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Science 9 October 1998:
Vol. 282. no. 5387, pp. 281 - 284
DOI: 10.1126/science.282.5387.281
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Reports

Isolation of Acidophilic Methane-Oxidizing Bacteria from Northern Peat Wetlands

Svetlana N. Dedysh, Nicolai S. Panikov, Werner Liesack, Regine Großkopf, Jizhong Zhou, James M. Tiedje *

Acidic northern wetlands are an important source of methane, one of the gases that contributes to global warming. Methane oxidation in the surface of these acidic wetlands can reduce the methane flux to the atmosphere up to 90 percent. Here the isolation of three methanotrophic microorganisms from three boreal forest sites is reported. They are moderately acidophilic organisms and have a soluble methane monooxygenase. In contrast to the known groups of methanotrophs, 16S ribosomal DNA sequence analysis shows that they are affiliated with the acidophilic heterotrophic bacterium Beijerinckia indica subsp. indica.

S. N. Dedysh, Institute of Microbiology, Russian Academy of Sciences, Moscow 117811, Russia, and Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824-1325, USA. N. S. Panikov, Institute of Microbiology, Russian Academy of Sciences, Moscow 117811, Russia. W. Liesack and R. Großkopf, Max-Planck-Institut für Terrestrische Mikrobiologie, D-35043 Marburg, Germany. J. Zhou and J. M. Tiedje, Center for Microbial Ecology, Michigan State University, East Lansing, MI 48824-1325, USA.
*   To whom correspondence should be addressed. E-mail: tiedjej{at}pilot.msu.edu

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THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Buoyancy-driven flow in a peat moss layer as a mechanism for solute transport.
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Detection and Enumeration of Methanotrophs in Acidic Sphagnum Peat by 16S rRNA Fluorescence In Situ Hybridization, Including the Use of Newly Developed Oligonucleotide Probes for Methylocella palustris.
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Molecular Analyses of the Methane-Oxidizing Microbial Community in Rice Field Soil by Targeting the Genes of the 16S rRNA, Particulate Methane Monooxygenase, and Methanol Dehydrogenase.
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