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Science 6 November 1998:
Vol. 282. no. 5391, pp. 1145 - 1147
DOI: 10.1126/science.282.5391.1145
Prev | Table of Contents

Reports

Embryonic Stem Cell Lines Derived from Human Blastocysts

James A. Thomson, * Joseph Itskovitz-Eldor, Sander S. Shapiro, Michelle A. Waknitz, Jennifer J. Swiergiel, Vivienne S. Marshall, Jeffrey M. Jones

Human blastocyst-derived, pluripotent cell lines are described that have normal karyotypes, express high levels of telomerase activity, and express cell surface markers that characterize primate embryonic stem cells but do not characterize other early lineages. After undifferentiated proliferation in vitro for 4 to 5 months, these cells still maintained the developmental potential to form trophoblast and derivatives of all three embryonic germ layers, including gut epithelium (endoderm); cartilage, bone, smooth muscle, and striated muscle (mesoderm); and neural epithelium, embryonic ganglia, and stratified squamous epithelium (ectoderm). These cell lines should be useful in human developmental biology, drug discovery, and transplantation medicine.

J. A. Thomson, M. A. Waknitz, J. J. Swiergiel, V. S. Marshall, Wisconsin Regional Primate Research Center, University of Wisconsin, Madison, WI 53715, USA. J. Itskovitz-Eldor, Department of Obstetrics and Gynecology, Rambam Medical Center, Faculty of Medicine, Technion, Haifa 31096, Israel. S. S. Shapiro and J. M. Jones, Department of Obstetrics and Gynecology, University of Wisconsin, Madison, WI 53715, USA.
*   To whom correspondence should be addressed.

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In Search of the In Vivo Identity of Mesenchymal Stem Cells.
L. da Silva Meirelles, A. I. Caplan, and N. B. Nardi (2008)
Stem Cells 26, 2287-2299
   Abstract »    Full Text »    PDF »
Control of Human Embryonic Stem Cell Colony and Aggregate Size Heterogeneity Influences Differentiation Trajectories.
C. L. Bauwens, R. Peerani, S. Niebruegge, K. A. Woodhouse, E. Kumacheva, M. Husain, and P. W. Zandstra (2008)
Stem Cells 26, 2300-2310
   Abstract »    Full Text »    PDF »
Recombinant Vitronectin Is a Functionally Defined Substrate That Supports Human Embryonic Stem Cell Self-Renewal via {alpha}V{beta}5 Integrin.
S. R. Braam, L. Zeinstra, S. Litjens, D. Ward-van Oostwaard, S. van den Brink, L. van Laake, F. Lebrin, P. Kats, R. Hochstenbach, R. Passier, et al. (2008)
Stem Cells 26, 2257-2265
   Abstract »    Full Text »    PDF »
Stem Cell-Derived Therapeutic Myelin Repair Requires 7% Cell Replacement.
M. E. Kiel, C. P. Chen, D. Sadowski, and R. D. McKinnon (2008)
Stem Cells 26, 2229-2236
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Induced Pluripotent Stem Cells Generated from Patients with ALS Can Be Differentiated into Motor Neurons.
J. T. Dimos, K. T. Rodolfa, K. K. Niakan, L. M. Weisenthal, H. Mitsumoto, W. Chung, G. F. Croft, G. Saphier, R. Leibel, R. Goland, et al. (2008)
Science 321, 1218-1221
   Abstract »    Full Text »    PDF »
Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling.
D. C. Hay, J. Fletcher, C. Payne, J. D. Terrace, R. C. J. Gallagher, J. Snoeys, J. R. Black, D. Wojtacha, K. Samuel, Z. Hannoun, et al. (2008)
PNAS 105, 12301-12306
   Abstract »    Full Text »    PDF »


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