Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 31 July 1998:
Vol. 281. no. 5377, pp. 706 - 709
DOI: 10.1126/science.281.5377.706
Prev | Table of Contents | Next

Reports

Pioneer Axon Guidance by UNC-129, a C. elegans TGF-beta

Antonio Colavita, Srikant Krishna, Hong Zheng, Richard W. Padgett, Joseph G. Culotti *

The unc-129 gene, like the unc-6 netrin gene, is required to guide pioneer motoraxons along the dorsoventral axis of Caenorhabditis elegans. unc-129 encodes a member of the transforming growth factor-beta (TGF-beta ) superfamily of secreted signaling molecules and is expressed in dorsal, but not ventral, rows of body wall muscles. Ectopic expression of UNC-129 from ventral body wall muscle disrupts growth cone and cell migrations that normally occur along the dorsoventral axis. Thus, UNC-129 mediates expression of dorsoventral polarity information required for axon guidance and guided cell migrations in C. elegans.

A. Colavita, H. Zheng, J. G. Culotti, Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada, and Department of Molecular and Medical Genetics, University of Toronto, Ontario M5S 1A8, Canada. S. Krishna and R. W. Padgett, Waksman Institute, Department of Molecular Biology and Biochemistry, and the Cancer Institute of New Jersey, Rutgers University, Piscataway, NJ 08854-8020, USA.
*   To whom correspondence should be addressed. E-mail: culotti{at}mshri.on.ca

Read the Full Text


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
TGF{beta} signals regulate axonal development through distinct Smad-independent mechanisms.
J. Ng (2008)
Development 135, 4025-4035
   Abstract »    Full Text »    PDF »
unc-44 Ankyrin and stn-2 {gamma}-Syntrophin Regulate sax-7 L1CAM Function in Maintaining Neuronal Positioning in Caenorhabditis elegans.
S. Zhou, K. Opperman, X. Wang, and L. Chen (2008)
Genetics 180, 1429-1443
   Abstract »    Full Text »    PDF »
Distinct cellular and molecular mechanisms mediate initial axon development and adult-stage axon regeneration in C. elegans.
C. V. Gabel, F. Antoine, C.-F. Chuang, A. D. T. Samuel, and C. Chang (2008)
Development 135, 1129-1136
   Abstract »    Full Text »    PDF »
Axon guidance genes identified in a large-scale RNAi screen using the RNAi-hypersensitive Caenorhabditis elegans strain nre-1(hd20) lin-15b(hd126).
C. Schmitz, P. Kinge, and H. Hutter (2007)
PNAS 104, 834-839
   Abstract »    Full Text »    PDF »
The metalloprotease Tolloid-related and its TGF-{beta}-like substrate Dawdle regulate Drosophila motoneuron axon guidance..
M. Serpe and M. B. O'Connor (2006)
Development 133, 4969-4979
   Abstract »    Full Text »    PDF »
The divergent TGF-{beta} ligand Dawdle utilizes an activin pathway to influence axon guidance in Drosophila..
L. Parker, J. E. Ellis, M. Q. Nguyen, and K. Arora (2006)
Development 133, 4981-4991
   Abstract »    Full Text »    PDF »
The Caenorhabditis elegans P21-activated kinases are differentially required for UNC-6/netrin-mediated commissural motor axon guidance.
M. Lucanic, M. Kiley, N. Ashcroft, N. L'Etoile, and H.-J. Cheng (2006)
Development 133, 4549-4559
   Abstract »    Full Text »    PDF »
Genes That Control Ray Sensory Neuron Axon Development in the Caenorhabditis elegans Male.
L. Jia and S. W. Emmons (2006)
Genetics 173, 1241-1258
   Abstract »    Full Text »    PDF »
SRC-1, a non-receptor type of protein tyrosine kinase, controls the direction of cell and growth cone migration in C. elegans.
B. Itoh, T. Hirose, N. Takata, K. Nishiwaki, M. Koga, Y. Ohshima, and M. Okada (2005)
Development 132, 5161-5172
   Abstract »    Full Text »    PDF »
Novel brain wiring functions for classical morphogens: a role as graded positional cues in axon guidance.
F. Charron and M. Tessier-Lavigne (2005)
Development 132, 2251-2262
   Abstract »    Full Text »    PDF »
Heparan 2-O-sulfotransferase, hst-2, is essential for normal cell migration in Caenorhabditis elegans.
T. Kinnunen, Z. Huang, J. Townsend, M. M. Gatdula, J. R. Brown, J. D. Esko, and J. E. Turnbull (2005)
PNAS 102, 1507-1512
   Abstract »    Full Text »    PDF »
Mapping Netrin Receptor Binding Reveals Domains of Unc5 Regulating Its Tyrosine Phosphorylation.
R. P. Kruger, J. Lee, W. Li, and K.-L. Guan (2004)
J. Neurosci. 24, 10826-10834
   Abstract »    Full Text »    PDF »
Hedgehog and Decapentaplegic instruct polarized growth of cell extensions in the Drosophila trachea.
K. Kato, T. Chihara, and S. Hayashi (2004)
Development 131, 5253-5261
   Abstract »    Full Text »    PDF »
Caenorhabditis elegans VEM-1, a Novel Membrane Protein, Regulates the Guidance of Ventral Nerve Cord-Associated Axons.
E. Runko and Z. Kaprielian (2004)
J. Neurosci. 24, 9015-9026
   Abstract »    Full Text »    PDF »
Extracellular cues and pioneers act together to guide axons in the ventral cord of C. elegans.
H. Hutter (2003)
Development 130, 5307-5318
   Abstract »    Full Text »    PDF »
Myostatin Signals through a Transforming Growth Factor {beta}-Like Signaling Pathway To Block Adipogenesis.
A. Rebbapragada, H. Benchabane, J. L. Wrana, A. J. Celeste, and L. Attisano (2003)
Mol. Cell. Biol. 23, 7230-7242
   Abstract »    Full Text »    PDF »
Functional Analysis of the Caenorhabditis elegans UNC-73B PH Domain Demonstrates a Role in Activation of the Rac GTPase In Vitro and Axon Guidance In Vivo.
T. J. Kubiseski, J. Culotti, and T. Pawson (2003)
Mol. Cell. Biol. 23, 6823-6835
   Abstract »    Full Text »    PDF »
Talin loss-of-function uncovers roles in cell contractility and migration in C. elegans.
E. J. Cram, S. G. Clark, and J. E. Schwarzbauer (2003)
J. Cell Sci. 116, 3871-3878
   Abstract »    Full Text »    PDF »
C. elegans ZAG-1, a Zn-finger-homeodomain protein, regulates axonal development and neuronal differentiation.
S. G. Clark and C. Chiu (2003)
Development 130, 3781-3794
   Abstract »    Full Text »    PDF »
Modeling human peroxisome biogenesis disorders in the nematode Caenorhabditis elegans.
H. Thieringer, B. Moellers, G. Dodt, W.-H. Kunau, and M. Driscoll (2003)
J. Cell Sci. 116, 1797-1804
   Abstract »    Full Text »    PDF »
The C. elegans even-skipped homologue, vab-7, specifies DB motoneurone identity and axon trajectory.
B. Esmaeili, J. M. Ross, C. Neades, D. M. Miller III, and J. Ahringer (2003)
Development 129, 853-862
   Abstract »    Full Text »    PDF »
The DAF-7 TGF-beta signaling pathway regulates chemosensory receptor gene expression in C. elegans.
K. M. Nolan, T. R. Sarafi-Reinach, J. G. Horne, A. M. Saffer, and P. Sengupta (2002)
Genes & Dev. 16, 3061-3073
   Abstract »    Full Text »    PDF »
Identification of Domains of Netrin UNC-6 that Mediate Attractive and Repulsive Guidance and Responses from Cells and Growth Cones.
Y.-s. Lim and W. G. Wadsworth (2002)
J. Neurosci. 22, 7080-7087
   Abstract »    Full Text »    PDF »
The C Domain of Netrin UNC-6 Silences Calcium/Calmodulin-Dependent Protein Kinase- and Diacylglycerol-Dependent Axon Branching in Caenorhabditis elegans.
Q. Wang and W. G. Wadsworth (2002)
J. Neurosci. 22, 2274-2282
   Abstract »    Full Text »    PDF »
Semaphorin 1a and semaphorin 1b are required for correct epidermal cell positioning and adhesion during morphogenesis in C. elegans.
V. E. Ginzburg, P. J. Roy, and J. G. Culotti (2002)
Development 129, 2065-2078
   Abstract »    Full Text »    PDF »
The Nc1/Endostatin Domain of Caenorhabditis elegans Type Xviii Collagen Affects Cell Migration and Axon Guidance.
B. D. Ackley, J. R. Crew, H. Elamaa, T. Pihlajaniemi, C. J. Kuo, and J. M. Kramer (2001)
J. Cell Biol. 152, 1219-1232
   Abstract »    Full Text »    PDF »
Mutation and Analysis of Dan, the Founding Member of the Dan Family of Transforming Growth Factor {beta} Antagonists.
M. S. Dionne, W. C. Skarnes, and R. M. Harland (2001)
Mol. Cell. Biol. 21, 636-643
   Abstract »    Full Text »
Identification of tgh-2, a Filarial Nematode Homolog of Caenorhabditis elegans daf-7 and Human Transforming Growth Factor beta , Expressed in Microfilarial and Adult Stages of Brugia malayi.
N. Gomez-Escobar, W. F. Gregory, and R. M. Maizels (2000)
Infect. Immun. 68, 6402-6410
   Abstract »    Full Text »    PDF »
The Forkhead transcription factor UNC-130 is required for the graded spatial expression of the UNC-129 TGF-beta guidance factor in C. elegans.
B. Nash, A. Colavita, H. Zheng, P. J. Roy, and J. G. Culotti (2000)
Genes & Dev. 14, 2486-2500
   Abstract »    Full Text »
Positioning of Longitudinal Nerves in C. elegans by Nidogen.
S. Kim and W. G. Wadsworth (2000)
Science 288, 150-154
   Abstract »    Full Text »
Netrin 1 acts as an attractive or as a repulsive cue for distinct migrating neurons during the development of the cerebellar system.
S Alcantara, M Ruiz, F De Castro, E Soriano, and C Sotelo (2000)
Development 127, 1359-1372
   Abstract »    PDF »
Regulation of the UNC-5 netrin receptor initiates the first reorientation of migrating distal tip cells in Caenorhabditis elegans.
M Su, D. Merz, M. Killeen, Y Zhou, H Zheng, J. Kramer, E. Hedgecock, and J. Culotti (2000)
Development 127, 585-594
   Abstract »    PDF »
mab-20 encodes Semaphorin-2a and is required to prevent ectopic cell contacts during epidermal morphogenesis in Caenorhabditis elegans.
P. Roy, H Zheng, C. Warren, and J. Culotti (2000)
Development 127, 755-767
   Abstract »    PDF »
Complex Patterns of Alternative Splicing Mediate the Spatial and Temporal Distribution of Perlecan/UNC-52 in Caenorhabditis elegans.
G. P. Mullen, T. M. Rogalski, J. A. Bush, P. R. Gorji, and D. G. Moerman (1999)
Mol. Biol. Cell 10, 3205-3221
   Abstract »    Full Text »
Netrin UNC-6 and the Regulation of Branching and Extension of Motoneuron Axons from the Ventral Nerve Cord of Caenorhabditis elegans.
Y.-S. Lim, S. Mallapur, G. Kao, X.-C. Ren, and W. G. Wadsworth (1999)
J. Neurosci. 19, 7048-7056
   Abstract »    Full Text »    PDF »
A Single Growth Cone is Capable of Integrating Simultaneously Presented and Functionally Distinct Molecular Cues during Target Recognition.
D. Rose and A. Chiba (1999)
J. Neurosci. 19, 4899-4906
   Abstract »    Full Text »    PDF »
Mammalian Homologue of the Caenorhabditis elegans UNC-76 Protein Involved in Axonal Outgrowth Is a Protein Kinase C {zeta}-interacting Protein.
S. Kuroda, N. Nakagawa, C. Tokunaga, K. Tatematsu, and K. Tanizawa (1999)
J. Cell Biol. 144, 403-411
   Abstract »    Full Text »    PDF »
The zebrafish diwanka gene controls an early step of motor growth cone migration.
J Zeller and M Granato (1999)
Development 126, 3461-3472
   Abstract »    PDF »
Regulation of body length and male tail ray pattern formation of Caenorhabditis elegans by a member of TGF-beta family.
K Morita, K. Chow, and N Ueno (1999)
Development 126, 1337-1347
   Abstract »    PDF »
Commissure formation in the embryonic CNS of Drosophila.
T Hummel, K Schimmelpfeng, and C Klambt (1999)
Development 126, 771-779
   Abstract »    PDF »
A BMP homolog acts as a dose-dependent regulator of body size and male tail patterning in Caenorhabditis elegans.
Y Suzuki, M. Yandell, P. Roy, S Krishna, C Savage-Dunn, R. Ross, R. Padgett, and W. Wood (1999)
Development 126, 241-250
   Abstract »    PDF »
Specificity of TGFbeta signaling is conferred by distinct type I receptors and their associated SMAD proteins in Caenorhabditis elegans.
S Krishna, L. Maduzia, and R. Padgett (1999)
Development 126, 251-260
   Abstract »    PDF »
Netrin Stimulates Tyrosine Phosphorylation of the UNC-5 Family of Netrin Receptors and Induces Shp2 Binding to the RCM Cytodomain.
J. Tong, M. Killeen, R. Steven, K. L. Binns, J. Culotti, and T. Pawson (2001)
J. Biol. Chem. 276, 40917-40925
   Abstract »    Full Text »    PDF »
Roles for beta pat-3 Integrins in Development and Function of Caenorhabditis elegans Muscles and Gonads.
M. Lee, E. J. Cram, B. Shen, and J. E. Schwarzbauer (2001)
J. Biol. Chem. 276, 36404-36410
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)