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Science 18 September 1998:
Vol. 281. no. 5384, pp. 1854 - 1857
DOI: 10.1126/science.281.5384.1854
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Reports

Regulation of Meiotic S Phase by Ime2 and a Clb5,6-Associated Kinase in Saccharomyces cerevisiae

Léon Dirick, Loretta Goetsch, Gustav Ammerer, Breck Byers *

Cyclin-dependent kinase (Cdk) mutations that prevent entry into the mitotic cell cycle of budding yeast fail to block meiotic DNA replication, suggesting there may be fundamental differences between these pathways. However, S phase in meiosis was found to depend on the same B-type cyclins (Clb5 and Clb6) as it does in mitosis. Meiosis differs instead in the mechanism that controls removal of the Cdk inhibitor Sic1. Destruction of Sic1 and activation of a Clb5-dependent kinase in meiotic cells required the action of the meiosis-specific protein kinase Ime2, thereby coupling early meiotic gene expression to control of DNA replication for meiosis.

L. Dirick, L. Goetsch, B. Byers, Department of Genetics, Box 357360, University of Washington, Seattle, WA 98195-7360, USA. G. Ammerer, Department of Biochemistry and Molecular Cell Biology, Ludwig Boltzmann Institute, University of Vienna, Dr. Bohr-Gasse, 9, A-1030 Vienna, Austria.
*   To whom correspondence should be addressed.

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