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Science 12 March 1999:
Vol. 283. no. 5408, pp. 1739 - 1742
DOI: 10.1126/science.283.5408.1739
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Reports

Negative Regulation of Wingless Signaling by D-Axin, a Drosophila Homolog of Axin

Fumihiko Hamada, 12 Yoshinori Tomoyasu, 3 Yoshihiro Takatsu, 3 Makoto Nakamura, 3 Shin-ichi Nagai, 3 Akiko Suzuki, 1 Fumitaka Fujita, 1 Hiroshi Shibuya, 3 Kumao Toyoshima, 4 Naoto Ueno, 3 Tetsu Akiyama 15*

Wnt/Wingless directs many cell fates during development. Wnt/Wingless signaling increases the amount of beta -catenin/Armadillo, which in turn activates gene transcription. Here the Drosophila protein D-Axin was shown to interact with Armadillo and D-APC. Mutation of d-axin resulted in the accumulation of cytoplasmic Armadillo and one of the Wingless target gene products, Distal-less. Ectopic expression of d-axin inhibited Wingless signaling. Hence, D-Axin negatively regulates Wingless signaling by down-regulating the level of Armadillo. These results establish the importance of the Axin family of proteins in Wnt/Wingless signaling in Drosophila.

1 Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, Japan.
2 "Inheritance and Variation," Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Corporation (JST), Japan.
3 Division of Morphogenesis, Department of Developmental Biology, National Institute for Basic Biology, Myodaijicho, Okazaki 444-8585, Japan.
4 Osaka Medical Center for Cancer and Cardiovascular Diseases, Nakamichi, Higashinari-ku, Osaka 537-8511, Japan.
5 Department of Molecular and Genetic Information, Institute of Molecular and Cellular Biosciences, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan.
*   To whom correspondence should be addressed. E-mail: akiyama{at}imcbns.iam.u-tokyo.ac.jp

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