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Science 1 January 1999:
Vol. 283. no. 5398, pp. 67 - 70
DOI: 10.1126/science.283.5398.67
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Reports

Requirement for Diverse, Low-Abundance Peptides in Positive Selection of T Cells

Gregory M. Barton, Alexander Y. Rudensky *

Whether a single major histocompatibility complex (MHC)-bound peptide can drive the positive selection of large numbers of T cells has been a controversial issue. A diverse population of self peptides was shown to be essential for the in vivo development of CD4 T cells. Mice in which all but 5 percent of MHC class II molecules were bound by a single peptide had wild-type numbers of CD4 T cells. However, when the diversity within this 5 percent was lost, CD4 T cell development was impaired. Blocking the major peptide-MHC complex in thymus organ culture had no effect on T cell development, indicating that positive selection occurred on the diverse peptides present at low levels. This requirement for peptide diversity indicates that the interaction between self peptides and T cell receptors during positive selection is highly specific.

G. M. Barton, Molecular and Cellular Biology Program of the University of Washington and Fred Hutchinson Cancer Research Center, Seattle, WA 98195, USA, and Department of Immunology, University of Washington School of Medicine, Seattle, WA 98195, USA. A. Y. Rudensky, Department of Immunology and Howard Hughes Medical Institute, University of Washington School of Medicine, Seattle, WA 98195, USA.
*   To whom correspondence should be addressed at Howard Hughes Medical Institute, University of Washington, Box 357370, Seattle, WA 98195, USA. E-mail: sasha{at}nucleus.immunol.washington.edu

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Science. ISSN 0036-8075 (print), 1095-9203 (online)