Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Invitrogen

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 25 September 1998:
Vol. 281. no. 5385, pp. 2016 - 2018
DOI: 10.1126/science.281.5385.2016
Prev | Table of Contents | Next

Reports

Quantum Dot Bioconjugates for Ultrasensitive Nonisotopic Detection

Warren C. W. Chan, Shuming Nie *

Highly luminescent semiconductor quantum dots (zinc sulfide-capped cadmium selenide) have been covalently coupled to biomolecules for use in ultrasensitive biological detection. In comparison with organic dyes such as rhodamine, this class of luminescent labels is 20 times as bright, 100 times as stable against photobleaching, and one-third as wide in spectral linewidth. These nanometer-sized conjugates are water-soluble and biocompatible. Quantum dots that were labeled with the protein transferrin underwent receptor-mediated endocytosis in cultured HeLa cells, and those dots that were labeled with immunomolecules recognized specific antibodies or antigens.

Department of Chemistry, Indiana University, Bloomington, IN 47405, USA.
*   To whom correspondence should be addressed. E-mail: nie{at}indiana.edu

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nanoparticles as Fluorescence Labels: Is Size All that Matters?.
J. L. Swift and D. T. Cramb (2008)
Biophys. J. 95, 865-876
   Abstract »    Full Text »    PDF »
Characterization of Water-soluble Luminescent Quantum Dots by Fluorescence Correlation Spectroscopy.
C. DONG, X. HUANG, and J. REN (2008)
Ann. N.Y. Acad. Sci. 1130, 253-261
   Abstract »    Full Text »    PDF »
NSOM/QD-based nanoscale immunofluorescence imaging of antigen-specific T-cell receptor responses during an in vivo clonal V{gamma}2V{delta}2 T-cell expansion.
Y. Chen, L. Shao, Z. Ali, J. Cai, and Z. W. Chen (2008)
Blood 111, 4220-4232
   Abstract »    Full Text »    PDF »
Nanometer-scale mapping and single-molecule detection with color-coded nanoparticle probes.
A. Agrawal, R. Deo, G. D. Wang, M. D. Wang, and S. Nie (2008)
PNAS 105, 3298-3303
   Abstract »    Full Text »    PDF »
Application of Nanotechnology in Cancer Therapy and Imaging.
X. Wang, L. Yang, Z. Chen, and D. M. Shin (2008)
CA Cancer J Clin 58, 97-110
   Abstract »    Full Text »    PDF »
The Application of Fluorescent Quantum Dots to Confocal, Multiphoton, and Electron Microscopic Imaging.
T. J. Deerinck (2008)
Toxicol Pathol 36, 112-116
   Abstract »    Full Text »    PDF »
Electron microscopic analysis of a fusion protein of postsynaptic density-95 and metallothionein in cultured hippocampal neurons.
Y. Fukunaga, A. Hirase, H. Kim, N. Wada, Y. Nishino, and A. Miyazawa (2007)
J. Electron Microsc. (Tokyo)
   Abstract »    Full Text »    PDF »
Evidence for a diffusion-controlled mechanism for fluorescence blinking of colloidal quantum dots.
M. Pelton, G. Smith, N. F. Scherer, and R. A. Marcus (2007)
PNAS 104, 14249-14254
   Abstract »    Full Text »    PDF »
Quantum Dots: A Quantum Jump for Molecular Imaging?.
K.-H. Lee (2007)
J. Nucl. Med. 48, 1408-1410
   Full Text »    PDF »
Detection and Correction of Blinking Bias in Image Correlation Transport Measurements of Quantum Dot Tagged Macromolecules.
N. Durisic, A. I. Bachir, D. L. Kolin, B. Hebert, B. C. Lagerholm, P. Grutter, and P. W. Wiseman (2007)
Biophys. J. 93, 1338-1346
   Abstract »    Full Text »    PDF »
Finding Fluorescent Needles in the Cardiac Haystack: Tracking Human Mesenchymal Stem Cells Labeled with Quantum Dots for Quantitative In Vivo Three-Dimensional Fluorescence Analysis.
A. B. Rosen, D. J. Kelly, A. J. T. Schuldt, J. Lu, I. A. Potapova, S. V. Doronin, K. J. Robichaud, R. B. Robinson, M. R. Rosen, P. R. Brink, et al. (2007)
Stem Cells 25, 2128-2138
   Abstract »    Full Text »    PDF »
Single-molecule Chemistry and Biology Special Feature: Strongly emissive individual DNA-encapsulated Ag nanoclusters as single-molecule fluorophores.
T. Vosch, Y. Antoku, J.-C. Hsiang, C. I. Richards, J. I. Gonzalez, and R. M. Dickson (2007)
PNAS 104, 12616-12621
   Abstract »    Full Text »    PDF »
Spontaneous Superlattice Formation in Nanorods Through Partial Cation Exchange.
R. D. Robinson, B. Sadtler, D. O. Demchenko, C. K. Erdonmez, L.-W. Wang, and A. P. Alivisatos (2007)
Science 317, 355-358
   Abstract »    Full Text »    PDF »
Parsing the Motion of Single Molecules: A Novel Algorithm for Deconvoluting the Dynamics of Individual Receptors at the Cell Surface.
I. Ghosh and M. J. Wirth (2007)
Sci. STKE 2007, pe28
   Abstract »    Full Text »    PDF »
Semiautomated Multiplexed Quantum Dot-Based in Situ Hybridization and Spectral Deconvolution.
R. J. Byers, D. Di Vizio, F. O'Connell, E. Tholouli, R. M. Levenson, K. Gossard, D. Twomey, Y. Yang, E. Benedettini, J. Rose, et al. (2007)
J. Mol. Diagn. 9, 20-29
   Abstract »    Full Text »    PDF »
Use of Quantum Dot Luminescent Probes To Achieve Single-Cell Resolution of Human Oral Bacteria in Biofilms.
N. I. Chalmers, R. J. Palmer Jr., L. Du-Thumm, R. Sullivan, W. Shi, and P. E. Kolenbrander (2007)
Appl. Envir. Microbiol. 73, 630-636
   Abstract »    Full Text »    PDF »
k-Space Image Correlation Spectroscopy: A Method for Accurate Transport Measurements Independent of Fluorophore Photophysics.
D. L. Kolin, D. Ronis, and P. W. Wiseman (2006)
Biophys. J. 91, 3061-3075
   Abstract »    Full Text »    PDF »
A mechanism to signal receptor-substrate interactions with luminescent quantum dots.
I. Yildiz, M. Tomasulo, and F. M. Raymo (2006)
PNAS 103, 11457-11460
   Abstract »    Full Text »    PDF »
Single plasma membrane K+ channel detection by using dual-color quantum dot labeling.
V. Nechyporuk-Zloy, C. Stock, H. Schillers, H. Oberleithner, and A. Schwab (2006)
Am J Physiol Cell Physiol 291, C266-C269
   Abstract »    Full Text »    PDF »
Membrane Lateral Diffusion and Capture of CFTR within Transient Confinement Zones.
I. R. Bates, B. Hebert, Y. Luo, J. Liao, A. I. Bachir, D. L. Kolin, P. W. Wiseman, and J. W. Hanrahan (2006)
Biophys. J. 91, 1046-1058
   Abstract »    Full Text »    PDF »
Nanodiagnostics: A New Frontier for Clinical Laboratory Medicine.
H. M.E. Azzazy, M. M.H. Mansour, and S. C. Kazmierczak (2006)
Clin. Chem. 52, 1238-1246
   Abstract »    Full Text »    PDF »
Living cells as test tubes..
X. S. Xie, J. Yu, and W. Y. Yang (2006)
Science 312, 228-230
   Abstract »    Full Text »    PDF »
Homogeneous point mutation detection by quantum dot-mediated two-color fluorescence coincidence analysis.
H.-C. Yeh, Y.-P. Ho, I.-M. Shih, and T.-H. Wang (2006)
Nucleic Acids Res. 34, e35
   Abstract »    Full Text »    PDF »
Quantum dots to monitor RNAi delivery and improve gene silencing.
A. A. Chen, A. M. Derfus, S. R. Khetani, and S. N. Bhatia (2005)
Nucleic Acids Res. 33, e190
   Abstract »    Full Text »    PDF »
Research Strategies for Safety Evaluation of Nanomaterials, Part III: Nanoscale Technologies for Assessing Risk and Improving Public Health.
D. M. Balshaw, M. Philbert, and W. A. Suk (2005)
Toxicol. Sci. 88, 298-306
   Abstract »    Full Text »    PDF »
V{alpha}24-Invariant NKT Cells from Patients with Allergic Asthma Express CCR9 at High Frequency and Induce Th2 Bias of CD3+ T Cells upon CD226 Engagement.
Y. Sen, B. Yongyi, H. Yuling, X. Luokun, H. Li, X. Jie, D. Tao, Z. Gang, L. Junyan, H. Chunsong, et al. (2005)
J. Immunol. 175, 4914-4926
   Abstract »    Full Text »    PDF »
Nanotechnology for the biologist.
S. E. McNeil (2005)
J. Leukoc. Biol. 78, 585-594
   Abstract »    Full Text »    PDF »
Real-Time Detection of Virus Particles and Viral Protein Expression with Two-Color Nanoparticle Probes.
A. Agrawal, R. A. Tripp, L. J. Anderson, and S. Nie (2005)
J. Virol. 79, 8625-8628
   Abstract »    Full Text »    PDF »
Targeting quantum dots to surface proteins in living cells with biotin ligase.
M. Howarth, K. Takao, Y. Hayashi, and A. Y. Ting (2005)
PNAS 102, 7583-7588
   Abstract »    Full Text »    PDF »
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics.
X. Michalet, F. F. Pinaud, L. A. Bentolila, J. M. Tsay, S. Doose, J. J. Li, G. Sundaresan, A. M. Wu, S. S. Gambhir, and S. Weiss (2005)
Science 307, 538-544
   Abstract »    Full Text »    PDF »
Simultaneous Determination of {alpha}-Fetoprotein and Free {beta}-Human Chorionic Gonadotropin by Element-Tagged Immunoassay with Detection by Inductively Coupled Plasma Mass Spectrometry.
S. Zhang, C. Zhang, Z. Xing, and X. Zhang (2004)
Clin. Chem. 50, 1214-1221
   Abstract »    Full Text »    PDF »
A fluorescence resonance energy transfer-derived structure of a quantum dot-protein bioconjugate nanoassembly.
I. L. Medintz, J. H. Konnert, A. R. Clapp, I. Stanish, M. E. Twigg, H. Mattoussi, J. M. Mauro, and J. R. Deschamps (2004)
PNAS 101, 9612-9617
   Abstract »    Full Text »    PDF »
Dual FRET molecular beacons for mRNA detection in living cells.
P. J. Santangelo, B. Nix, A. Tsourkas, and G. Bao (2004)
Nucleic Acids Res. 32, e57
   Abstract »    Full Text »    PDF »
Semiconductor nanocrystal probes for human metaphase chromosomes.
Y. Xiao and P. E. Barker (2004)
Nucleic Acids Res. 32, e28
   Abstract »    Full Text »    PDF »
Application of Quantum Dots as Probes for Correlative Fluorescence, Conventional, and Energy-filtered Transmission Electron Microscopy.
R. Nisman, G. Dellaire, Y. Ren, R. Li, and D. P. Bazett-Jones (2004)
J. Histochem. Cytochem. 52, 13-18
   Abstract »    Full Text »    PDF »
Quantum Dots as a Novel Immunofluorescent Detection System for Cryptosporidium parvum and Giardia lamblia.
L. Zhu, S. Ang, and W.-T. Liu (2004)
Appl. Envir. Microbiol. 70, 597-598
   Abstract »    Full Text »    PDF »
Combined Tyramide Signal Amplification and Quantum Dots for Sensitive and Photostable Immunofluorescence Detection.
J. M. Ness, R. S. Akhtar, C. B. Latham, and K. A. Roth (2003)
J. Histochem. Cytochem. 51, 981-987
   Abstract »    Full Text »    PDF »
Quantum Dots as Strain- and Metabolism-Specific Microbiological Labels.
J. A. Kloepfer, R. E. Mielke, M. S. Wong, K. H. Nealson, G. Stucky, and J. L. Nadeau (2003)
Appl. Envir. Microbiol. 69, 4205-4213
   Abstract »    Full Text »    PDF »
Water-Soluble Quantum Dots for Multiphoton Fluorescence Imaging in Vivo.
D. R. Larson, W. R. Zipfel, R. M. Williams, S. W. Clark, M. P. Bruchez, F. W. Wise, and W. W. Webb (2003)
Science 300, 1434-1436
   Abstract »    Full Text »    PDF »
Multiplexed SNP genotyping using the QbeadTM system: a quantum dot-encoded microsphere-based assay.
H. Xu, M. Y. Sha, E. Y. Wong, J. Uphoff, Y. Xu, J. A. Treadway, A. Truong, E. O'Brien, S. Asquith, M. Stubbins, et al. (2003)
Nucleic Acids Res. 31, e43
   Abstract »    Full Text »    PDF »
Rare earth-doped glass microbarcodes.
M. J. Dejneka, A. Streltsov, S. Pal, A. G. Frutos, C. L. Powell, K. Yost, P. K. Yuen, U. Muller, and J. Lahiri (2003)
PNAS 100, 389-393
   Abstract »    Full Text »    PDF »
In Vivo Imaging of Quantum Dots Encapsulated in Phospholipid Micelles.
B. Dubertret, P. Skourides, D. J. Norris, V. Noireaux, A. H. Brivanlou, and A. Libchaber (2002)
Science 298, 1759-1762
   Abstract »    Full Text »    PDF »
Nanocrystal targeting in vivo.
M. E. Akerman, W. C. W. Chan, P. Laakkonen, S. N. Bhatia, and E. Ruoslahti (2002)
PNAS 99, 12617-12621
   Abstract »    Full Text »    PDF »
Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection.
Y. C. Cao, R. Jin, and C. A. Mirkin (2002)
Science 297, 1536-1540
   Abstract »    Full Text »    PDF »
Photothermal Imaging of Nanometer-Sized Metal Particles Among Scatterers.
D. Boyer, P. Tamarat, A. Maali, B. Lounis, and M. Orrit (2002)
Science 297, 1160-1163
   Abstract »    Full Text »    PDF »
Emulating biology: Building nanostructures from the bottom up.
N. C. Seeman and A. M. Belcher (2002)
PNAS 99, 6451-6455
   Abstract »    Full Text »    PDF »
Array-Based Electrical Detection of DNA with Nanoparticle Probes.
S.-J. Park, T. A. Taton, and C. A. Mirkin (2002)
Science 295, 1503-1506
   Abstract »    Full Text »    PDF »
A new class of homogeneous nucleic acid probes based on specific displacement hybridization.
Q. Li, G. Luan, Q. Guo, and J. Liang (2002)
Nucleic Acids Res. 30, e5
   Abstract »    Full Text »    PDF »
Photoinduced Conversion of Silver Nanospheres to Nanoprisms.
R. Jin, Y. Cao, C. A. Mirkin, K. L. Kelly, G. C. Schatz, and J. G. Zheng (2001)
Science 294, 1901-1903
   Abstract »    Full Text »    PDF »
Submicrometer Metallic Barcodes.
S. R. Nicewarner-Pena, R. G. Freeman, B. D. Reiss, L. He, D. J. Pena, I. D. Walton, R. Cromer, C. D. Keating, and M. J. Natan (2001)
Science 294, 137-141
   Abstract »    Full Text »    PDF »
Supersensitive Time-resolved Immunofluorometric Assay of Free Prostate-specific Antigen with Nanoparticle Label Technology.
T. Soukka, J. Paukkunen, H. Harma, S. Lonnberg, H. Lindroos, and T. Lovgren (2001)
Clin. Chem. 47, 1269-1278
   Abstract »    Full Text »    PDF »
Visualization of oligonucleotide probes and point mutations in interphase nuclei and DNA fibers using rolling circle DNA amplification.
X.-b. Zhong, P. M. Lizardi, X.-h. Huang, P. L. Bray-Ward, and D. C. Ward (2001)
PNAS 98, 3940-3945
   Abstract »    Full Text »    PDF »
Europium Nanoparticles and Time-resolved Fluorescence for Ultrasensitive Detection of Prostate-specific Antigen.
H. Harma, T. Soukka, and T. Lovgren (2001)
Clin. Chem. 47, 561-568
   Abstract »    Full Text »    PDF »
Ultrahigh-resolution multicolor colocalization of single fluorescent probes.
T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss (2000)
PNAS
   Abstract »    Full Text »
Single-target molecule detection with nonbleaching multicolor optical immunolabels.
S. Schultz, D. R. Smith, J. J. Mock, and D. A. Schultz (2000)
PNAS 97, 996-1001
   Abstract »    Full Text »    PDF »
Nucleic Acid Detection Technologies — Labels, Strategies, and Formats.
L. J. Kricka (1999)
Clin. Chem. 45, 453-458
   Abstract »    Full Text »    PDF »
Linearly Polarized Emission from Colloidal Semiconductor Quantum Rods.
J. Hu, L.-s. Li, W. Yang, L. Manna, L.-w. Wang, and A. P. Alivisatos (2001)
Science 292, 2060-2063
   Abstract »    Full Text »    PDF »
Ultrahigh-resolution multicolor colocalization of single fluorescent probes.
T. D. Lacoste, X. Michalet, F. Pinaud, D. S. Chemla, A. P. Alivisatos, and S. Weiss (2000)
PNAS 97, 9461-9466
   Abstract »    Full Text »    PDF »
Emulating biology: Building nanostructures from the bottom up.
N. C. Seeman and A. M. Belcher (2002)
PNAS 99, 6451-6455
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)