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Science 24 July 1998:
Vol. 281. no. 5376, pp. 565 - 568
DOI: 10.1126/science.281.5376.565
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Reports

Delivery of Epitopes by the Salmonella Type III Secretion System for Vaccine Development

Holger Rüssmann, * Homayoun Shams, Fernando Poblete, Yixin Fu, Jorge E. Galán, dagger Ruben O. Donis

Avirulent strains of Salmonella typhimurium are being considered as antigen delivery vectors. During its intracellular stage in the host, S. typhimurium resides within a membrane-bound compartment and is not an efficient inducer of class I-restricted immune responses. Viral epitopes were successfully delivered to the host-cell cytosol by using the type III protein secretion system of S. typhimurium. This resulted in class I-restricted immune responses that protected vaccinated animals against lethal infection. This approach may allow the efficient use of S. typhimurium as an antigen delivery system to control infections by pathogens that require this type of immune response for protection.

H. Rüssmann, Y. Fu, J. E. Galán, Department of Molecular Genetics and Microbiology, School of Medicine, State University of New York at Stony Brook, Stony Brook, NY 11794-5222, USA. H. Shams, F. Poblete, R. O. Donis, Department of Veterinary and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68583-0905, USA.
*   Present address: Max von Pettenkofer-Institut, Pettenkofer Strasse 9a, 80336 Munich, Germany.

dagger    To whom correspondence should be addressed. E-mail: galan{at}asterix.bio.sunysb.edu

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