem. 279, 6182-6189
   Abstract »    Full Text »    PDF »
Lessons From Old and New Kinases.
J.E. Van Eyk (2004)
Circ. Res. 94, 135-137
   Full Text »    PDF »
Distinct roles of MLCK and ROCK in the regulation of membrane protrusions and focal adhesion dynamics during cell migration of fibroblasts.
G. Totsukawa, Y. Wu, Y. Sasaki, D. J. Hartshorne, Y. Yamakita, S. Yamashiro, and F. Matsumura (2004)
J. Cell Biol. 164, 427-439
   Abstract »    Full Text »    PDF »
Pharmacogenomic Analysis of Cytogenetic Response in Chronic Myeloid Leukemia Patients Treated with Imatinib.
L. A. McLean, I. Gathmann, R. Capdeville, M. H. Polymeropoulos, and M. Dressman (2004)
Clin. Cancer Res. 10, 155-165
   Abstract »    Full Text »    PDF »
Rho-kinase-mediated Ca2+-independent contraction in rat embryo fibroblasts.
D. A. Emmert, J. A. Fee, Z. M. Goeckeler, J. M. Grojean, T. Wakatsuki, E. L. Elson, B. P. Herring, P. J. Gallagher, and R. B. Wysolmerski (2004)
Am J Physiol Cell Physiol 286, C8-C21
   Abstract »    Full Text »    PDF »
The Novel Rho GTPase-activating Protein Family Protein, Rga8, Provides a Potential Link between Cdc42/p21-activated Kinase and Rho Signaling Pathways in the Fission Yeast, Schizosaccharomyces pombe.
P. Yang, Y. Qyang, G. Bartholomeusz, X. Zhou, and S. Marcus (2003)
J. Biol. Chem. 278, 48821-48830
   Abstract »    Full Text »    PDF »
Akt Phosphorylation of Serine 21 on Pak1 Modulates Nck Binding and Cell Migration.
G.-L. Zhou, Y. Zhuo, C. C. King, B. H. Fryer, G. M. Bokoch, and J. Field (2003)
Mol. Cell. Biol. 23, 8058-8069
   Abstract »    Full Text »    PDF »
Myosin IIA Drives Neurite Retraction.
S. R. Wylie and P. D. Chantler (2003)
Mol. Biol. Cell 14, 4654-4666
   Abstract »    Full Text »    PDF »
Distinct Rho GTPase Activities Regulate Epithelial Cell Localization of the Adhesion Molecule CEACAM1: Involvement of the CEACAM1 Transmembrane Domain.
B. Fournes, J. Farrah, M. Olson, N. Lamarche-Vane, and N. Beauchemin (2003)
Mol. Cell. Biol. 23, 7291-7304
   Abstract »    Full Text »    PDF »
Ca2+ Sensitivity of Smooth Muscle and Nonmuscle Myosin II: Modulated by G Proteins, Kinases, and Myosin Phosphatase.
A. P. SOMLYO and A. V. SOMLYO (2003)
Physiol Rev 83, 1325-1358
   Abstract »    Full Text »    PDF »
LFA-1-induced T cell migration on ICAM-1 involves regulation of MLCK-mediated attachment and ROCK-dependent detachment.
A. Smith, M. Bracke, B. Leitinger, J. C. Porter, and N. Hogg (2003)
J. Cell Sci. 116, 3123-3133
   Abstract »    Full Text »    PDF »
Activity of Rho-family GTPases during cell division as visualized with FRET-based probes.
H. Yoshizaki, Y. Ohba, K. Kurokawa, R. E. Itoh, T. Nakamura, N. Mochizuki, K. Nagashima, and M. Matsuda (2003)
J. Cell Biol. 162, 223-232
   Abstract »    Full Text »    PDF »
PAK1 phosphorylation of MEK1 regulates fibronectin-stimulated MAPK activation.
J. K. Slack-Davis, S. T. Eblen, M. Zecevic, S. A. Boerner, A. Tarcsafalvi, H. B. Diaz, M. S. Marshall, M. J. Weber, J. T. Parsons, and A. D. Catling (2003)
J. Cell Biol. 162, 281-291
   Abstract »    Full Text »    PDF »
`Eph'ective signaling: forward, reverse and crosstalk.
K. K. Murai and E. B. Pasquale (2003)
J. Cell Sci. 116, 2823-2832
   Abstract »    Full Text »    PDF »
The Murine Nck SH2/SH3 Adaptors Are Important for the Development of Mesoderm-Derived Embryonic Structures and for Regulating the Cellular Actin Network.
F. Bladt, E. Aippersbach, S. Gelkop, G. A. Strasser, P. Nash, A. Tafuri, F. B. Gertler, and T. Pawson (2003)
Mol. Cell. Biol. 23, 4586-4597
   Abstract »    Full Text »    PDF »
Inhibition of contraction and myosin light chain phosphorylation in guinea-pig smooth muscle by p21-activated kinase 1.
A Wirth, M Schroeter, C Kock-Hauser, E Manser, J M Chalovich, P de Lanerolle, and G Pfitzer (2003)
J. Physiol. 549, 489-500
   Abstract »    Full Text »    PDF »
The C-terminal Kinase Domain of the p34cdc2-related PITSLRE Protein Kinase (p110C) Associates with p21-activated Kinase 1 and Inhibits Its Activity during Anoikis.
S. Chen, X. Yin, X. Zhu, J. Yan, S. Ji, C. Chen, M. Cai, S. Zhang, H. Zong, Y. Hu, et al. (2003)
J. Biol. Chem. 278, 20029-20036
   Abstract »    Full Text »    PDF »
The Prostate-derived Sterile 20-like Kinase (PSK) Regulates Microtubule Organization and Stability.
C. Mitsopoulos, C. Zihni, R. Garg, A. J. Ridley, and J. D. H. Morris (2003)
J. Biol. Chem. 278, 18085-18091
   Abstract »    Full Text »    PDF »
Modulation of Myosin Light-Chain Phosphorylation by p21-Activated Kinase 1 in Escherichia coli Invasion of Human Brain Microvascular Endothelial Cells.
R. S. Rudrabhatla, S. K. Sukumaran, G. M. Bokoch, and N. V. Prasadarao (2003)
Infect. Immun. 71, 2787-2797
   Abstract »    Full Text »    PDF »
Calcium-independent contraction and sensitization of airway smooth muscle by p21-activated protein kinase.
P. K. McFawn, L. Shen, S. G. Vincent, A. Mak, J. E. Van Eyk, and J. T. Fisher (2003)
Am J Physiol Lung Cell Mol Physiol 284, L863-L870
   Abstract »    Full Text »    PDF »
Mechanics of cell spreading: role of myosin II.
T. Wakatsuki, R. B. Wysolmerski, and E. L. Elson (2003)
J. Cell Sci. 116, 1617-1625
   Abstract »    Full Text »    PDF »
ERK and RhoA Differentially Regulate Pseudopodia Growth and Retraction during Chemotaxis.
A. A. Brahmbhatt and R. L. Klemke (2003)
J. Biol. Chem. 278, 13016-13025
   Abstract »    Full Text »    PDF »
EhPAK, a member of the p21-activated kinase family, is involved in the control of Entamoeba histolytica migration and phagocytosis.
E. Labruyere, C. Zimmer, V. Galy, J.-C. Olivo-Marin, and N. Guillen (2003)
J. Cell Sci. 116, 61-71
   Abstract »    Full Text »    PDF »
Cdc42/Rac1-dependent activation of the p21-activated kinase (PAK) regulates human platelet lamellipodia spreading: implication of the cortical-actin binding protein cortactin.
C. Vidal, B. Geny, J. Melle, M. Jandrot-Perrus, and M. Fontenay-Roupie (2002)
Blood 100, 4462-4469
   Abstract »    Full Text »    PDF »
Nogo-A and Myelin-Associated Glycoprotein Mediate Neurite Growth Inhibition by Antagonistic Regulation of RhoA and Rac1.
B. Niederost, T. Oertle, J. Fritsche, R. A. McKinney, and C. E. Bandtlow (2002)
J. Neurosci. 22, 10368-10376
   Abstract »    Full Text »    PDF »
Increased Spreading, Rac/p21-activated Kinase (PAK) Activity, and Compromised Cell Motility in Cells Deficient in Vasodilator-stimulated Phosphoprotein (VASP).
M. I. Garcia Arguinzonis, A. B. Galler, U. Walter, M. Reinhard, and A. Simm (2002)
J. Biol. Chem. 277, 45604-45610
   Abstract »    Full Text »    PDF »
Rac2, a Hematopoiesis-Specific Rho GTPase, Specifically Regulates Mast Cell Protease Gene Expression in Bone Marrow-Derived Mast Cells.
Y. Gu, M. C. Byrne, N. C. Paranavitana, B. Aronow, J. E. Siefring, M. D'Souza, H. F. Horton, L. A. Quilliam, and D. A. Williams (2002)
Mol. Cell. Biol. 22, 7645-7657
   Abstract »    Full Text »    PDF »
CDK5 Regulates Cell Adhesion and Migration in Corneal Epithelial Cells.
C. Gao, S. Negash, H. T. Guo, D. Ledee, H.-S. Wang, and P. Zelenka (2002)
Mol. Cancer Res. 1, 12-24
   Abstract »    Full Text »    PDF »
Single Cell Behavior in Metastatic Primary Mammary Tumors Correlated with Gene Expression Patterns Revealed by Molecular Profiling.
W. Wang, J. B. Wyckoff, V. C. Frohlich, Y. Oleynikov, S. Huttelmaier, J. Zavadil, L. Cermak, E. P. Bottinger, R. H. Singer, J. G. White, et al. (2002)
Cancer Res. 62, 6278-6288
   Abstract »    Full Text »    PDF »
p21-activated kinase 4 interacts with integrin {alpha}v{beta}5 and regulates {alpha}v{beta}5-mediated cell migration.
H. Zhang, Z. Li, E.-K. Viklund, and S. Stromblad (2002)
J. Cell Biol. 158, 1287-1297
   Abstract »    Full Text »    PDF »
Association of PI-3 Kinase with PAK1 Leads to Actin Phosphorylation and Cytoskeletal Reorganization.
E. A. Papakonstanti and C. Stournaras (2002)
Mol. Biol. Cell 13, 2946-2962
   Abstract »    Full Text »    PDF »
Microtubule-Actin Cross-talk at Focal Adhesions.
A. F. Palazzo and G. G. Gundersen (2002)
Sci. STKE 2002, pe31
   Abstract »    Full Text »    PDF »
Differential regulation of Rho GTPases by {beta}1 and {beta}3 integrins: the role of an extracellular domain of integrin in intracellular signaling.
H. Miao, S. Li, Y.-L. Hu, S. Yuan, Y. Zhao, B. P. C. Chen, W. Puzon-McLaughlin, T. Tarui, J. Y.-J. Shyy, Y. Takada, et al. (2002)
J. Cell Sci. 115, 2199-2206
   Abstract »    Full Text »    PDF »
A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows.
T.-L. Chew, W. A. Wolf, P. J. Gallagher, F. Matsumura, and R. L. Chisholm (2002)
J. Cell Biol. 156, 543-553
   Abstract »    Full Text »    PDF »
Rho GTPases link cytoskeletal rearrangements and activation processes induced via the tetraspanin CD82 in T lymphocytes.
A. Delaguillaumie, C. Lagaudriere-Gesbert, M. R. Popoff, and H. Conjeaud (2002)
J. Cell Sci. 115, 433-443
   Abstract »    Full Text »    PDF »
GIT1 functions in a motile, multi-molecular signaling complex that regulates protrusive activity and cell migration.
R.-i. Manabe, M. Kovalenko, D. J. Webb, and A. R. Horwitz (2002)
J. Cell Sci. 115, 1497-1510
   Abstract »    Full Text »    PDF »
Cytoskeletal regulation of pulmonary vascular permeability.
S. M. Dudek and J. G. N. Garcia (2001)
J Appl Physiol 91, 1487-1500
   Abstract »    Full Text »    PDF »
Hic-5-Reduced Cell Spreading on Fibronectin: Competitive Effects between Paxillin and Hic-5 through Interaction with Focal Adhesion Kinase.
N. Nishiya, K. Tachibana, M. Shibanuma, J.-i. Mashimo, and K. Nose (2001)
Mol. Cell. Biol. 21, 5332-5345
   Abstract »    Full Text »    PDF »
Signal Transduction in Smooth Muscle: Historical perspective on airway smooth muscle: the saga of a frustrated cell.
C. Y. Seow and J. J. Fredberg (2001)
J Appl Physiol 91, 938-952
   Abstract »    Full Text »    PDF »
Signal Transduction in Smooth Muscle: Invited Review: Regulation of myosin phosphorylation in smooth muscle.
G. Pfitzer (2001)
J Appl Physiol 91, 497-503
   Abstract »    Full Text »    PDF »
p21-activated kinase 1 participates in tracheal smooth muscle cell migration by signaling to p38 MAPK.
M. A. Dechert, J. M. Holder, and W. T. Gerthoffer (2001)
Am J Physiol Cell Physiol 281, C123-C132
   Abstract »    Full Text »    PDF »
Regulation by GDI of RhoA/Rho-kinase-induced Ca2+ sensitization of smooth muscle myosin II.
M. C. Gong, I. Gorenne, P. Read, T. Jia, R. K. Nakamoto, A. V. Somlyo, and A. P. Somlyo (2001)
Am J Physiol Cell Physiol 281, C257-C269
   Abstract »    Full Text »    PDF »
Activation of RhoA and ROCK Are Essential for Detachment of Migrating Leukocytes.
J. Alblas, L. Ulfman, P. Hordijk, and L. Koenderman (2001)
Mol. Biol. Cell 12, 2137-2145
   Abstract »    Full Text »    PDF »
Regulation of the p21-activated kinase (PAK) by a human Gbeta -like WD-repeat protein, hPIP1.
C. Xia, W. Ma, L. J. Stafford, S. Marcus, W.-C. Xiong, and M. Liu (2001)
PNAS 98, 6174-6179
   Abstract »    Full Text »    PDF »
Activated PAK4 Regulates Cell Adhesion and Anchorage-Independent Growth.
J. Qu, M. S. Cammarano, Q. Shi, K. C. Ha, P. de Lanerolle, and A. Minden (2001)
Mol. Cell. Biol. 21, 3523-3533
   Abstract »    Full Text »
Cofilin Phosphorylation by Protein Kinase Testicular Protein Kinase 1 and Its Role in Integrin-mediated Actin Reorganization and Focal Adhesion Formation.
J. Toshima, J. Y. Toshima, T. Amano, N. Yang, S. Narumiya, and K. Mizuno (2001)
Mol. Biol. Cell 12, 1131-1145
   Abstract »    Full Text »
Cross Talk Between Cyclic Nucleotides and Polyphosphoinositide Hydrolysis, Protein Kinases, and Contraction in Smooth Muscle.
A. A. Abdel-Latif (2001)
Experimental Biology and Medicine 226, 153-163
   Abstract »    Full Text »
Cytoskeletal Effects of Rho-Like Small Guanine Nucleotide-Binding Proteins in the Vascular System.
G. P. van Nieuw Amerongen and V. W.M. van Hinsbergh (2001)
Arterioscler. Thromb. Vasc. Biol. 21, 300-311
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)