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Science 19 February 1999:
Vol. 283. no. 5405, pp. 1161 - 1164
DOI: 10.1126/science.283.5405.1161
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Reports

Regulation of Chamber-Specific Gene Expression in the Developing Heart by Irx4

Zheng-Zheng Bao, 1 Benoit G. Bruneau, 1 J. G. Seidman, 1 Christine E. Seidman, 2 Constance L. Cepko 1*

The vertebrate heart consists of two types of chambers, the atria and the ventricles, which differ in their contractile and electrophysiological properties. Little is known of the molecular mechanisms by which these chambers are specified during embryogenesis. Here a chicken iroquois-related homeobox gene, Irx4, was identified that has a ventricle-restricted expression pattern at all stages of heart development. Irx4 protein was shown to regulate the chamber-specific expression of myosin isoforms by activating the expression of the ventricle myosin heavy chain-1 (VMHC1) and suppressing the expression of the atrial myosin heavy chain-1 (AMHC1) in the ventricles. Thus, Irx4 may play a critical role in establishing chamber-specific gene expression in the developing heart.

1 Howard Hughes Medical Institute and Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
2 Howard Hughes Medical Institute and Cardiovascular Division, Brigham and Women's Hospital, Boston, MA 02115, USA.
*   To whom correspondence should be addressed. E-mail: cepko{at}rascal.med.harvard.edu

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