Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 12 March 1999:
Vol. 283. no. 5408, pp. 1736 - 1739
DOI: 10.1126/science.283.5408.1736
Prev | Table of Contents | Next

Reports

Role of Pitx1 Upstream of Tbx4 in Specification of Hindlimb Identity

Malcolm Logan, Clifford J. Tabin *

In spite of recent breakthroughs in understanding limb patterning, the genetic factors determining the differences between the forelimb and the hindlimb have not been understood. The genes Pitx1 and Tbx4 encode transcription factors that are expressed throughout the developing hindlimb but not forelimb buds. Misexpression of Pitx1 in the chick wing bud induced distal expression of Tbx4, as well as HoxC10 and HoxC11, which are normally restricted to hindlimb expression domains. Wing buds in which Pitx1 was misexpressed developed into limbs with some morphological characteristics of hindlimbs: the flexure was altered to that normally observed in legs, the digits were more toe-like in their relative size and shape, and the muscle pattern was transformed to that of a leg.

Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
*   To whom correspondence should be addressed. E-mail: tabin{at}rascal.med.harvard.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Dual hindlimb control elements in the Tbx4 gene and region-specific control of bone size in vertebrate limbs.
D. B. Menke, C. Guenther, and D. M. Kingsley (2008)
Development 135, 2543-2553
   Abstract »    Full Text »    PDF »
DUX4, a candidate gene of facioscapulohumeral muscular dystrophy, encodes a transcriptional activator of PITX1.
M. Dixit, E. Ansseau, A. Tassin, S. Winokur, R. Shi, H. Qian, S. Sauvage, C. Matteotti, A. M. van Acker, O. Leo, et al. (2007)
PNAS 104, 18157-18162
   Abstract »    Full Text »    PDF »
A Muscle-specific Promoter Directs Pitx3 Gene Expression in Skeletal Muscle Cells.
V. Coulon, A. L'Honore, J.-F. Ouimette, E. Dumontier, P. van den Munckhof, and J. Drouin (2007)
J. Biol. Chem. 282, 33192-33200
   Abstract »    Full Text »    PDF »
Tbx4 is not required for hindlimb identity or post-bud hindlimb outgrowth.
L. A. Naiche and V. E. Papaioannou (2007)
Development 134, 93-103
   Abstract »    Full Text »    PDF »
Parallel genetic origins of pelvic reduction in vertebrates.
M. D. Shapiro, M. A. Bell, and D. M. Kingsley (2006)
PNAS 103, 13753-13758
   Abstract »    Full Text »    PDF »
Isl1Cre reveals a common Bmp pathway in heart and limb development..
L. Yang, C.-L. Cai, L. Lin, Y. Qyang, C. Chung, R. M. Monteiro, C. L. Mummery, G. I. Fishman, A. Cogen, and S. Evans (2006)
Development 133, 1575-1585
   Abstract »    Full Text »    PDF »
Cell-specific Activation of the Atrial Natriuretic Factor Promoter by PITX2 and MEF2A.
R. Toro, I. Saadi, A. Kuburas, M. Nemer, and A. F. Russo (2004)
J. Biol. Chem. 279, 52087-52094
   Abstract »    Full Text »    PDF »
Finger or toe: the molecular basis of limb identity.
M. Logan (2003)
Development 130, 6401-6410
   Abstract »    Full Text »    PDF »
Analysis of a key regulatory region upstream of the Myf5 gene reveals multiple phases of myogenesis, orchestrated at each site by a combination of elements dispersed throughout the locus.
J. Hadchouel, J. J. Carvajal, P. Daubas, L. Bajard, T. Chang, D. Rocancourt, D. Cox, D. Summerbell, S. Tajbakhsh, P. W. J. Rigby, et al. (2003)
Development 130, 3415-3426
   Abstract »    Full Text »    PDF »
Loss of Tbx4 blocks hindlimb development and affects vascularization and fusion of the allantois.
L. A. Naiche and V. E. Papaioannou (2003)
Development 130, 2681-2693
   Abstract »    Full Text »    PDF »
Tbx5 is required for forelimb bud formation and continued outgrowth.
C. Rallis, B. G. Bruneau, J. Del Buono, C. E. Seidman, J. G. Seidman, S. Nissim, C. J. Tabin, and M. P. O. Logan (2003)
Development 130, 2741-2751
   Abstract »    Full Text »    PDF »
T-box genes in human disorders.
E. A. Packham and J. D. Brook (2003)
Hum. Mol. Genet. 12, R37-44
   Abstract »    Full Text »    PDF »
The limb identity gene Tbx5 promotes limb initiation by interacting with Wnt2b and Fgf10.
J. K. Ng, Y. Kawakami, D. Buscher, A. Raya, T. Itoh, C. M. Koth, C. R. Esteban, J. Rodriguez-Leon, D. M. Garrity, M. C. Fishman, et al. (2003)
Development 129, 5161-5170
   Abstract »    Full Text »    PDF »
Tbx5 is essential for forelimb bud initiation following patterning of the limb field in the mouse embryo.
P. Agarwal, J. N. Wylie, J. Galceran, O. Arkhitko, C. Li, C. Deng, R. Grosschedl, and B. G. Bruneau (2003)
Development 130, 623-633
   Abstract »    Full Text »    PDF »
Pitx1 and Pitx2 are required for development of hindlimb buds.
A. Marcil, E. Dumontier, M. Chamberland, S. A. Camper, and J. Drouin (2003)
Development 130, 45-55
   Abstract »    Full Text »    PDF »
Pitx Factors Are Involved in Basal and Hormone-regulated Activity of the Human Prolactin Promoter.
M.-H. Quentien, I. Manfroid, D. Moncet, G. Gunz, M. Muller, M. Grino, A. Enjalbert, and I. Pellegrini (2002)
J. Biol. Chem. 277, 44408-44416
   Abstract »    Full Text »    PDF »
The heartstrings mutation in zebrafish causes heart/fin Tbx5 deficiency syndrome.
D. M. Garrity, S. Childs, and M. C. Fishman (2002)
Development 129, 4635-4645
   Abstract »    Full Text »    PDF »
Determinants of T box protein specificity.
F. L. Conlon, L. Fairclough, B. M. J. Price, E. S. Casey, and J. C. Smith (2001)
Development 128, 3749-3758
   Abstract »    Full Text »    PDF »
A Single Pitx1 Binding Site Is Essential for Activity of the LH{beta} Promoter in Transgenic Mice.
C. C. Quirk, K. L. Lozada, R. A. Keri, and J. H. Nilson (2001)
Mol. Endocrinol. 15, 734-746
   Abstract »    Full Text »
Regulation of Mandibular Growth and Morphogenesis.
M. Mina (2001)
Critical Reviews in Oral Biology & Medicine 12, 276-300
   Abstract »    Full Text »    PDF »
Specific Protein-Protein Interaction between Basic Helix-Loop-Helix Transcription Factors and Homeoproteins of the Pitx Family.
G. Poulin, M. Lebel, M. Chamberland, F. W. Paradis, and J. Drouin (2000)
Mol. Cell. Biol. 20, 4826-4837
   Abstract »    Full Text »
Special Feature: Regulatory evolution and the origin of the bilaterians.
K. J. Peterson and E. H. Davidson (2000)
PNAS 97, 4430-4433
   Abstract »    Full Text »    PDF »
Genetic and developmental bases of serial homology in vertebrate limb evolution.
I Ruvinsky and J. Gibson-Brown (2000)
Development 127, 5233-5244
   Abstract »    PDF »
A Comparative Molecular Analysis of Developing Mouse Forelimbs and Hindlimbs Using Serial Analysis of Gene Expression (SAGE).
E. H. Margulies, S. L.R. Kardia, and J. W. Innis (2001)
Genome Res. 11, 1686-1698
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)