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Science 16 October 1998:
Vol. 282. no. 5388, pp. 484 - 487
DOI: 10.1126/science.282.5388.484
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Reports

An Integrated Nanoliter DNA Analysis Device

Mark A. Burns, Brian N. Johnson, Sundaresh N. Brahmasandra, Kalyan Handique, James R. Webster, Madhavi Krishnan, Timothy S. Sammarco, Piu M. Man, Darren Jones, Dylan Heldsinger, Carlos H. Mastrangelo, David T. Burke

A device was developed that uses microfabricated fluidic channels, heaters, temperature sensors, and fluorescence detectors to analyze nanoliter-size DNA samples. The device is capable of measuring aqueous reagent and DNA-containing solutions, mixing the solutions together, amplifying or digesting the DNA to form discrete products, and separating and detecting those products. No external lenses, heaters, or mechanical pumps are necessary for complete sample processing and analysis. Because all of the components are made using conventional photolithographic production techniques, they operate as a single closed system. The components have the potential for assembly into complex, low-power, integrated analysis systems at low unit cost. The availability of portable, reliable instruments may facilitate the use of DNA analysis in applications such as rapid medical diagnostics and point-of-use agricultural testing.

M. A. Burns, B. N. Johnson, S. N. Brahmasandra, K. Handique, T. S. Sammarco, D. Heldsinger, Department of Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. M. Krishnan, Department of Biomedical Engineering and Chemical Engineering, University of Michigan, Ann Arbor, MI 48109, USA. J. R. Webster, P. M. Man, D. Jones, C. H. Mastrangelo, Department of Electrical Engineering and Computer Science, University of Michigan, Ann Arbor, MI 48109, USA. D. T. Burke, Department of Human Genetics, University of Michigan, Ann Arbor, MI 48109, USA.

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