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Science 23 October 1998:
Vol. 282. no. 5389, pp. 759 - 762
DOI: 10.1126/science.282.5389.759
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Reports

Attenuation of Virulence by Disruption of the Mycobacterium tuberculosis erp Gene

François-Xavier Berthet, * Micheline Lagranderie, Pierre Gounon, Christine Laurent-Winter, Danielle Ensergueix, Pierre Chavarot, Françoise Thouron, Eddie Maranghi, Vladimir Pelicic, dagger Denis Portnoï, Gilles Marchal, Brigitte Gicquel ddagger

The virulence of the mycobacteria that cause tuberculosis depends on their ability to multiply in mammalian hosts. Disruption of the bacterial erp gene, which encodes the exported repetitive protein, impaired multiplication of M. tuberculosis and M. bovis Bacille Calmette-Guérin in cultured macrophages and mice. Reintroduction of erp into the mutants restored their ability to multiply. These results indicate that erp contributes to the virulence of M. tuberculosis.

F.-X. Berthet, D. Ensergueix, V. Pelicic, D. Portnoï, Unité de Génétique Mycobactérienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France. M. Lagranderie and F. Thouron, Laboratoire du BCG, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France. P. Gounon, Station Centrale de Microscopie Electronique, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France. C. Laurent-Winter, Laboratoire d'Electrophorèse Bidimensionnelle, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France. P. Chavarot, E. Maranghi, G. Marchal, Unité de Physiopathologie de l'Infection, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France. B. Gicquel, Unité de Génétique Mycobactérienne, Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France, and Laboratoire du Bacille Calmette Guérin (BCG), Institut Pasteur, 25 rue du Docteur Roux, 75724 Paris Cédex 15, France.
*   Present address: SmithKline Beecham Biologicals, Laboratory of Molecular Bacteriology, Rue de l'Institut 89, B-1330 Rixensart, Belgium.

dagger    Present address: Department of Biological Sciences, University of Padova, Via Trieste 75, 35121 Padova, Italy.

ddagger    To whom correspondence should be addressed. E-mail: bgicquel{at}pasteur.fr

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