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Science 24 July 1998:
Vol. 281. no. 5376, pp. 572 - 575
DOI: 10.1126/science.281.5376.572
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Reports

Fidelity of T Cell Activation Through Multistep T Cell Receptor zeta  Phosphorylation

Ellen Neumeister Kersh, Andrey S. Shaw, Paul M. Allen *

The T cell receptor (TCR) alpha beta heterodimer interacts with its ligands with high specificity, but surprisingly low affinity. The role of the zeta  component of the murine TCR in contributing to the fidelity of antigen recognition was examined. With sequence-specific phosphotyrosine antibodies, it was found that zeta  undergoes a series of ordered phosphorylation events upon TCR engagement. Completion of phosphorylation steps is dependent on the nature of the TCR ligand. Thus, the phosphorylation steps establish thresholds for T cell activation. This study documents the sophisticated molecular events that follow the engagement of a low-affinity receptor.

Center for Immunology and Department of Pathology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, MO 63110, USA.
*   To whom correspondence should be addressed. E-mail: allen{at}immunology.wustl.edu

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