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Science 29 January 1999:
Vol. 283. no. 5402, pp. 693 - 695
DOI: 10.1126/science.283.5402.693
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Reports

Immortal Time: Circadian Clock Properties of Rat Suprachiasmatic Cell Lines

David J. Earnest, * Fong-Qi Liang, Matt Ratcliff, Vincent M. Cassone

Cell lines derived from the rat suprachiasmatic nucleus (SCN) were screened for circadian clock properties distinctive of the SCN in situ. Immortalized SCN cells generated robust rhythms in uptake of the metabolic marker 2-deoxyglucose and in their content of neurotrophins. The phase relationship between these rhythms in vitro was identical to that exhibited by the SCN in vivo. Transplantation of SCN cell lines, but not mesencephalic or fibroblast lines, restored the circadian activity rhythm in arrhythmic, SCN-lesioned rats. Thus, distinctive oscillator, pacemaker, and clock properties of the SCN are not only retained but also maintained in an appropriate circadian phase relationship by immortalized SCN progenitors.

D. J. Earnest, F.-Q. Liang, M. Ratcliff, Department of Human Anatomy and Medical Neurobiology, College of Medicine, Texas A&M University Health Science Center, College Station, TX 77843-1114, USA. V. M. Cassone, Department of Biology, Texas A&M University, College Station, TX 77843-3258, USA.
*   To whom correspondence should be addressed. E-mail: dearnest{at}tamu.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)