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Science 5 March 1999:
Vol. 283. no. 5407, pp. 1535 - 1538
DOI: 10.1126/science.283.5407.1535
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Reports

Adhesive and Mammalian Transglutaminase Substrate Properties of Candida albicans Hwp1

Janet F. Staab, 1 Steven D. Bradway, 2* Paul L. Fidel, 3 Paula Sundstrom 1dagger

The pathogenesis of candidiasis involves invasion of host tissues by filamentous forms of the opportunistic yeast Candida albicans. Morphology-specific gene products may confer proinvasive properties. A hypha-specific surface protein, Hwp1, with similarities to mammalian small proline-rich proteins was shown to serve as a substrate for mammalian transglutaminases. Candida albicans strains lacking Hwp1 were unable to form stable attachments to human buccal epithelial cells and had a reduced capacity to cause systemic candidiasis in mice. This represents a paradigm for microbial adhesion that implicates essential host enzymes.

1 Department of Medical Microbiology and Immunology, College of Medicine and Public Health,
2 Department of Periodontology, College of Dentistry, Ohio State University, 333 West Tenth Avenue, Columbus, OH 43210, USA.
3 Department of Microbiology, Immunology, and Parasitology, Louisiana State University Medical Center, 1901 Perdido Street, New Orleans, LA 70112, USA.
*   Present address: Grays Harbor Dental Specialists, 105 South Broadway, Aberdeen, WA 98520, USA.

dagger    To whom correspondence should be addressed. E-mail:sundstrom.1{at}osu.edu

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