Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 2 October 1998:
Vol. 282. no. 5386, pp. 100 - 103
DOI: 10.1126/science.282.5386.100
Prev | Table of Contents | Next

Reports

An Arabidopsis Mutant Defective in the Plastid General Protein Import Apparatus

Paul Jarvis, Lih-Jen Chen, Hsou-min Li, Charles A. Peto, Christian Fankhauser, Joanne Chory *

Elaborate mechanisms have evolved for the translocation of nucleus-encoded proteins across the plastid envelope membrane. Although putative components of the import apparatus have been identified biochemically, their role in import remains to be proven in vivo. An Arabidopsis mutant lacking a new component of the import machinery [translocon at the outer envelope membrane of chloroplasts (Toc33), a 33-kilodalton protein] has been isolated. The functional similarity of Toc33 to another translocon component (Toc34) implies that multiple different translocon complexes are present in plastids. Processes that are mediated by Toc33 operate during the early stages of plastid and leaf development. The data demonstrate the in vivo role of a translocon component in plastid protein import.

P. Jarvis and C. Fankhauser, Plant Biology Laboratory, Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. L.-J. Chen and H.-m. Li, Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan. C. A. Peto, Laboratory of Neuronal Structure and Function, Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA. J. Chory, Plant Biology Laboratory and Howard Hughes Medical Institute, Salk Institute, 10010 North Torrey Pines Road, La Jolla, CA 92037, USA.
*   To whom correspondence should be addressed. E-mail: chory{at}salk.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
CIA2 Coordinately Up-Regulates Protein Import and Synthesis in Leaf Chloroplasts.
C.-W. Sun, Y.-C. Huang, and H.-Y. Chang (2009)
Plant Physiology 150, 879-888
   Abstract »    Full Text »    PDF »
A Toc159 Import Receptor Mutant, Defective in Hydrolysis of GTP, Supports Preprotein Import into Chloroplasts.
B. Agne, S. Infanger, F. Wang, V. Hofstetter, G. Rahim, M. Martin, D. W. Lee, I. Hwang, D. Schnell, and F. Kessler (2009)
J. Biol. Chem. 284, 8670-8679
   Abstract »    Full Text »    PDF »
The role of GTP binding and hydrolysis at the atToc159 preprotein receptor during protein import into chloroplasts.
F. Wang, B. Agne, F. Kessler, and D. J. Schnell (2008)
J. Cell Biol. 183, 87-99
   Abstract »    Full Text »    PDF »
On the Significance of Toc-GTPase Homodimers.
P. Koenig, M. Oreb, K. Rippe, C. Muhle-Goll, I. Sinning, E. Schleiff, and I. Tews (2008)
J. Biol. Chem. 283, 23104-23112
   Abstract »    Full Text »    PDF »
A 125 kDa RNase E/G-like protein is present in plastids and is essential for chloroplast development and autotrophic growth in Arabidopsis.
E. A. Mudd, S. Sullivan, M. F. Gisby, A. Mironov, C. S. Kwon, W.-I. Chung, and A. Day (2008)
J. Exp. Bot. 59, 2597-2610
   Abstract »    Full Text »    PDF »
Phosphorylation regulates the assembly of chloroplast import machinery.
M. Oreb, A. Hofle, O. Mirus, and E. Schleiff (2008)
J. Exp. Bot. 59, 2309-2316
   Abstract »    Full Text »    PDF »
Comparative Analysis of the MIR319a MicroRNA Locus in Arabidopsis and Related Brassicaceae.
N. Warthmann, S. Das, C. Lanz, and D. Weigel (2008)
Mol. Biol. Evol. 25, 892-902
   Abstract »    Full Text »    PDF »
Arabidopsis CAPRICE-LIKE MYB 3 (CPL3) controls endoreduplication and flowering development in addition to trichome and root hair formation.
R. Tominaga, M. Iwata, R. Sano, K. Inoue, K. Okada, and T. Wada (2008)
Development 135, 1335-1345
   Abstract »    Full Text »    PDF »
Arabidopsis Stromal 70-kD Heat Shock Proteins Are Essential for Plant Development and Important for Thermotolerance of Germinating Seeds.
P.-H. Su and H.-m. Li (2008)
Plant Physiology 146, 1231-1241
   Abstract »    Full Text »    PDF »
A substrate-independent, 14:3:3 protein-mediated plastid import pathway of NADPH:protochlorophyllide oxidoreductase A.
A. Schemenewitz, S. Pollmann, C. Reinbothe, and S. Reinbothe (2007)
PNAS 104, 8538-8543
   Abstract »    Full Text »    PDF »
Dimerization Is Important for the GTPase Activity of Chloroplast Translocon Components atToc33 and psToc159.
Y.-H. Yeh, M. M. Kesavulu, H.-m. Li, S.-Z. Wu, Y.-J. Sun, E. H. E. Konozy, and C.-D. Hsiao (2007)
J. Biol. Chem. 282, 13845-13853
   Abstract »    Full Text »    PDF »
Tic21 Is an Essential Translocon Component for Protein Translocation across the Chloroplast Inner Envelope Membrane.
Y.-S. Teng, Y.-s. Su, L.-J. Chen, Y. J. Lee, I. Hwang, and H.-m. Li (2006)
PLANT CELL 18, 2247-2257
   Abstract »    Full Text »    PDF »
A role for chlorophyllide a oxygenase in the regulated import and stabilization of light-harvesting chlorophyll a/b proteins.
C. Reinbothe, S. Bartsch, L. L. Eggink, J. K. Hoober, J. Brusslan, R. Andrade-Paz, J. Monnet, and S. Reinbothe (2006)
PNAS 103, 4777-4782
   Abstract »    Full Text »    PDF »
Recognition and envelope translocation of chloroplast preproteins.
J. Bedard and P. Jarvis (2005)
J. Exp. Bot. 56, 2287-2320
   Abstract »    Full Text »    PDF »
A Molecular-Genetic Study of the Arabidopsis Toc75 Gene Family.
A. Baldwin, A. Wardle, R. Patel, P. Dudley, S. K. Park, D. Twell, K. Inoue, and P. Jarvis (2005)
Plant Physiology 138, 715-733
   Abstract »    Full Text »    PDF »
Evidence That the Plastid Translocon Tic40 Components Possess Modulating Capabilities.
K. Ko, D. Taylor, P. Argenton, J. Innes, B. Pedram, F. Seibert, A. Granell, and Z. Ko (2005)
J. Biol. Chem. 280, 215-224
   Abstract »    Full Text »    PDF »
Deletion of the Chloroplast-Localized Thylakoid Formation1 Gene Product in Arabidopsis Leads to Deficient Thylakoid Formation and Variegated Leaves.
Q. Wang, R. W. Sullivan, A. Kight, R. L. Henry, J. Huang, A. M. Jones, and K. L. Korth (2004)
Plant Physiology 136, 3594-3604
   Abstract »    Full Text »    PDF »
Mutations in ClpC2/Hsp100 suppress the requirement for FtsH in thylakoid membrane biogenesis.
S. Park and S. R. Rodermel (2004)
PNAS 101, 12765-12770
   Abstract »    Full Text »    PDF »
Import Pathways of Chloroplast Interior Proteins and the Outer-Membrane Protein OEP14 Converge at Toc75.
S.-L. Tu, L.-J. Chen, M. D. Smith, Y.-s. Su, D. J. Schnell, and H.-m. Li (2004)
PLANT CELL 16, 2078-2088
   Abstract »    Full Text »    PDF »
Functional Specialization amongst the Arabidopsis Toc159 Family of Chloroplast Protein Import Receptors.
S. Kubis, R. Patel, J. Combe, J. Bedard, S. Kovacheva, K. Lilley, A. Biehl, D. Leister, G. Rios, C. Koncz, et al. (2004)
PLANT CELL 16, 2059-2077
   Abstract »    Full Text »    PDF »
Members of the Toc159 Import Receptor Family Represent Distinct Pathways for Protein Targeting to Plastids.
Y. Ivanova, M. D. Smith, K. Chen, and D. J. Schnell (2004)
Mol. Biol. Cell 15, 3379-3392
   Abstract »    Full Text »    PDF »
Identification of plastid envelope proteins required for import of protochlorophyllide oxidoreductase A into the chloroplast of barley.
S. Reinbothe, F. Quigley, J. Gray, A. Schemenewitz, and C. Reinbothe (2004)
PNAS 101, 2197-2202
   Abstract »    Full Text »    PDF »
The outer plastid envelope protein Oep16: Role as precursor translocase in import of protochlorophyllide oxidoreductase A.
S. Reinbothe, F. Quigley, A. Springer, A. Schemenewitz, and C. Reinbothe (2004)
PNAS 101, 2203-2208
   Abstract »    Full Text »    PDF »
Substrate-Dependent and Organ-Specific Chloroplast Protein Import in Planta.
C. Kim and K. Apel (2004)
PLANT CELL 16, 88-98
   Abstract »    Full Text »    PDF »
The Roles of Toc34 and Toc75 in Targeting the Toc159 Preprotein Receptor to Chloroplasts.
T. R. Wallas, M. D. Smith, S. Sanchez-Nieto, and D. J. Schnell (2003)
J. Biol. Chem. 278, 44289-44297
   Abstract »    Full Text »    PDF »
The M Domain of atToc159 Plays an Essential Role in the Import of Proteins into Chloroplasts and Chloroplast Biogenesis.
K. H. Lee, S. J. Kim, Y. J. Lee, J. B. Jin, and I. Hwang (2003)
J. Biol. Chem. 278, 36794-36805
   Abstract »    Full Text »    PDF »
The Arabidopsis ppi1 Mutant Is Specifically Defective in the Expression, Chloroplast Import, and Accumulation of Photosynthetic Proteins.
S. Kubis, A. Baldwin, R. Patel, A. Razzaq, P. Dupree, K. Lilley, J. Kurth, D. Leister, and P. Jarvis (2003)
PLANT CELL 15, 1859-1871
   Abstract »    Full Text »    PDF »
A GTP-driven motor moves proteins across the outer envelope of chloroplasts.
E. Schleiff, M. Jelic, and J. Soll (2003)
PNAS 100, 4604-4609
   Abstract »    Full Text »    PDF »
Characterization of the translocon of the outer envelope of chloroplasts.
E. Schleiff, J. Soll, M. Kuchler, W. Kuhlbrandt, and R. Harrer (2003)
J. Cell Biol. 160, 541-551
   Abstract »    Full Text »    PDF »
The Arabidopsis SRR1 gene mediates phyB signaling and is required for normal circadian clock function.
D. Staiger, L. Allenbach, N. Salathia, V. Fiechter, S. J. Davis, A. J. Millar, J. Chory, and C. Fankhauser (2003)
Genes & Dev. 17, 256-268
   Abstract »    Full Text »    PDF »
Essential role of the G-domain in targeting of the protein import receptor atToc159 to the chloroplast outer membrane.
J. Bauer, A. Hiltbrunner, P. Weibel, P.-A. Vidi, M. Alvarez-Huerta, M. D. Smith, D. J. Schnell, and F. Kessler (2002)
J. Cell Biol. 159, 845-854
   Abstract »    Full Text »    PDF »
The targeting of the atToc159 preprotein receptor to the chloroplast outer membrane is mediated by its GTPase domain and is regulated by GTP.
M. D. Smith, A. Hiltbrunner, F. Kessler, and D. J. Schnell (2002)
J. Cell Biol. 159, 833-843
   Abstract »    Full Text »    PDF »
Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning.
G. J. Budziszewski, S. P. Lewis, L. W. Glover, J. Reineke, G. Jones, L. S. Ziemnik, J. Lonowski, B. Nyfeler, G. Aux, Q. Zhou, et al. (2001)
Genetics 159, 1765-1778
   Abstract »    Full Text »    PDF »
Leaf-Specific Upregulation of Chloroplast Translocon Genes by a CCT Motif-Containing Protein, CIA 2.
C.-W. Sun, L.-J. Chen, L.-C. Lin, and H.-m. Li (2001)
PLANT CELL 13, 2053-2061
   Abstract »    Full Text »    PDF »
Chloroplast Protein Translocon Components atToc159 and atToc33 Are Not Essential for Chloroplast Biogenesis in Guard Cells and Root Cells.
T.-S. Yu and H.-m. Li (2001)
Plant Physiology 127, 90-96
   Abstract »    Full Text »    PDF »
An essential role of a TatC homologue of a Delta pH- dependent protein transporter in thylakoid membrane formation during chloroplast development in Arabidopsis thaliana.
R. Motohashi, N. Nagata, T. Ito, S. Takahashi, T. Hobo, S. Yoshida, and K. Shinozaki (2001)
PNAS 98, 10499-10504
   Abstract »    Full Text »    PDF »
Targeting of an abundant cytosolic form of the protein import receptor at Toc159 to the outer chloroplast membrane.
A. Hiltbrunner, J. Bauer, P.-A. Vidi, S. Infanger, P. Weibel, M. Hohwy, and F. Kessler (2001)
J. Cell Biol. 154, 309-316
   Abstract »    Full Text »    PDF »
shygrl1 Is a Mutant Affected in Multiple Aspects of Photomorphogenesis.
M. Santiago-Ong, R. M. Green, S. Tingay, J. A. Brusslan, and E. M. Tobin (2001)
Plant Physiology 126, 587-600
   Abstract »    Full Text »    PDF »
Arabidopsis Genes Encoding Components of the Chloroplastic Protein Import Apparatus.
D. Jackson-Constan and K. Keegstra (2001)
Plant Physiology 125, 1567-1576
   Abstract »    Full Text »
Maize Non-Photosynthetic Ferredoxin Precursor Is Mis-Sorted to the Intermembrane Space of Chloroplasts in the Presence of Light.
T. Hirohashi, T. Hase, and M. Nakai (2001)
Plant Physiology 125, 2154-2163
   Abstract »    Full Text »
Chloroplast precursor proteins compete to form early import intermediates in isolated pea chloroplasts.
P. E. Row and J. C. Gray (2001)
J. Exp. Bot. 52, 47-56
   Abstract »    Full Text »    PDF »
Trienoic Fatty Acids Are Required to Maintain Chloroplast Function at Low Temperatures.
J.-M. Routaboul, S. F. Fischer, and J. Browse (2000)
Plant Physiology 124, 1697-1705
   Abstract »    Full Text »
A second, substrate-dependent site of protein import into chloroplasts.
S. Reinbothe, R. Mache, and C. Reinbothe (2000)
PNAS
   Abstract »    Full Text »
Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant.
P. Jarvis, P. Dörmann, C. A. Peto, J. Lutes, C. Benning, and J. Chory (2000)
PNAS
   Abstract »    Full Text »
Toc64, a New Component of the Protein Translocon of Chloroplasts.
K. Sohrt and J. Soll (2000)
J. Cell Biol. 148, 1213-1222
   Abstract »    Full Text »    PDF »
GTP Promotes the Formation of Early-Import Intermediates But Is Not Required during the Translocation Step of Protein Import into Chloroplasts.
M. E. Young, K. Keegstra, and J. E. Froehlich (1999)
Plant Physiology 121, 237-244
   Abstract »    Full Text »
Insertion of atToc34 into the Chloroplastic Outer Membrane Is Assisted by at Least Two Proteinaceous Components in the Import System.
L.-Y. Tsai, S.-L. Tu, and H.-m. Li (1999)
J. Biol. Chem. 274, 18735-18740
   Abstract »    Full Text »    PDF »
Protein Import and Routing Systems of Chloroplasts.
K. Keegstra and K. Cline (1999)
PLANT CELL 11, 557-570
   Full Text »
Galactolipid deficiency and abnormal chloroplast development in the Arabidopsis MGD synthase 1 mutant.
P. Jarvis, P. Dormann, C. A. Peto, J. Lutes, C. Benning, and J. Chory (2000)
PNAS 97, 8175-8179
   Abstract »    Full Text »    PDF »
A second, substrate-dependent site of protein import into chloroplasts.
S. Reinbothe, R. Mache, and C. Reinbothe (2000)
PNAS 97, 9795-9800
   Abstract »    Full Text »    PDF »
In Vivo Analysis of the Role of atTic20 in Protein Import into Chloroplasts.
X. Chen, M. D. Smith, L. Fitzpatrick, and D. J. Schnell (2002)
PLANT CELL 14, 641-654
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)