Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 22 January 1999:
Vol. 283. no. 5401, pp. 537 - 540
DOI: 10.1126/science.283.5401.537
Prev | Table of Contents | Next

Reports

A Tobacco Syntaxin with a Role in Hormonal Control of Guard Cell Ion Channels

Barbara Leyman, Danny Geelen, Francisco J. Quintero, * Michael R. Blatt dagger

The plant hormone abscisic acid (ABA) regulates potassium and chloride ion channels at the plasma membrane of guard cells, leading to stomatal closure that reduces transpirational water loss from the leaf. The tobacco Nt-SYR1 gene encodes a syntaxin that is associated with the plasma membrane. Syntaxins and related SNARE proteins aid intracellular vesicle trafficking, fusion, and secretion. Disrupting Nt-Syr1 function by cleavage with Clostridium botulinum type C toxin or competition with a soluble fragment of Nt-Syr1 prevents potassium and chloride ion channel response to ABA in guard cells and implicates Nt-Syr1 in an ABA-signaling cascade.

Laboratory of Plant Physiology and Biophysics, University of London, Wye College, Wye, Kent TN25 5AH, UK.
*   Present address: Instituto de Recursos Naturales y Agrobiologia, Consejo Superior de Investigaciones Cientificas, Post Office Box 1052, Sevilla 41089, Spain.

dagger    To whom correspondence should be addressed. E-mail: mblatt{at}wye.ac.uk

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Dynamic Aspects of Ion Accumulation by Vesicle Traffic Under Salt Stress in Arabidopsis.
K. Hamaji, M. Nagira, K. Yoshida, M. Ohnishi, Y. Oda, T. Uemura, T. Goh, M. H. Sato, M. T. Morita, M. Tasaka, et al. (2009)
Plant Cell Physiol. 50, 2023-2033
   Abstract »    Full Text »    PDF »
A Tripartite SNARE-K+ Channel Complex Mediates in Channel-Dependent K+ Nutrition in Arabidopsis.
A. Honsbein, S. Sokolovski, C. Grefen, P. Campanoni, R. Pratelli, M. Paneque, Z. Chen, I. Johansson, and M. R. Blatt (2009)
PLANT CELL 21, 2859-2877
   Abstract »    Full Text »    PDF »
The guard cell as a single-cell model towards understanding drought tolerance and abscisic acid action.
C. Sirichandra, A. Wasilewska, F. Vlad, C. Valon, and J. Leung (2009)
J. Exp. Bot. 60, 1439-1463
   Abstract »    Full Text »    PDF »
Differential Expression Control and Polarized Distribution of Plasma Membrane-Resident SYP1 SNAREs in Arabidopsis thaliana.
K. Enami, M. Ichikawa, T. Uemura, N. Kutsuna, S. Hasezawa, T. Nakagawa, A. Nakano, and M. H. Sato (2009)
Plant Cell Physiol. 50, 280-289
   Abstract »    Full Text »    PDF »
The role of Rab GTPases in cell wall metabolism.
G. Lycett (2008)
J. Exp. Bot. 59, 4061-4074
   Abstract »    Full Text »    PDF »
A SNARE Complex Unique to Seed Plants Is Required for Protein Storage Vacuole Biogenesis and Seed Development of Arabidopsis thaliana.
K. Ebine, Y. Okatani, T. Uemura, T. Goh, K. Shoda, M. Niihama, M. T. Morita, C. Spitzer, M. S. Otegui, A. Nakano, et al. (2008)
PLANT CELL 20, 3006-3021
   Abstract »    Full Text »    PDF »
SNAREs: Cogs and Coordinators in Signaling and Development.
D. C. Bassham and M. R. Blatt (2008)
Plant Physiology 147, 1504-1515
   Full Text »    PDF »
Tomato Rab11a Characterization Evidenced a Difference Between SYP121-Dependent and SYP122-Dependent exocytosis.
R. U. Rehman, E. Stigliano, G. W. Lycett, L. Sticher, F. Sbano, M. Faraco, G. Dalessandro, and G.-P. Di Sansebastiano (2008)
Plant Cell Physiol. 49, 751-766
   Abstract »    Full Text »    PDF »
Functional Interaction of the SNARE Protein NtSyp121 in Ca2+ Channel Gating, Ca2+ Transients and ABA Signalling of Stomatal Guard Cells.
S. Sokolovski, A. Hills, R. A. Gay, and M. R. Blatt (2008)
Mol Plant 1, 347-358
   Abstract »    Full Text »    PDF »
Phylogeny of endocytic components yields insight into the process of nonendosymbiotic organelle evolution.
J. B. Dacks, P. P. Poon, and M. C. Field (2008)
PNAS 105, 588-593
   Abstract »    Full Text »    PDF »
Membrane trafficking and polar growth in root hairs and pollen tubes.
P. Campanoni and M. R. Blatt (2007)
J. Exp. Bot. 58, 65-74
   Abstract »    Full Text »    PDF »
Ca2+-Dependent and -Independent Abscisic Acid Activation of Plasma Membrane Anion Channels in Guard Cells of Nicotiana tabacum.
H. Marten, K. R. Konrad, P. Dietrich, M. R. G. Roelfsema, and R. Hedrich (2007)
Plant Physiology 143, 28-37
   Abstract »    Full Text »    PDF »
Proteomics of Plant Detergent-resistant Membranes.
J. Morel, S. Claverol, S. Mongrand, F. Furt, J. Fromentin, J.-J. Bessoule, J.-P. Blein, and F. Simon-Plas (2006)
Mol. Cell. Proteomics 5, 1396-1411
   Abstract »    Full Text »    PDF »
Functional Characterization of Ice Plant SKD1, an AAA-Type ATPase Associated with the Endoplasmic Reticulum-Golgi Network, and Its Role in Adaptation to Salt Stress.
Y. Jou, C.-P. Chiang, G.-Y. Jauh, and H. E. Yen (2006)
Plant Physiology 141, 135-146
   Abstract »    Full Text »    PDF »
Selective Mobility and Sensitivity to SNAREs Is Exhibited by the Arabidopsis KAT1 K+ Channel at the Plasma Membrane.
J.-U. Sutter, P. Campanoni, M. Tyrrell, and M. R. Blatt (2006)
PLANT CELL 18, 935-954
   Abstract »    Full Text »    PDF »
Rapid Phosphorylation of a Syntaxin during the Avr9/Cf-9-Race-Specific Signaling Pathway.
A. Heese, A. A. Ludwig, and J. D.G. Jones (2005)
Plant Physiology 138, 2406-2416
   Abstract »    Full Text »    PDF »
Mutations in the Arabidopsis Phosphoinositide Phosphatase Gene SAC9 Lead to Overaccumulation of PtdIns(4,5)P2 and Constitutive Expression of the Stress-Response Pathway.
M. E. Williams, J. Torabinejad, E. Cohick, K. Parker, E. J. Drake, J. E. Thompson, M. Hortter, and D. B. DeWald (2005)
Plant Physiology 138, 686-700
   Abstract »    Full Text »    PDF »
Seeing 'cool' and 'hot'--infrared thermography as a tool for non-invasive, high-throughput screening of Arabidopsis guard cell signalling mutants.
Y. Wang, G. Holroyd, A. M. Hetherington, and C. K-Y. Ng (2004)
J. Exp. Bot. 55, 1187-1193
   Abstract »    Full Text »    PDF »
Regulation of K+ channel activities in plants: from physiological to molecular aspects.
I. Cherel (2004)
J. Exp. Bot. 55, 337-351
   Abstract »    Full Text »    PDF »
A Plasma Membrane Syntaxin Is Phosphorylated in Response to the Bacterial Elicitor Flagellin.
T. S. Nuhse, T. Boller, and S. C. Peck (2003)
J. Biol. Chem. 278, 45248-45254
   Abstract »    Full Text »    PDF »
ABA-Activated SnRK2 Protein Kinase is Required for Dehydration Stress Signaling in Arabidopsis.
R. Yoshida, T. Hobo, K. Ichimura, T. Mizoguchi, F. Takahashi, J. Aronso, J. R. Ecker, and K. Shinozaki (2002)
Plant Cell Physiol. 43, 1473-1483
   Abstract »    Full Text »    PDF »
OSM1/SYP61: A Syntaxin Protein in Arabidopsis Controls Abscisic Acid-Mediated and Non-Abscisic Acid-Mediated Responses to Abiotic Stress.
J. Zhu, Z. Gong, C. Zhang, C.-P. Song, B. Damsz, G. Inan, H. Koiwa, J.-K. Zhu, P. M. Hasegawa, and R. A. Bressan (2002)
PLANT CELL 14, 3009-3028
   Abstract »    Full Text »    PDF »
Arabidopsis OST1 Protein Kinase Mediates the Regulation of Stomatal Aperture by Abscisic Acid and Acts Upstream of Reactive Oxygen Species Production.
A.-C. Mustilli, S. Merlot, A. Vavasseur, F. Fenzi, and J. Giraudat (2002)
PLANT CELL 14, 3089-3099
   Abstract »    Full Text »    PDF »
Cross-Talk in Abscisic Acid Signaling.
N. V. Fedoroff (2002)
Sci. STKE 2002, re10
   Abstract »    Full Text »    PDF »
Physical and Functional Interaction of the Arabidopsis K+ Channel AKT2 and Phosphatase AtPP2CA.
I. Cherel, E. Michard, N. Platet, K. Mouline, C. Alcon, H. Sentenac, and J.-B. Thibaud (2002)
PLANT CELL 14, 1133-1146
   Abstract »    Full Text »    PDF »
Specificity and Cross-Talk in Plant Signal Transduction: January 2002 Keystone Symposium.
N. A. Eckardt (2002)
PLANT CELL 14, S9-14
   Full Text »    PDF »
Abscisic Acid Signaling in Seeds and Seedlings.
R. R. Finkelstein, S. S. L. Gampala, and C. D. Rock (2002)
PLANT CELL 14, S15-45
   Full Text »    PDF »
Novel syntaxin gene sequences from Giardia, Trypanosoma and algae: implications for the ancient evolution of the eukaryotic endomembrane system.
J. B. Dacks and W. F. Doolittle (2002)
J. Cell Sci. 115, 1635-1642
   Abstract »    Full Text »    PDF »
The Abscisic Acid-Related SNARE Homolog NtSyr1 Contributes to Secretion and Growth: Evidence from Competition with Its Cytosolic Domain.
D. Geelen, B. Leyman, H. Batoko, G.-P. Di Sansebastiano, I. Moore, and M. R. Blatt (2002)
PLANT CELL 14, 387-406
   Abstract »    Full Text »    PDF »
Combining Genetics and Cell Biology to Crack the Code of Plant Cell Calcium Signaling.
G. J. Allen and J. I. Schroeder (2001)
Sci. STKE 2001, re13
   Abstract »    Full Text »    PDF »
Regulation of the Arabidopsis Transcriptome by Oxidative Stress.
R. Desikan, S. A.-H.-Mackerness, J. T. Hancock, and S. J. Neill (2001)
Plant Physiology 127, 159-172
   Abstract »    Full Text »    PDF »
Inactivation of AtRac1 by abscisic acid is essential for stomatal closure.
E. Lemichez, Y. Wu, J.-P. Sanchez, A. Mettouchi, J. Mathur, and N.-H. Chua (2001)
Genes & Dev. 15, 1808-1816
   Abstract »    Full Text »    PDF »
Direct Interaction of a Brain Voltage-Gated K+ Channel with Syntaxin 1A: Functional Impact on Channel Gating.
O. Fili, I. Michaelevski, Y. Bledi, D. Chikvashvili, D. Singer-Lahat, H. Boshwitz, M. Linial, and I. Lotan (2001)
J. Neurosci. 21, 1964-1974
   Abstract »    Full Text »    PDF »
The Arabidopsis Genome. An Abundance of Soluble N-Ethylmaleimide-Sensitive Factor Adaptor Protein Receptors.
A. A. Sanderfoot, F. F. Assaad, and N. V. Raikhel (2000)
Plant Physiology 124, 1558-1569
   Abstract »    Full Text »
Distinct Abscisic Acid Signaling Pathways for Modulation of Guard Cell versus Mesophyll Cell Potassium Channels Revealed by Expression Studies in Xenopus laevis Oocytes.
F. Sutton, S. S. Paul, X.-Q. Wang, and S. M. Assmann (2000)
Plant Physiology 124, 223-230
   Abstract »    Full Text »
AtVPS45 Complex Formation at the trans-Golgi Network.
D. C. Bassham, A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel (2000)
Mol. Biol. Cell 11, 2251-2265
   Abstract »    Full Text »
CFTR Chloride Channels: Binding Partners and Regulatory Networks.
A. P. Naren and K. L. Kirk (2000)
Physiology 15, 57-61
   Abstract »    Full Text »    PDF »
The t-SNARE AtVAM3p Resides on the Prevacuolar Compartment in Arabidopsis Root Cells.
A. A. Sanderfoot, V. Kovaleva, H. Zheng, and N. V. Raikhel (1999)
Plant Physiology 121, 929-938
   Abstract »    Full Text »
Exocytosis and Endocytosis.
N. H. Battey, N. C. James, A. J. Greenland, and C. Brownlee (1999)
PLANT CELL 11, 643-660
   Full Text »
The Transmembrane Domain of Syntaxin 1A Is Critical for Cytoplasmic Domain Protein-Protein Interactions.
J. L. Lewis, M. Dong, C. A. Earles, and E. R. Chapman (2001)
J. Biol. Chem. 276, 15458-15465
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)