Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 18 December 1998:
Vol. 282. no. 5397, pp. 2220 - 2226
DOI: 10.1126/science.282.5397.2220
Prev | Table of Contents | Next

Research Articles

Structure of the MscL Homolog from Mycobacterium tuberculosis: A Gated Mechanosensitive Ion Channel

Geoffrey Chang, * Robert H. Spencer, * Allen T. Lee, Margaret T. Barclay, Douglas C. Rees dagger

Mechanosensitive ion channels play a critical role in transducing physical stresses at the cell membrane into an electrochemical response. The MscL family of large-conductance mechanosensitive channels is widely distributed among prokaryotes and may participate in the regulation of osmotic pressure changes within the cell. In an effort to better understand the structural basis for the function of these channels, the structure of the MscL homolog from Mycobacterium tuberculosis was determined by x-ray crystallography to 3.5 angstroms resolution. This channel is organized as a homopentamer, with each subunit containing two transmembrane alpha  helices and a third cytoplasmic alpha  helix. From the extracellular side, a water-filled opening approximately 18 angstroms in diameter leads into a pore lined with hydrophilic residues which narrows at the cytoplasmic side to an occluded hydrophobic apex that may act as the channel gate. This structure may serve as a model for other mechanosensitive channels, as well as the broader class of pentameric ligand-gated ion channels exemplified by the nicotinic acetylcholine receptor.

Howard Hughes Medical Institute and Division of Chemistry and Chemical Engineering, 147-75CH, California Institute of Technology, Pasadena, CA 91125, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: dcrees{at}caltech.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
State-stabilizing Interactions in Bacterial Mechanosensitive Channel Gating and Adaptation.
A. Anishkin and S. Sukharev (2009)
J. Biol. Chem. 284, 19153-19157
   Abstract »    Full Text »    PDF »
An open-pore structure of the mechanosensitive channel MscL derived by determining transmembrane domain interactions upon gating.
Y. Li, R. Wray, C. Eaton, and P. Blount (2009)
FASEB J 23, 2197-2204
   Abstract »    Full Text »    PDF »
Three-dimensional structure of the human copper transporter hCTR1.
C. J. De Feo, S. G. Aller, G. S. Siluvai, N. J. Blackburn, and V. M. Unger (2009)
PNAS 106, 4237-4242
   Abstract »    Full Text »    PDF »
Loop Gating of Connexin Hemichannels Involves Movement of Pore-lining Residues in the First Extracellular Loop Domain.
V. K. Verselis, M. P. Trelles, C. Rubinos, T. A. Bargiello, and M. Srinivas (2009)
J. Biol. Chem. 284, 4484-4493
   Abstract »    Full Text »    PDF »
Detergent binding explains anomalous SDS-PAGE migration of membrane proteins.
A. Rath, M. Glibowicka, V. G. Nadeau, G. Chen, and C. M. Deber (2009)
PNAS 106, 1760-1765
   Abstract »    Full Text »    PDF »
Proteome-Based Comparative Analyses of Growth Stages Reveal New Cell Cycle-Dependent Functions in the Predatory Bacterium Bdellovibrio bacteriovorus.
M. Dori-Bachash, B. Dassa, S. Pietrokovski, and E. Jurkevitch (2008)
Appl. Envir. Microbiol. 74, 7152-7162
   Abstract »    Full Text »    PDF »
Ion Channels in Microbes.
B. Martinac, Y. Saimi, and C. Kung (2008)
Physiol Rev 88, 1449-1490
   Abstract »    Full Text »    PDF »
The Structure of an Open Form of an E. coli Mechanosensitive Channel at 3.45 A Resolution.
W. Wang, S. S. Black, M. D. Edwards, S. Miller, E. L. Morrison, W. Bartlett, C. Dong, J. H. Naismith, and I. R. Booth (2008)
Science 321, 1179-1183
   Abstract »    Full Text »    PDF »
A Structural Mechanism for MscS Gating in Lipid Bilayers.
V. Vasquez, M. Sotomayor, J. Cordero-Morales, K. Schulten, and E. Perozo (2008)
Science 321, 1210-1214
   Abstract »    Full Text »    PDF »
Mechanosensitive Channel MscS in the Open State: Modeling of the Transition, Explicit Simulations, and Experimental Measurements of Conductance.
A. Anishkin, K. Kamaraju, and S. Sukharev (2008)
J. Gen. Physiol. 132, 67-83
   Abstract »    Full Text »    PDF »
Responses of Bacillus subtilis to Hypotonic Challenges: Physiological Contributions of Mechanosensitive Channels to Cellular Survival.
T. Hoffmann, C. Boiangiu, S. Moses, and E. Bremer (2008)
Appl. Envir. Microbiol. 74, 2454-2460
   Abstract »    Full Text »    PDF »
Gating-associated conformational changes in the mechanosensitive channel MscL.
K. Yoshimura, J. Usukura, and M. Sokabe (2008)
PNAS 105, 4033-4038
   Abstract »    Full Text »    PDF »
An integrative approach to understanding mechanosensation.
C. C. Poirier and P. A. Iglesias (2007)
Brief Bioinform 8, 258-265
   Abstract »    Full Text »    PDF »
Tryptophan-scanning Mutagenesis in the {alpha}M3 Transmembrane Domain of the Muscle-type Acetylcholine Receptor: A SPRING MODEL REVEALED.
J. D. Otero-Cruz, C. A. Baez-Pagan, I. M. Caraballo-Gonzalez, and J. A. Lasalde-Dominicci (2007)
J. Biol. Chem. 282, 9162-9171
   Abstract »    Full Text »    PDF »
Gain-of-Function Mutations in the MEC-4 DEG/ENaC Sensory Mechanotransduction Channel Alter Gating and Drug Blockade.
A. L. Brown, S. M. Fernandez-Illescas, Z. Liao, and M. B. Goodman (2007)
J. Gen. Physiol. 129, 161-173
   Abstract »    Full Text »    PDF »
Nanoliter microfluidic hybrid method for simultaneous screening and optimization validated with crystallization of membrane proteins.
L. Li, D. Mustafi, Q. Fu, V. Tereshko, D. L. Chen, J. D. Tice, and R. F. Ismagilov (2006)
PNAS 103, 19243-19248
   Abstract »    Full Text »    PDF »
An approach to crystallizing proteins by synthetic symmetrization.
D. R. Banatao, D. Cascio, C. S. Crowley, M. R. Fleissner, H. L. Tienson, and T. O. Yeates (2006)
PNAS 103, 16230-16235
   Abstract »    Full Text »    PDF »
State-dependent Conformations of the Translocation Pathway in the Tyrosine Transporter Tyt1, a Novel Neurotransmitter:Sodium Symporter from Fusobacterium nucleatum.
M. Quick, H. Yano, N. R. Goldberg, L. Duan, T. Beuming, L. Shi, H. Weinstein, and J. A. Javitch (2006)
J. Biol. Chem. 281, 26444-26454
   Abstract »    Full Text »    PDF »
Projection structure of the human copper transporter CTR1 at 6-A resolution reveals a compact trimer with a novel channel-like architecture.
S. G. Aller and V. M. Unger (2006)
PNAS 103, 3627-3632
   Abstract »    Full Text »    PDF »
Lateral mobility of proteins in liquid membranes revisited.
Y. Gambin, R. Lopez-Esparza, M. Reffay, E. Sierecki, N. S. Gov, M. Genest, R. S. Hodges, and W. Urbach (2006)
PNAS 103, 2098-2102
   Abstract »    Full Text »    PDF »
Transmembrane glycine zippers: Physiological and pathological roles in membrane proteins.
S. Kim, T.-J. Jeon, A. Oberai, D. Yang, J. J. Schmidt, and J. U. Bowie (2005)
PNAS 102, 14278-14283
   Abstract »    Full Text »    PDF »
A Light-Actuated Nanovalve Derived from a Channel Protein.
A. Kocer, M. Walko, W. Meijberg, and B. L. Feringa (2005)
Science 309, 755-758
   Abstract »    Full Text »    PDF »
An Angstrom Scale Interaction between Plasma Membrane ATP-Gated P2X2 and {alpha}4{beta}2 Nicotinic Channels Measured with Fluorescence Resonance Energy Transfer and Total Internal Reflection Fluorescence Microscopy.
B. S. Khakh, J. A. Fisher, R. Nashmi, D. N. Bowser, and H. A. Lester (2005)
J. Neurosci. 25, 6911-6920
   Abstract »    Full Text »    PDF »
Secondary Structure and Gating Rearrangements of Transmembrane Segments in Rat P2X4 Receptor Channels.
S. D. Silberberg, T.-H. Chang, and K. J. Swartz (2005)
J. Gen. Physiol. 125, 347-359
   Abstract »    Full Text »    PDF »
Lactococcus lactis Uses MscL as Its Principal Mechanosensitive Channel.
J. H. A. Folgering, P. C. Moe, G. K. Schuurman-Wolters, P. Blount, and B. Poolman (2005)
J. Biol. Chem. 280, 8784-8792
   Abstract »    Full Text »    PDF »
Mutations in the Pore Region Modify Epithelial Sodium Channel Gating by Shear Stress.
M. D. Carattino, S. Sheng, and T. R. Kleyman (2005)
J. Biol. Chem. 280, 4393-4401
   Abstract »    Full Text »    PDF »
Gain-of-function Mutations Reveal Expanded Intermediate States and a Sequential Action of Two Gates in MscL.
A. Anishkin, C.-S. Chiang, and S. Sukharev (2005)
J. Gen. Physiol. 125, 155-170
   Abstract »    Full Text »    PDF »
Eukaryotic CTR Copper Uptake Transporters Require Two Faces of the Third Transmembrane Domain for Helix Packing, Oligomerization, and Function.
S. G. Aller, E. T. Eng, C. J. De Feo, and V. M. Unger (2004)
J. Biol. Chem. 279, 53435-53441
   Abstract »    Full Text »    PDF »
Time-Resolved Measurement of State-Specific P2X2 Ion Channel Cytosolic Gating Motions.
J. A. Fisher, G. Girdler, and B. S. Khakh (2004)
J. Neurosci. 24, 10475-10487
   Abstract »    Full Text »    PDF »
Genetic Models of Mechanotransduction: The Nematode Caenorhabditis elegans.
P. Syntichaki and N. Tavernarakis (2004)
Physiol Rev 84, 1097-1153
   Abstract »    Full Text »    PDF »
Phylogenetic conservation of disulfide-linked, dimeric acetylcholine receptor pentamers in southern ocean electric rays.
M. L. Tierney, K. E. Osborn, P. J. Milburn, M. H. B. Stowell, and S. M. Howitt (2004)
J. Exp. Biol. 207, 3581-3590
   Abstract »    Full Text »    PDF »
Computational analysis of {alpha}-helical membrane protein structure: implications for the prediction of 3D structural models.
T. A. Eyre, L. Partridge, and J. M. Thornton (2004)
Protein Eng. Des. Sel. 17, 613-624
   Abstract »    Full Text »    PDF »
Gating Transitions in Bacterial Ion Channels Measured at 3 {micro}s Resolution.
G. Shapovalov and H. A. Lester (2004)
J. Gen. Physiol. 124, 151-161
   Abstract »    Full Text »    PDF »
The CorA Mg2+ Transporter Is a Homotetramer.
M. A. Warren, L. M. Kucharski, A. Veenstra, L. Shi, P. F. Grulich, and M. E. Maguire (2004)
J. Bacteriol. 186, 4605-4612
   Abstract »    Full Text »    PDF »
An in vivo assay identifies changes in residue accessibility on mechanosensitive channel gating.
J. L. Bartlett, G. Levin, and P. Blount (2004)
PNAS 101, 10161-10165
   Abstract »    Full Text »    PDF »
Cell mechanics and mechanotransduction: pathways, probes, and physiology.
H. Huang, R. D. Kamm, and R. T. Lee (2004)
Am J Physiol Cell Physiol 287, C1-C11
   Abstract »    Full Text »    PDF »
Mechanosensitive ion channels: molecules of mechanotransduction.
B. Martinac (2004)
J. Cell Sci. 117, 2449-2460
   Abstract »    Full Text »    PDF »
Regulation of Sodium Channel Function by Bilayer Elasticity: The Importance of Hydrophobic Coupling. Effects of Micelle-forming Amphiphiles and Cholesterol.
J. A. Lundbaek, P. Birn, A. J. Hansen, R. Sogaard, C. Nielsen, J. Girshman, M. J. Bruno, S. E. Tape, J. Egebjerg, D. V. Greathouse, et al. (2004)
J. Gen. Physiol. 123, 599-621
   Abstract »    Full Text »    PDF »
Total chemical synthesis and electrophysiological characterization of mechanosensitive channels from Escherichia coli and Mycobacterium tuberculosis.
D. Clayton, G. Shapovalov, J. A. Maurer, D. A. Dougherty, H. A. Lester, and G. G. Kochendoerfer (2004)
PNAS 101, 4764-4769
   Abstract »    Full Text »    PDF »
Analytic models for mechanotransduction: Gating a mechanosensitive channel.
P. Wiggins and R. Phillips (2004)
PNAS 101, 4071-4076
   Abstract »    Full Text »    PDF »
Structure of the multidrug resistance efflux transporter EmrE from Escherichia coli.
C. Ma and G. Chang (2004)
PNAS 101, 2852-2857
   Abstract »    Full Text »    PDF »
From the Cover: Computational design of water-soluble analogues of the potassium channel KcsA.
A. M. Slovic, H. Kono, J. D. Lear, J. G. Saven, and W. F. DeGrado (2004)
PNAS 101, 1828-1833
   Abstract »    Full Text »    PDF »
Mechanosensitive Channels: Multiplicity of Families and Gating Paradigms.
S. Sukharev and D. P. Corey (2004)
Sci. STKE 2004, re4
   Abstract »    Full Text »    PDF »
Design and synthesis of antituberculars: preparation and evaluation against Mycobacterium tuberculosis of an isoniazid Schiff base.
M. J. Hearn and M. H. Cynamon (2004)
J. Antimicrob. Chemother. 53, 185-191
   Abstract »    Full Text »    PDF »
A role for mechanosensitive channels in survival of stationary phase: Regulation of channel expression by RpoS.
N. R. Stokes, H. D. Murray, C. Subramaniam, R. L. Gourse, P. Louis, W. Bartlett, S. Miller, and I. R. Booth (2003)
PNAS 100, 15959-15964
   Abstract »    Full Text »    PDF »
Sites of Excitatory and Inhibitory Actions of Alcohols on Neuronal {alpha}2{beta}4 Nicotinic Acetylcholine Receptors.
C. M. Borghese, L. A. Henderson, V. Bleck, J. R. Trudell, and R. A. Harris (2003)
J. Pharmacol. Exp. Ther. 307, 42-52
   Abstract »    Full Text »    PDF »
Inhaled Anesthetics and Immobility: Mechanisms, Mysteries, and Minimum Alveolar Anesthetic Concentration.
J. M. Sonner, J. F. Antognini, R. C. Dutton, P. Flood, A. T. Gray, R. A. Harris, G. E. Homanics, J. Kendig, B. Orser, D. E. Raines, et al. (2003)
Anesth. Analg. 97, 718-740
   Abstract »    Full Text »    PDF »
The Closed Structure of the MscS Mechanosensitive Channel: CROSS-LINKING OF SINGLE CYSTEINE MUTANTS.
S. Miller, M. D. Edwards, C. Ozdemir, and I. R. Booth (2003)
J. Biol. Chem. 278, 32246-32250
   Abstract »    Full Text »    PDF »
Structure and Mechanism of the Glycerol-3-Phosphate Transporter from Escherichia coli.
Y. Huang, M. J. Lemieux, J. Song, M. Auer, and D.-N. Wang (2003)
Science 301, 616-620
   Abstract »    Full Text »    PDF »
Crystal Structure of the Potassium Channel KirBac1.1 in the Closed State.
A. Kuo, J. M. Gulbis, J. F. Antcliff, T. Rahman, E. D. Lowe, J. Zimmer, J. Cuthbertson, F. M. Ashcroft, T. Ezaki, and D. A. Doyle (2003)
Science 300, 1922-1926
   Abstract »    Full Text »    PDF »
Liquid-vapor oscillations of water in hydrophobic nanopores.
O. Beckstein and M. S. P. Sansom (2003)
PNAS 100, 7063-7068
   Abstract »    Full Text »    PDF »
The transient receptor potential channel on the yeast vacuole is mechanosensitive.
X.-L. Zhou, A. F. Batiza, S. H. Loukin, C. P. Palmer, C. Kung, and Y. Saimi (2003)
PNAS 100, 7105-7110
   Abstract »    Full Text »    PDF »
Generation and Evaluation of a Large Mutational Library from the Escherichia coli Mechanosensitive Channel of Large Conductance, MscL: IMPLICATIONS FOR CHANNEL GATING AND EVOLUTIONARY DESIGN.
J. A. Maurer and D. A. Dougherty (2003)
J. Biol. Chem. 278, 21076-21082
   Abstract »    Full Text »    PDF »
C Termini of the Escherichia coli Mechanosensitive Ion Channel (MscS) Move Apart upon the Channel Opening.
P. Koprowski and A. Kubalski (2003)
J. Biol. Chem. 278, 11237-11245
   Abstract »    Full Text »    PDF »
Two Families of Mechanosensitive Channel Proteins.
C. D. Pivetti, M.-R. Yen, S. Miller, W. Busch, Y.-H. Tseng, I. R. Booth, and M. H. Saier Jr. (2003)
Microbiol. Mol. Biol. Rev. 67, 66-85
   Abstract »    Full Text »    PDF »
On the Conformation of the COOH-terminal Domain of the Large Mechanosensitive Channel MscL.
A. Anishkin, V. Gendel, N. A. Sharifi, C.-S. Chiang, L. Shirinian, H. R. Guy, and S. Sukharev (2003)
J. Gen. Physiol. 121, 227-244
   Abstract »    Full Text »    PDF »
Proteomics of Synechocystis sp. Strain PCC 6803: Identification of Plasma Membrane Proteins.
F. Huang, I. Parmryd, F. Nilsson, A. L. Persson, H. B. Pakrasi, B. Andersson, and B. Norling (2002)
Mol. Cell. Proteomics 1, 956-966
   Abstract »    Full Text »    PDF »
Crystal Structure of Escherichia coli MscS, a Voltage-Modulated and Mechanosensitive Channel.
R. B. Bass, P. Strop, M. Barclay, and D. C. Rees (2002)
Science 298, 1582-1587
   Abstract »    Full Text »    PDF »
Family ties of gated pores: evolution of the sensor module.
A. KUMANOVICS, G. LEVIN, and P. BLOUNT (2002)
FASEB J 16, 1623-1629
   Abstract »    Full Text »    PDF »
Molecular Physiology of P2X Receptors.
R. A. North (2002)
Physiol Rev 82, 1013-1067
   Abstract »    Full Text »    PDF »
Solution Structure of Peptide Toxins That Block Mechanosensitive Ion Channels.
R. E. Oswald, T. M. Suchyna, R. McFeeters, P. Gottlieb, and F. Sachs (2002)
J. Biol. Chem. 277, 34443-34450
   Abstract »    Full Text »    PDF »
Passive water and urea permeability of a human Na+-glutamate cotransporter expressed in Xenopus oocytes.
N. MacAulay, U. Gether, D. A Klaerke, and T. Zeuthen (2002)
J. Physiol. 542, 817-828
   Abstract »    Full Text »    PDF »
Functional Design of Bacterial Mechanosensitive Channels. COMPARISONS AND CONTRASTS ILLUMINATED BY RANDOM MUTAGENESIS.
K. Okada, P. C. Moe, and P. Blount (2002)
J. Biol. Chem. 277, 27682-27688
   Abstract »    Full Text »    PDF »
Molecular modelling of specific and non-specific anaesthetic interactions.
J. R. Trudell and E. Bertaccini (2002)
Br. J. Anaesth. 89, 32-40
   Abstract »    Full Text »    PDF »
Predicting the transmembrane secondary structure of ligand-gated ion channels.
E. Bertaccini and J.R. Trudell (2002)
Protein Eng. Des. Sel. 15, 443-453
   Abstract »    Full Text »    PDF »
The E. coli BtuCD Structure: A Framework for ABC Transporter Architecture and Mechanism.
K. P. Locher, A. T. Lee, and D. C. Rees (2002)
Science 296, 1091-1098
   Abstract »    Full Text »    PDF »
Conformational pathways in the gating of Escherichia coli mechanosensitive channel.
Y. Kong, Y. Shen, T. E. Warth, and J. Ma (2002)
PNAS 99, 5999-6004
   Abstract »    Full Text »    PDF »
Gating the bacterial mechanosensitive channel MscL invivo.
A. F. Batiza, M. M.-C. Kuo, K. Yoshimura, and C. Kung (2002)
PNAS 99, 5643-5648
   Abstract »    Full Text »    PDF »
Gramicidin A channels switch between stretch activation and stretch inactivation depending on bilayer thickness.
B. Martinac and O. P. Hamill (2002)
PNAS 99, 4308-4312
   Abstract »    Full Text »    PDF »
Molecular Basis of Mechanotransduction in Living Cells.
O. P. Hamill and B. Martinac (2001)
Physiol Rev 81, 685-740
   Abstract »    Full Text »    PDF »
Structure of a Glycerol-Conducting Channel and the Basis for Its Selectivity.
D. Fu, A. Libson, L. J. W. Miercke, C. Weitzman, P. Nollert, J. Krucinski, and R. M. Stroud (2000)
Science 290, 481-486
   Abstract »    Full Text »
Effects of Organic Antagonists of Ca2+, Na+, and K+ on Chemotaxis and Motility of Escherichia coli.
L. S. Tisa, J. J. Sekelsky, and J. Adler (2000)
J. Bacteriol. 182, 4856-4861
   Abstract »    Full Text »
Effects of pore mutations and permeant ion concentration on the spontaneous gating activity of OmpC porin.
N. Liu, H. Samartzidou, K. W. Lee, J. M. Briggs, and A. H. Delcour (2000)
Protein Eng. Des. Sel. 13, 491-500
   Abstract »    Full Text »    PDF »
Internal packing of helical membrane proteins.
M. Eilers, S. C. Shekar, T. Shieh, S. O. Smith, and P. J. Fleming (2000)
PNAS 97, 5796-5801
   Abstract »    Full Text »    PDF »
Crystallographic Analyses of Ion Channels: Lessons and Challenges.
D. C. Rees, G. Chang, and R. H. Spencer (2000)
J. Biol. Chem. 275, 713-716
   Full Text »    PDF »
Contributions of the Different Extramembranous Domains of the Mechanosensitive Ion Channel MscL to Its Response to Membrane Tension.
B. Ajouz, C. Berrier, M. Besnard, B. Martinac, and A. Ghazi (2000)
J. Biol. Chem. 275, 1015-1022
   Abstract »    Full Text »    PDF »
Elongation Factor Tu and DnaK Are Transferred from the Cytoplasm to the Periplasm of Escherichia coli during Osmotic Downshock Presumably via the Mechanosensitive Channel MscL.
C. Berrier, A. Garrigues, G. Richarme, and A. Ghazi (2000)
J. Bacteriol. 182, 248-251
   Abstract »    Full Text »
Contributions of the C-terminal Domain to the Control of P2X Receptor Desensitization.
T.-a. Koshimizu, M. Koshimizu, and S. S. Stojilkovic (1999)
J. Biol. Chem. 274, 37651-37657
   Abstract »    Full Text »    PDF »
The CorA Mg2+ Transport Protein of Salmonella typhimurium. MUTAGENESIS OF CONSERVED RESIDUES IN THE SECOND MEMBRANE DOMAIN.
M. A. Szegedy and M. E. Maguire (1999)
J. Biol. Chem. 274, 36973-36979
   Abstract »    Full Text »    PDF »
Block of an ether-a-go-go-Like K+ Channel by Imipramine Rescues egl-2 Excitation Defects in Caenorhabditis elegans.
D. Weinshenker, A. Wei, L. Salkoff, and J. H. Thomas (1999)
J. Neurosci. 19, 9831-9840
   Abstract »    Full Text »    PDF »
Mechanical force-induced signal transduction in lung cells.
M. Liu, A. K. Tanswell, and M. Post (1999)
Am J Physiol Lung Cell Mol Physiol 277, L667-L683
   Abstract »    Full Text »    PDF »
Molecular Identification of a Eukaryotic, Stretch-Activated Nonselective Cation Channel.
M. Kanzaki, M. Nagasawa, I. Kojima, C. Sato, K. Naruse, M. Sokabe, and H. Iida (1999)
Science 285, 882-886
   Abstract »    Full Text »
Structural Rearrangements Underlying K+-Channel Activation Gating.
E. Perozo, D. Marien, Cortes, and L. G. Cuello (1999)
Science 285, 73-78
   Abstract »    Full Text »
Mechanosensitive channels in bacteria as membrane tension reporters.
S. SUKHAREV (1999)
FASEB J 13, 55-61
   Abstract »    Full Text »    PDF »
TREK-2, a New Member of the Mechanosensitive Tandem-pore K+ Channel Family.
H. Bang, Y. Kim, and D. Kim (2000)
J. Biol. Chem. 275, 17412-17419
   Abstract »    Full Text »    PDF »
Correlating a Protein Structure with Function of a Bacterial Mechanosensitive Channel.
P. C. Moe, G. Levin, and P. Blount (2000)
J. Biol. Chem. 275, 31121-31127
   Abstract »    Full Text »    PDF »
Comparing and Contrasting Escherichia coli and Mycobacterium tuberculosis Mechanosensitive Channels (MscL). NEW GAIN OF FUNCTION MUTATIONS IN THE LOOP REGION.
J. A. Maurer, D. E. Elmore, H. A. Lester, and D. A. Dougherty (2000)
J. Biol. Chem. 275, 22238-22244
   Abstract »    Full Text »    PDF »
Gramicidin A channels switch between stretch activation and stretch inactivation depending on bilayer thickness.
B. Martinac and O. P. Hamill (2002)
PNAS 99, 4308-4312
   Abstract »    Full Text »    PDF »
Conformational pathways in the gating of Escherichia coli mechanosensitive channel.
Y. Kong, Y. Shen, T. E. Warth, and J. Ma (2002)
PNAS 99, 5999-6004
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)