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Science 7 August 1998:
Vol. 281. no. 5378, pp. 821 - 824
DOI: 10.1126/science.281.5378.821
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Reports

Role of Phosphorylation in Regulation of the Assembly of Endocytic Coat Complexes

Vladimir I. Slepnev, Gian-Carlo Ochoa, Margaret H. Butler, Detlev Grabs, Pietro De Camilli *

Clathrin-mediated endocytosis involves cycles of assembly and disassembly of clathrin coat components and their accessory proteins. Dephosphorylation of rat brain extract was shown to promote the assembly of dynamin 1, synaptojanin 1, and amphiphysin into complexes that also included clathrin and AP-2. Phosphorylation of dynamin 1 and synaptojanin 1 inhibited their binding to amphiphysin, whereas phosphorylation of amphiphysin inhibited its binding to AP-2 and clathrin. Thus, phosphorylation regulates the association and dissociation cycle of the clathrin-based endocytic machinery, and calcium-dependent dephosphorylation of endocytic proteins could prepare nerve terminals for a burst of endocytosis.

Howard Hughes Medical Institute and Department of Cell Biology, Yale University School of Medicine, 295 Congress Avenue, New Haven, CT 06510, USA.
*   To whom correspondence should be addressed. E-mail: pietro.decamilli{at}yale.edu

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Tandem Arrangement of the Clathrin and AP-2 Binding Domains in Amphiphysin 1 and Disruption of Clathrin Coat Function by Amphiphysin Fragments Comprising These Sites.
V. I. Slepnev, G.-C. Ochoa, M. H. Butler, and P. De Camilli (2000)
J. Biol. Chem. 275, 17583-17589
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Sustained stimulation shifts the mechanism of endocytosis from dynamin-1-dependent rapid endocytosis to clathrin- and dynamin-2-mediated slow endocytosis in chromaffin cells.
C. R. Artalejo, A. Elhamdani, and H. C. Palfrey (2002)
PNAS 99, 6358-6363
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Calcineurin controls nerve activity-dependent specification of slow skeletal muscle fibers but not muscle growth.
A. L. Serrano, M. Murgia, G. Pallafacchina, E. Calabria, P. Coniglio, T. Lomo, and S. Schiaffino (2001)
PNAS 98, 13108-13113
   Abstract »    Full Text »    PDF »
The conserved Pkh-Ypk kinase cascade is required for endocytosis in yeast.
A. K.A. deHart, J. D. Schnell, D. A. Allen, and L. Hicke (2002)
J. Cell Biol. 156, 241-248
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Identification of an adaptor-associated kinase, AAK1, as a regulator of clathrin-mediated endocytosis.
S. D. Conner and S. L. Schmid (2002)
J. Cell Biol. 156, 921-929
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G protein-coupled receptor/arrestin3 modulation of the endocytic machinery.
F. Santini, I. Gaidarov, and J. H. Keen (2002)
J. Cell Biol. 156, 665-676
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Phosphorylation of the AP2 {micro} subunit by AAK1 mediates high affinity binding to membrane protein sorting signals.
D. Ricotta, S. D. Conner, S. L. Schmid, K. von Figura, and S. Honing (2002)
J. Cell Biol. 156, 791-795
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