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Science 20 November 1998:
Vol. 282. no. 5393, pp. 1511 - 1516
DOI: 10.1126/science.282.5393.1511
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Reports

The Role of Far1p in Linking the Heterotrimeric G Protein to Polarity Establishment Proteins During Yeast Mating

Anne-Christine Butty, Peter M. Pryciak, Linda S. Huang, Ira Herskowitz, Matthias Peter *

Heterotrimeric guanosine triphosphate (GTP)-binding proteins (G proteins) determine tissue and cell polarity in a variety of organisms. In yeast, cells orient polarized growth toward the mating partner along a pheromone gradient by a mechanism that requires Far1p and Cdc24p. Far1p bound Gbeta gamma and interacted with polarity establishment proteins, which organize the actin cytoskeleton. Cells containing mutated Far1p unable to bind Gbeta gamma or polarity establishment proteins were defective for orienting growth toward their mating partner. In response to pheromones, Far1p moves from the nucleus to the cytoplasm. Thus, Far1p functions as an adaptor that recruits polarity establishment proteins to the site of extracellular signaling marked by Gbeta gamma to polarize assembly of the cytoskeleton in a morphogenetic gradient.

A.-C. Butty and M. Peter, Swiss Institute for Experimental Cancer Research (ISREC), Chemin des Boveresses 155, 1066 Epalinges/VD, Switzerland. P. M. Pryciak, Department of Molecular Genetics and Microbiology, University of Massachusetts Medical Center, Worcester Foundation Campus, 222 Maple Avenue, Shrewsbury, MA 01545, USA. L. S. Huang and I. Herskowitz, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143-0448, USA.
*   To whom correspondence should be addressed. E-mail: matthias.peter{at}isrec.unil.ch

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   Abstract »    Full Text »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »
Polarization of cell growth in yeast. I. Establishment and maintenance of polarity states.
D Pruyne and A Bretscher (2000)
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   Abstract »    PDF »
Details and Concerns Regarding the G2/M DNA Damage Checkpoint in Budding Yeast.
T. WEINERT, E. LITTLE, L. SHANKS, A. ADMIRE, R. GARDNER, C. PUTNAM, R. MICHELSON, K. NYBERG, and P. SUNDARESHAN (2000)
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   Abstract »    PDF »
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S. Lev, A. Sharon, R. Hadar, H. Ma, and B. A. Horwitz (1999)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »
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   Abstract »    Full Text »
A Cell's Sense of Direction.
C. A. Parent and P. N. Devreotes (1999)
Science 284, 765-770
   Abstract »    Full Text »
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A. Nern and R. A. Arkowitz (1999)
J. Cell Biol. 144, 1187-1202
   Abstract »    Full Text »    PDF »
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P. Dube, A. DeCostanzo, and J. B. Konopka (2000)
J. Biol. Chem. 275, 26492-26499
   Abstract »    Full Text »    PDF »
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I. Bose, J. E. Irazoqui, J. J. Moskow, E. S. G. Bardes, T. R. Zyla, and D. J. Lew (2001)
J. Biol. Chem. 276, 7176-7186
   Abstract »    Full Text »    PDF »
GTPgamma S Regulation of a 12-Transmembrane Guanylyl Cyclase Is Retained after Mutation to an Adenylyl Cyclase.
J. Roelofs, H. M. Loovers, and P. J. M. Van Haastert (2001)
J. Biol. Chem. 276, 40740-40745
   Abstract »    Full Text »    PDF »


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