Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 19 February 1999:
Vol. 283. no. 5405, pp. 1152 - 1157
DOI: 10.1126/science.283.5405.1152
Prev | Table of Contents | Next

Reports

A Processive Single-Headed Motor: Kinesin Superfamily Protein KIF1A

Yasushi Okada, Nobutaka Hirokawa *

A single kinesin molecule can move "processively" along a microtubule for more than 1 micrometer before detaching from it. The prevailing explanation for this processive movement is the "walking model," which envisions that each of two motor domains (heads) of the kinesin molecule binds coordinately to the microtubule. This implies that each kinesin molecule must have two heads to "walk" and that a single-headed kinesin could not move processively. Here, a motor-domain construct of KIF1A, a single-headed kinesin superfamily protein, was shown to move processively along the microtubule for more than 1 micrometer. The movement along the microtubules was stochastic and fitted a biased Brownian-movement model.

Department of Cell Biology and Anatomy, Graduate School of Medicine, University of Tokyo, Hongo, Tokyo 113-0033, Japan.
*   To whom correspondence should be addressed. E-mail: hirokawa{at}m.u-tokyo.ac.jp

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Aspergillus nidulans Kinesin-3 UncA Motor Moves Vesicles along a Subpopulation of Microtubules.
N. Zekert and R. Fischer (2009)
Mol. Biol. Cell 20, 673-684
   Abstract »    Full Text »    PDF »
Diffusion and Directed Movement: IN VITRO MOTILE PROPERTIES OF FISSION YEAST KINESIN-14 Pkl1.
K. Furuta, M. Edamatsu, Y. Maeda, and Y. Y. Toyoshima (2008)
J. Biol. Chem. 283, 36465-36473
   Abstract »    Full Text »    PDF »
CENP-E combines a slow, processive motor and a flexible coiled coil to produce an essential motile kinetochore tether.
Y. Kim, J. E. Heuser, C. M. Waterman, and D. W. Cleveland (2008)
J. Cell Biol. 181, 411-419
   Abstract »    Full Text »    PDF »
Intracellular Transport and Kinesin Superfamily Proteins, KIFs: Structure, Function, and Dynamics.
N. Hirokawa and Y. Noda (2008)
Physiol Rev 88, 1089-1118
   Abstract »    Full Text »    PDF »
Identification of an Axonal Kinesin-3 Motor for Fast Anterograde Vesicle Transport that Facilitates Retrograde Transport of Neuropeptides.
R. V. Barkus, O. Klyachko, D. Horiuchi, B. J. Dickson, and W. M. Saxton (2008)
Mol. Biol. Cell 19, 274-283
   Abstract »    Full Text »    PDF »
Loss of {alpha}-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function.
K. Ikegami, R. L. Heier, M. Taruishi, H. Takagi, M. Mukai, S. Shimma, S. Taira, K. Hatanaka, N. Morone, I. Yao, et al. (2007)
PNAS 104, 3213-3218
   Abstract »    Full Text »    PDF »
Kinetic and Mechanistic Basis of the Nonprocessive Kinesin-3 Motor NcKin3.
S. Adio, M. Bloemink, M. Hartel, S. Leier, M. A. Geeves, and G. Woehlke (2006)
J. Biol. Chem. 281, 37782-37793
   Abstract »    Full Text »    PDF »
Engineering cooperativity in biomotor-protein assemblies..
M. R. Diehl, K. Zhang, H. J. Lee, and D. A. Tirrell (2006)
Science 311, 1468-1471
   Abstract »    Full Text »    PDF »
An Axonemal Dynein Particularly Important for Flagellar Movement at High Viscosity: IMPLICATIONS FROM A NEW CHLAMYDOMONAS MUTANT DEFICIENT IN THE DYNEIN HEAVY CHAIN GENE DHC9.
T. Yagi, I. Minoura, A. Fujiwara, R. Saito, T. Yasunaga, M. Hirono, and R. Kamiya (2005)
J. Biol. Chem. 280, 41412-41420
   Abstract »    Full Text »    PDF »
Long-range cooperative binding of kinesin to a microtubule in the presence of ATP.
E. Muto, H. Sakai, and K. Kaseda (2005)
J. Cell Biol. 168, 691-696
   Abstract »    Full Text »    PDF »
Subdomain organization of the Acanthamoeba myosin IC tail from cryo-electron microscopy.
T. Ishikawa, N. Cheng, X. Liu, E. D. Korn, and A. C. Steven (2004)
PNAS 101, 12189-12194
   Abstract »    Full Text »    PDF »
The Lipid Binding Pleckstrin Homology Domain in UNC-104 Kinesin is Necessary for Synaptic Vesicle Transport in Caenorhabditis elegans.
D. R. Klopfenstein and R. D. Vale (2004)
Mol. Biol. Cell 15, 3729-3739
   Abstract »    Full Text »    PDF »
KIF1A Alternately Uses Two Loops to Bind Microtubules.
R. Nitta, M. Kikkawa, Y. Okada, and N. Hirokawa (2004)
Science 305, 678-683
   Abstract »    Full Text »    PDF »
A one-headed class V myosin molecule develops multiple large ({approx}32-nm) steps successively.
T. M. Watanabe, H. Tanaka, A. H. Iwane, S. Maki-Yonekura, K. Homma, A. Inoue, R. Ikebe, T. Yanagida, and M. Ikebe (2004)
PNAS 101, 9630-9635
   Abstract »    Full Text »    PDF »
Inhibition of kinesin motility by ADP and phosphate supports a hand-over-hand mechanism.
W. R. Schief, R. H. Clark, A. H. Crevenna, and J. Howard (2004)
PNAS 101, 1183-1188
   Abstract »    Full Text »    PDF »
The Kinesin Family Member BimC Contains a Second Microtubule Binding Region Attached to the N terminus of the Motor Domain.
M. F. Stock, J. Chu, and D. D. Hackney (2003)
J. Biol. Chem. 278, 52315-52322
   Abstract »    Full Text »    PDF »
Distinct conformations of the kinesin Unc104 neck regulate a monomer to dimer motor transition.
J. Al-Bassam, Y. Cui, D. Klopfenstein, B. O. Carragher, R. D. Vale, and R. A. Milligan (2003)
J. Cell Biol. 163, 743-753
   Abstract »    Full Text »    PDF »
The Number of Repeat Sequences in Microtubule-associated Protein 4 Affects the Microtubule Surface Properties.
K. Tokuraku, K. Matsushima, T. Matui, H. Nakagawa, M. Katsuki, R. Majima, and S. Kotani (2003)
J. Biol. Chem. 278, 29609-29618
   Abstract »    Full Text »    PDF »
The Second Microtubule-binding Site of Monomeric Kid Enhances the Microtubule Affinity.
K. Shiroguchi, M. Ohsugi, M. Edamatsu, T. Yamamoto, and Y. Y. Toyoshima (2003)
J. Biol. Chem. 278, 22460-22465
   Abstract »    Full Text »    PDF »
Association of the Kinesin Motor KIF1A with the Multimodular Protein Liprin-alpha.
H. Shin, M. Wyszynski, K.-H. Huh, J. G. Valtschanoff, J.-R. Lee, J. Ko, M. Streuli, R. J. Weinberg, M. Sheng, and E. Kim (2003)
J. Biol. Chem. 278, 11393-11401
   Abstract »    Full Text »    PDF »
Characterization of the Movement of the Kinesin Motor KIF1A in Living Cultured Neurons.
J.-R. Lee, H. Shin, J. Ko, J. Choi, H. Lee, and E. Kim (2003)
J. Biol. Chem. 278, 2624-2629
   Abstract »    Full Text »    PDF »
Conversion of Unc104/KIF1A Kinesin into a Processive Motor After Dimerization.
M. Tomishige, D. R. Klopfenstein, and R. D. Vale (2002)
Science 297, 2263-2267
   Abstract »    Full Text »    PDF »
Push or pull? Teams of motor proteins have it both ways.
T. Duke (2002)
PNAS 99, 6521-6523
   Full Text »    PDF »
Distinguishing Inchworm and Hand-Over-Hand Processive Kinesin Movement by Neck Rotation Measurements.
W. Hua, J. Chung, and J. Gelles (2002)
Science 295, 844-848
   Abstract »    Full Text »    PDF »
Kinesin: switch I & II and the motor mechanism.
F. J. Kull and S. A. Endow (2002)
J. Cell Sci. 115, 15-23
   Abstract »    Full Text »    PDF »
KIFC3, a microtubule minus end-directed motor for the apical transport of annexin XIIIb-associated Triton-insoluble membranes.
Y. Noda, Y. Okada, N. Saito, M. Setou, Y. Xu, Z. Zhang, and N. Hirokawa (2001)
J. Cell Biol. 155, 77-88
   Abstract »    Full Text »    PDF »
Direct Visualization of the Movement of the Monomeric Axonal Transport Motor UNC-104 along Neuronal Processes in Living Caenorhabditis elegans.
H. M. Zhou, I. Brust-Mascher, and J. M. Scholey (2001)
J. Neurosci. 21, 3749-3755
   Abstract »    Full Text »    PDF »
Myosin learns to walk.
A. Mehta (2001)
J. Cell Sci. 114, 1981-1998
   Abstract »    Full Text »    PDF »
Kinesin Processivity.
E. W. Taylor and G. G. Borisy (2000)
J. Cell Biol. 151, 27-30
   Full Text »    PDF »
Controlling Kinesin by Reversible Disulfide Cross-Linking: Identifying the Motility-Producing Conformational Change.
M. Tomishige and R. D. Vale (2000)
J. Cell Biol. 151, 1081-1092
   Abstract »    Full Text »    PDF »
The Kar3p Kinesin-related Protein Forms a Novel Heterodimeric Structure with Its Associated Protein Cik1p.
J. G. Barrett, B. D. Manning, and M. Snyder (2000)
Mol. Biol. Cell 11, 2373-2385
   Abstract »    Full Text »
The Way Things Move: Looking Under the Hood of Molecular Motor Proteins.
R. D. Vale and R. A. Milligan (2000)
Science 288, 88-95
   Abstract »    Full Text »
Functional Elements within the Dynein Microtubule-binding Domain.
M. P. Koonce and I. Tikhonenko (2000)
Mol. Biol. Cell 11, 523-529
   Abstract »    Full Text »
Mechanism of the single-headed processivity: Diffusional anchoring between the K-loop of kinesin and the C terminus of tubulin.
Y. Okada and N. Hirokawa (2000)
PNAS 97, 640-645
   Abstract »    Full Text »    PDF »
How motor proteins influence microtubule polymerization dynamics.
A. Hunter and L Wordeman (2000)
J. Cell Sci. 113, 4379-4389
   Abstract »    PDF »
Evidence for a Novel Affinity Mechanism of Motor-assisted Transport Along Microtubules.
Y. Wada, T. Hamasaki, and P. Satir (2000)
Mol. Biol. Cell 11, 161-169
   Abstract »    Full Text »
Kinesin's processivity results from mechanical and chemical coordination between the ATP hydrolysis cycles of the two motor domains.
W. O. Hancock and J. Howard (1999)
PNAS 96, 13147-13152
   Abstract »    Full Text »    PDF »
Reconstitution of Membrane Transport Powered by a Novel Dimeric Kinesin Motor of the Unc104/Kif1a Family Purified from Dictyostelium.
N. Pollock, E. L. de Hostos, C. W. Turck, and R. D. Vale (1999)
J. Cell Biol. 147, 493-506
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)