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Science 12 March 1999:
Vol. 283. no. 5408, pp. 1733 - 1735
DOI: 10.1126/science.283.5408.1733
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Reports

Abnormal Spindle Protein, Asp, and the Integrity of Mitotic Centrosomal Microtubule Organizing Centers

Maria do Carmo Avides, David M. Glover *

The product of the abnormal spindle (asp) gene was found to be an asymmetrically localized component of the centrosome during mitosis, required to focus the poles of the mitotic spindle in vivo. Removing Asp protein function from Drosophila melanogaster embryo extracts, either by mutation or immunodepletion, resulted in loss of their ability to restore microtubule-organizing center activity to salt-stripped centrosome preparations. This was corrected by addition of purified Asp protein. Thus, Asp appears to hold together the microtubule-nucleating gamma -tubulin ring complexes that organize the mitotic centrosome.

Cancer Research Campaign Cell Cycle Genetics Laboratories, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, UK.
*   To whom correspondence should be addressed. E-mail: d.m.glover{at}dundee.ac.uk

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