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Science 18 September 1998:
Vol. 281. no. 5384, pp. 1857 - 1859
DOI: 10.1126/science.281.5384.1857
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Reports

Fertilization Defects in Sperm from Mice Lacking Fertilin beta  

Chunghee Cho, Donna O'Dell Bunch, Jean-Emmanuel Faure, * Eugenia H. Goulding, Edward M. Eddy, Paul Primakoff, Diana G. Myles dagger

Fertilin, a member of the ADAM family, is found on the plasma membrane of mammalian sperm. Sperm from mice lacking fertilin beta  were shown to be deficient in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida. Egg activation was unaffected. The results are consistent with a direct role of fertilin in sperm-egg plasma membrane interaction. Fertilin could also have a direct role in sperm-zona binding or oviduct migration; alternatively, the effects on these functions could result from the absence of fertilin activity during spermatogenesis.

C. Cho, J.-E. Faure, P. Primakoff, D. G. Myles, Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA. D. O. Bunch, E. H. Goulding, E. M. Eddy, Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Science, National Institutes of Health, Research Triangle Park, NC 27709, USA.
*   Present address: Laboratory of Reproduction and Development of Plants, Ecole Normale Superieure de Lyon, 69364 Lyon, France.

dagger    To whom correspondence should be addressed. E-mail: dgmyles{at}ucdavis.edu

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