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Science 1 January 1999:
Vol. 283. no. 5398, pp. 94 - 98
DOI: 10.1126/science.283.5398.94
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Reports

Plant Paralog to Viral Movement Protein That Potentiates Transport of mRNA into the Phloem

Beatriz Xoconostle-Cázares, * Yu Xiang, * Roberto Ruiz-Medrano, * Hong-Li Wang, Jan Monzer, Byung-Chun Yoo, K. C. McFarland, Vincent R. Franceschi, William J. Lucas dagger

CmPP16 from Cucurbita maxima was cloned and the protein was shown to possess properties similar to those of viral movement proteins. CmPP16 messenger RNA (mRNA) is present in phloem tissue, whereas protein appears confined to sieve elements (SE). Microinjection and grafting studies revealed that CmPP16 moves from cell to cell, mediates the transport of sense and antisense RNA, and moves together with its mRNA into the SE of scion tissue. CmPP16 possesses the characteristics that are likely required to mediate RNA delivery into the long-distance translocation stream. Thus, RNA may move within the phloem as a component of a plant information superhighway.

B. Xoconostle-Cázares, Y. Xiang, R. Ruiz-Medrano, H.-L. Wang, J. Monzer, B.-C. Yoo, K. C. McFarland, W. J. Lucas, Section of Plant Biology, Division of Biological Sciences, University of California, Davis, CA 95616, USA. V. R. Franceschi, Electron Microscopy Center, Washington State University, Pullman, WA 99164-4210, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: wjlucas{at}ucdavis.edu

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