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Science 12 February 1999:
Vol. 283. no. 5404, pp. 996 - 998
DOI: 10.1126/science.283.5404.996
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Reports

A Copper Cofactor for the Ethylene Receptor ETR1 from Arabidopsis

Fernando I. Rodríguez, Jeffrey J. Esch, Anne E. Hall, Brad M. Binder, G. Eric Schaller, * Anthony B. Bleecker dagger

The ETR1 receptor from Arabidopsis binds the gaseous hormone ethylene. A copper ion associated with the ethylene-binding domain is required for high-affinity ethylene-binding activity. A missense mutation in the domain that renders the plant insensitive to ethylene eliminates both ethylene binding and the interaction of copper with the receptor. A sequence from the genome of the cyanobacterium Synechocystis sp. strain 6803 that shows homology to the ethylene-binding domain of ETR1 encodes a functional ethylene-binding protein. On the basis of sequence conservation between the Arabidopsis and the cyanobacterial ethylene-binding domains and on in vitro mutagenesis of ETR1, a structural model for this copper-based ethylene sensor domain is presented.

Department of Botany, 430 Lincoln Drive, University of Wisconsin, Madison, WI 53706, USA.
*   Present address: Department of Biochemistry and Molecular Biology, 46 College Road, University of New Hampshire, Durham, NH 03824, USA.

dagger    To whom correspondence should be addressed. E-mail: bleecker{at}facstaff.wisc.edu

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