Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 16 October 1998:
Vol. 282. no. 5388, pp. 493 - 496
DOI: 10.1126/science.282.5388.493
Prev | Table of Contents

Reports

Two Modes of Survival of Fission Yeast Without Telomerase

Toru M. Nakamura, Julia Promisel Cooper, Thomas R. Cech *

Deletion of the telomerase catalytic subunit gene trt1+ in Schizosaccharomyces pombe results in death for the majority of cells, but a subpopulation survives. Here it is shown that most survivors have circularized all of their chromosomes, whereas a smaller number maintain their telomeres presumably through recombination. When the telomeric DNA-binding gene taz1+ is also deleted, trt1- taz1- survivors use the recombinational mode more frequently. Moreover, the massive elongation of telomeres in taz1- cells is absent in the double mutant. Thus, Taz1p appears to regulate telomeric recombination as well as telomerase activity in fission yeast.

T. M. Nakamura and T. R. Cech, Howard Hughes Medical Institute, Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309-0215, USA. J. P. Cooper, Department of Biochemistry and Molecular Genetics, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
*   To whom correspondence should be addressed.

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Fission Yeast Pot1-Tpp1 Protects Telomeres and Regulates Telomere Length.
T. Miyoshi, J. Kanoh, M. Saito, and F. Ishikawa (2008)
Science 320, 1341-1344
   Abstract »    Full Text »    PDF »
Recombination-Based Telomere Maintenance Is Dependent on Tel1-MRN and Rap1 and Inhibited by Telomerase, Taz1, and Ku in Fission Yeast.
L. Subramanian, B. A. Moser, and T. M. Nakamura (2008)
Mol. Cell. Biol. 28, 1443-1455
   Abstract »    Full Text »    PDF »
Identification and Characterization of an Essential Telomeric Repeat Binding Factor in Fission Yeast.
C. W. Pitt, L. P. Valente, D. Rhodes, and T. Simonsson (2008)
J. Biol. Chem. 283, 2693-2701
   Abstract »    Full Text »    PDF »
The Telotype Defines the Telomere State in Saccharomyces cerevisiae and Is Inherited as a Dominant Non-Mendelian Characteristic in Cells Lacking Telomerase.
S. Makovets, T. L. Williams, and E. H. Blackburn (2008)
Genetics 178, 245-257
   Abstract »    Full Text »    PDF »
Engineered Plant Minichromosomes: A Bottom-Up Success?.
A. Houben, R. K. Dawe, J. Jiang, and I. Schubert (2008)
PLANT CELL 20, 8-10
   Full Text »    PDF »
Fission Yeast Taz1 and RPA Are Synergistically Required to Prevent Rapid Telomere Loss.
T. Kibe, Y. Ono, K. Sato, and M. Ueno (2007)
Mol. Biol. Cell 18, 2378-2387
   Abstract »    Full Text »    PDF »
The Role Of Nonhomologous End-Joining Components in Telomere Metabolism in Kluyveromyces lactis.
S. D. Carter, S. Iyer, J. Xu, M. J. McEachern, and S. U. Astrom (2007)
Genetics 175, 1035-1045
   Abstract »    Full Text »    PDF »
Role of SUMO in the dynamics of telomere maintenance in fission yeast.
B. Xhemalce, E. M. Riising, P. Baumann, A. Dejean, B. Arcangioli, and J.-S. Seeler (2007)
PNAS 104, 893-898
   Abstract »    Full Text »    PDF »
Telomerase-Independent Stabilization of Short Telomeres in Trypanosoma brucei..
O. Dreesen and G. A. M. Cross (2006)
Mol. Cell. Biol. 26, 4911-4919
   Abstract »    Full Text »    PDF »
Evolutionary-conserved telomere-linked helicase genes of fission yeast are repressed by silencing factors, RNAi components and the telomere-binding protein Taz1.
K. R. Hansen, P. T. Ibarra, and G. Thon (2006)
Nucleic Acids Res. 34, 78-88
   Abstract »    Full Text »    PDF »
Complete DNA sequences of the mitochondrial genomes of the pathogenic yeasts Candida orthopsilosis and Candida metapsilosis: insight into the evolution of linear DNA genomes from mitochondrial telomere mutants..
P. Kosa, M. Valach, L. Tomaska, K. H. Wolfe, and J. Nosek (2006)
Nucleic Acids Res. 34, 2472-2481
   Abstract »    Full Text »    PDF »
Distinct Requirements for Pot1 in Limiting Telomere Length and Maintaining Chromosome Stability.
J. T. Bunch, N. S. Bae, J. Leonardi, and P. Baumann (2005)
Mol. Cell. Biol. 25, 5567-5578
   Abstract »    Full Text »    PDF »
Extended DNA Binding Site in Pot1 Broadens Sequence Specificity to Allow Recognition of Heterogeneous Fission Yeast Telomeres.
K. M. Trujillo, J. T. Bunch, and P. Baumann (2005)
J. Biol. Chem. 280, 9119-9128
   Abstract »    Full Text »    PDF »
The unusually large Plasmodium telomerase reverse-transcriptase localizes in a discrete compartment associated with the nucleolus.
L. M. Figueiredo, E. P. C. Rocha, L. Mancio-Silva, C. Prevost, D.čl. Hernandez-Verdun, and A. Scherf (2005)
Nucleic Acids Res. 33, 1111-1122
   Abstract »    Full Text »    PDF »
Expression of a RecQ Helicase Homolog Affects Progression through Crisis in Fission Yeast Lacking Telomerase.
J. G. Mandell, K. J. Goodrich, J. Bahler, and T. R. Cech (2005)
J. Biol. Chem. 280, 5249-5257
   Abstract »    Full Text »    PDF »
Ty1 Mobilizes Subtelomeric Y' Elements in Telomerase-Negative Saccharomyces cerevisiae Survivors.
P. H. Maxwell, C. Coombes, A. E. Kenny, J. F. Lawler, J. D. Boeke, and M. J. Curcio (2004)
Mol. Cell. Biol. 24, 9887-9898
   Abstract »    Full Text »    PDF »
Telomerase- and recombination-independent immortalization of budding yeast.
L. Maringele and D. Lydall (2004)
Genes & Dev. 18, 2663-2675
   Abstract »    Full Text »    PDF »
Two modes of DNA double-strand break repair are reciprocally regulated through the fission yeast cell cycle.
M. G. Ferreira and J. P. Cooper (2004)
Genes & Dev. 18, 2249-2254
   Abstract »    Full Text »    PDF »
Functional links between telomeres and proteins of the DNA-damage response.
F. d'Adda di Fagagna, S.-H. Teo, and S. P. Jackson (2004)
Genes & Dev. 18, 1781-1799
   Abstract »    Full Text »    PDF »
Linear versus circular mitochondrial genomes: intraspecies variability of mitochondrial genome architecture in Candida parapsilosis.
A. Rycovska, M. Valach, L. Tomaska, M. Bolotin-Fukuhara, and J. Nosek (2004)
Microbiology 150, 1571-1580
   Abstract »    Full Text »    PDF »
End Resection Initiates Genomic Instability in the Absence of Telomerase.
J. A. Hackett and C. W. Greider (2003)
Mol. Cell. Biol. 23, 8450-8461
   Abstract »    Full Text »    PDF »
The Fission Yeast Rad32 (Mre11)-Rad50-Nbs1 Complex Is Required for the S-Phase DNA Damage Checkpoint.
C. Chahwan, T. M. Nakamura, S. Sivakumar, P. Russell, and N. Rhind (2003)
Mol. Cell. Biol. 23, 6564-6573
   Abstract »    Full Text »    PDF »
Stable inheritance of telomere chromatin structure and function in the absence of telomeric repeats.
M. Sadaie, T. Naito, and F. Ishikawa (2003)
Genes & Dev. 17, 2271-2282
   Abstract »    Full Text »    PDF »
Fission yeast Rhp51 is required for the maintenance of telomere structure in the absence of the Ku heterodimer.
T. Kibe, K. Tomita, A. Matsuura, D. Izawa, T. Kodaira, T. Ushimaru, M. Uritani, and M. Ueno (2003)
Nucleic Acids Res. 31, 5054-5063
   Abstract »    Full Text »    PDF »
Competition between the Rad50 Complex and the Ku Heterodimer Reveals a Role for Exo1 in Processing Double-Strand Breaks but Not Telomeres.
K. Tomita, A. Matsuura, T. Caspari, A. M. Carr, Y. Akamatsu, H. Iwasaki, K.-i. Mizuno, K. Ohta, M. Uritani, T. Ushimaru, et al. (2003)
Mol. Cell. Biol. 23, 5186-5197
   Abstract »    Full Text »    PDF »
Telomerase-Independent Proliferation Is Influenced by Cell Type in Saccharomyces cerevisiae.
J. E. Lowell, A. I. Roughton, V. Lundblad, and L. Pillus (2003)
Genetics 164, 909-921
   Abstract »    Full Text »    PDF »
Replication Proteins Influence the Maintenance of Telomere Length and Telomerase Protein Stability.
M. Dahlen, P. Sunnerhagen, and T. S.-F. Wang (2003)
Mol. Cell. Biol. 23, 3031-3042
   Abstract »    Full Text »    PDF »
Telomere Dynamics in Myelodysplastic Syndrome Determined by Telomere Measurement of Marrow Metaphases.
G. Sashida, J. H. Ohyashiki, A. Nakajima, M. Sumi, K. Kawakubo, T. Tauchi, and K. Ohyashiki (2003)
Clin. Cancer Res. 9, 1489-1496
   Abstract »    Full Text »    PDF »
Telomerase Dependence of Telomere Lengthening in ku80 Mutant Arabidopsis.
M. E. Gallego, N. Jalut, and C. I. White (2003)
PLANT CELL 15, 782-789
   Abstract »    Full Text »    PDF »
Short Telomeres Induce a DNA Damage Response in Saccharomyces cerevisiae.
A. S. IJpma and C. W. Greider (2003)
Mol. Biol. Cell 14, 987-1001
   Abstract »    Full Text »    PDF »
Telomeric DNA Ends Are Essential for the Localization of Ku at Telomeres in Fission Yeast.
T. Miyoshi, M. Sadaie, J. Kanoh, and F. Ishikawa (2003)
J. Biol. Chem. 278, 1924-1931
   Abstract »    Full Text »    PDF »
Telomere Binding of Checkpoint Sensor and DNA Repair Proteins Contributes to Maintenance of Functional Fission Yeast Telomeres.
T. M. Nakamura, B. A. Moser, and P. Russell (2002)
Genetics 161, 1437-1452
   Abstract »    Full Text »    PDF »
Recombinational Telomere Elongation Promoted by DNA Circles.
S. Natarajan and M. J. McEachern (2002)
Mol. Cell. Biol. 22, 4512-4521
   Abstract »    Full Text »    PDF »
A Quantitative Assay for Telomere Protection in Saccharomyces cerevisiae.
M. L. DuBois, Z. W. Haimberger, M. W. McIntosh, and D. E. Gottschling (2002)
Genetics 161, 995-1013
   Abstract »    Full Text »    PDF »
Schizosaccharomyces pombe pfh1+ Encodes an Essential 5' to 3' DNA Helicase That Is a Member of the PIF1 Subfamily of DNA Helicases.
J.-Q. Zhou, H. Qi, V. P. Schulz, M. K. Mateyak, E. K. Monson, and V. A. Zakian (2002)
Mol. Biol. Cell 13, 2180-2191
   Abstract »    Full Text »    PDF »
Transcriptional silencing in Saccharomyces cerevisiae and Schizosaccharomyces pombe.
Y. Huang (2002)
Nucleic Acids Res. 30, 1465-1482
   Abstract »    Full Text »    PDF »
How do tumors make ends meet?.
C. Lengauer (2001)
PNAS 98, 12331-12333
   Full Text »    PDF »
Pot1, the Putative Telomere End-Binding Protein in Fission Yeast and Humans.
P. Baumann and T. R. Cech (2001)
Science 292, 1171-1175
   Abstract »    Full Text »
Extragenomic double-stranded DNA circles in yeast with linear mitochondrial genomes: potential involvement in telomere maintenance.
L. Tomaska, J. Nosek, A. M. Makhov, A. Pastorakova, and J. D. Griffith (2000)
Nucleic Acids Res. 28, 4479-4487
   Abstract »    Full Text »    PDF »
Aging mechanisms.
Y. Takahashi, M. Kuro-o, and F. Ishikawa (2000)
PNAS
   Abstract »    Full Text »
Protection of Telomeres by the Ku Protein in Fission Yeast.
P. Baumann and T. R. Cech (2000)
Mol. Biol. Cell 11, 3265-3275
   Abstract »    Full Text »
Telomere fusions caused by mutating the terminal region of telomeric DNA.
M. J. McEachern, S. Iyer, T. B. Fulton, and E. H. Blackburn (2000)
PNAS
   Abstract »    Full Text »
Role of Telomerase in Normal and Cancer Cells.
M. Meyerson (2000)
J. Clin. Oncol. 18, 2626-2634
   Abstract »    Full Text »    PDF »
Telomere Maintenance in Telomerase-Deficient Mouse Embryonic Stem Cells: Characterization of an Amplified Telomeric DNA.
H. Niida, Y. Shinkai, M. P. Hande, T. Matsumoto, S. Takehara, M. Tachibana, M. Oshimura, P. M. Lansdorp, and Y. Furuichi (2000)
Mol. Cell. Biol. 20, 4115-4127
   Abstract »    Full Text »
Positive and negative regulation of telomerase access to the telomere.
S. Evans and V Lundblad (2000)
J. Cell Sci. 113, 3357-3364
   Abstract »    PDF »
Disruption of the telomerase catalytic subunit gene from Arabidopsis inactivates telomerase and leads to a slow loss of telomeric DNA.
M. S. Fitzgerald, K. Riha, F. Gao, S. Ren, T. D. McKnight, and D. E. Shippen (1999)
PNAS 96, 14813-14818
   Abstract »    Full Text »    PDF »
Telomere-Telomere Recombination Is an Efficient Bypass Pathway for Telomere Maintenance in Saccharomyces cerevisiae.
S.-C. Teng and V. A. Zakian (1999)
Mol. Cell. Biol. 19, 8083-8093
   Abstract »    Full Text »    PDF »
FJ5002: A Potent Telomerase Inhibitor Identified by Exploiting the Disease-oriented Screening Program with COMPARE Analysis.
I. Naasani, H. Seimiya, T. Yamori, and T. Tsuruo (1999)
Cancer Res. 59, 4004-4011
   Abstract »    Full Text »    PDF »
Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae.
F. Paques and J. E. Haber (1999)
Microbiol. Mol. Biol. Rev. 63, 349-404
   Abstract »    Full Text »    PDF »
Telomere Length Dynamics and Chromosomal Instability in Cells Derived from Telomerase Null Mice.
M. P. Hande, E. Samper, P. Lansdorp, and M. A. Blasco (1999)
J. Cell Biol. 144, 589-601
   Abstract »    Full Text »    PDF »
A Plant Gene Encoding a Myb-like Protein That Binds Telomeric GGTTTAG Repeats in Vitro.
C. M. Chen, C. T. Wang, and C. H. Ho (2001)
J. Biol. Chem. 276, 16511-16519
   Abstract »    Full Text »    PDF »
Analysis of telomerase catalytic subunit mutants in vivo and in vitro in Schizosaccharomycespombe.
C. H. Haering, T. M. Nakamura, P. Baumann, and T. R. Cech (2000)
PNAS 97, 6367-6372
   Abstract »    Full Text »    PDF »
Aging mechanisms.
Y. Takahashi, M. Kuro-o, and F. Ishikawa (2000)
PNAS 97, 12407-12408
   Abstract »    Full Text »    PDF »
Telomere fusions caused by mutating the terminal region of telomeric DNA.
M. J. McEachern, S. Iyer, T. B. Fulton, and E. H. Blackburn (2000)
PNAS 97, 11409-11414
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)